iFluor® 430 succinimidyl ester

iFluor® 430 Succinimidyl Ester is an amine-reactive fluorescent dye for covalent labeling of proteins, particularly antibodies, in fluorescence-based research applications.

Amine-Reactive Functionality: NHS ester selectively reacts with primary amines, including lysine residues.
Spectral Properties: Excitation at 433 nm and emission at 497 nm.
Fluorescence Performance: Bright, photostable fluorescence with minimal quenching.
Enhanced Brightness: iFluor® 430 conjugates exhibit stronger fluorescence than comparable Alexa Fluor® 430 conjugates under manufacturer-tested conditions.
Applications: Suitable for fluorescent conjugation of antibodies and other amine-containing proteins.

<p>HeLa cells were incubated with mouse anti-tubulin followed by AAT’s iFluor<sup>TM</sup> 430 goat anti-mouse IgG conjugate (Left) or goat anti-mouse IgG conjugated with Alexa Fluor<sup>®</sup> 430  (Right), respectively.</p>

Protocol

Catalog	Size	Price
1052	1 mg	Price
71052	100 ug	Price
71523	5 mg	Price
71573	10 mg	Price

Overview

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, and 633 nm). iFluor® 430 dyes are designed to be a superior replacement for Alexa Fluor® 430 labeling dye (Alexa Fluor® is the trademark of Invitrogen). iFluor® 430 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups. Under the same conditions, iFluor® 430 dye conjugates are significantly brighter than the corresponding bioconjugates of Alexa Fluor 430 with much stronger absorption, making the iFluor® 430 conjugates much more sensitive.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 430 succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	145.182 µL	725.911 µL	1.452 mL	7.259 mL	14.518 mL
5 mM	29.036 µL	145.182 µL	290.364 µL	1.452 mL	2.904 mL
10 mM	14.518 µL	72.591 µL	145.182 µL	725.911 µL	1.452 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	688.79 g/mol	=	mL	x	mM	=	mmol

Citations

View all 3 citations: Citation Explorer

Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification

Authors:

Gao, D and Liu, N and Li, Y and Zhang, Y and Liu, G and others, undefined

Journal:

Metabolomics (Los Angel) (2017): 2153--0769

Suramin inhibits cullin-RING E3 ubiquitin ligases

Authors:

Wu, Kenneth and Chong, Robert A and Yu, Qing and Bai, Jin and Spratt, Donald E and Ching, Kevin and Lee, Chan and Miao, Haibin and Tappin, Inger and Hurwitz, Jerard and others, undefined

Journal:

Proceedings of the National Academy of Sciences (2016): E2011--E2018

Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function

Authors:

Piccard, Helene and Berghmans, Nele and Korpos, Eva and Dillen, Chris and Aelst, Ilse Van and Li, S and ra , undefined and Martens, Erik and Liekens, S and ra , undefined and Noppen, Sam and Damme, Jo Van and others, undefined

Journal:

International Journal of Cancer (2012): E425--E436

References

View all 49 references: Citation Explorer

Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes

Authors:

Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.

Journal:

Mol Biosyst (2011): 1766

Visualizing dengue virus through Alexa Fluor labeling

Authors:

Zhang S, Tan HC, Ooi EE.

Journal:

J Vis Exp. (2011)

Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells

Authors:

Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.

Journal:

Biochem Biophys Res Commun (2011): 211

Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594

Authors:

Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.

Journal:

Zhen Ci Yan Jiu (2011): 262

Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers

Authors:

Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.

Journal:

J Microbiol Methods (2011): 119

Physical properties

Molecular weight	688.79
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.68
Correction factor (280 nm)	0.3
Extinction coefficient (cm ^-1 M ^-1)	20000 ¹
Excitation (nm)	433
Emission (nm)	497
Quantum yield	0.78 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on October 24, 2026