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iFluor® 430 succinimidyl ester
AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, and 633 nm). iFluor® 430 dyes are designed to be a superior replacement for Alexa Fluor® 430 labeling dye (Alexa Fluor® is the trademark of Invitrogen). iFluor® 430 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups. Under the same conditions, iFluor® 430 dye conjugates are significantly brighter than the corresponding bioconjugates of Alexa Fluor 430 with much stronger absorption, making the iFluor 430 conjugates much more sensitive.
<p>HeLa cells were incubated with mouse anti-tubulin followed by AAT&rsquo;s iFluor<sup>TM</sup>&nbsp;430 goat anti-mouse IgG conjugate (Left) or goat anti-mouse IgG conjugated with Alexa Fluor<sup>&reg;</sup>&nbsp;430&nbsp; (Right), respectively.</p>
<p>HeLa cells were incubated with mouse anti-tubulin followed by AAT&rsquo;s iFluor<sup>TM</sup>&nbsp;430 goat anti-mouse IgG conjugate (Left) or goat anti-mouse IgG conjugated with Alexa Fluor<sup>&reg;</sup>&nbsp;430&nbsp; (Right), respectively.</p>
CatalogSize
Price
Quantity
10521 mg
Price
71052100 ug
Price
715235 mg
Price
7157310 mg
Price
 
Physical properties

Molecular weight688.79
SolventDMSO
Spectral properties

Correction factor (260 nm)0.68
Correction factor (280 nm)0.3
Extinction coefficient (cm -1 M -1)
40000
1
Excitation (nm)433
Emission (nm)498
Quantum yield
0.78
1
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Page updated on October 8, 2025