Name: iFluor® 670 succinimidyl ester
Brand: AAT Bioquest
SKU: 1033
Availability: InStock

iFluor® 670 succinimidyl ester

iFluor® 670 succinimidyl ester is an amine-reactive, far-red fluorescent dye designed for covalent labeling of proteins, particularly antibodies, for fluorescence detection applications.

Amine-Reactive Labeling: Exhibits good reactivity and selectivity toward protein amino groups.
Spectral Properties: Excitation/emission maxima of 671/682 nm.
pH Stability: Exhibits pH-independent fluorescence from pH 3 to 11.
Dye Compatibility: Spectrally similar to Cy5B, with higher quantum yield and enhanced photostability.

HeLa cells were incubated with (+ Tubulin) or without (-Tubulin) mouse anti-tubulin followed by iFluor® 670 goat anti-mouse IgG conjugate stain and visualized with Cy5 Filter.

Protocol

Catalog	Size	Price
1033	1 mg	Price
71033	100 ug	Price
71512	5 mg	Price
71562	10 mg	Price

Overview

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, and 633 nm). iFluor® 670 family has spectral properties nearly identical to those of Cy5B. Compared to Cy5B, iFluor® 670 has a much higher fluorescence quantum yield with greatly enhanced photostability. In addition, the fluorescence of iFluor® 670 is pH-insensitive over a broad range, pH 3-11. These spectral characteristics make this new dye family a superior alternative to Cy5B. iFluor® 670 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 670 succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	108.325 µL	541.624 µL	1.083 mL	5.416 mL	10.832 mL
5 mM	21.665 µL	108.325 µL	216.65 µL	1.083 mL	2.166 mL
10 mM	10.832 µL	54.162 µL	108.325 µL	541.624 µL	1.083 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	923.15 g/mol	=	mL	x	mM	=	mmol

Citations

View all 6 citations: Citation Explorer

Photobleaching Comparison of R-Phycoerythrin-Streptavidin and Streptavidin-Alexa Fluor 568 in a Breast Cancer Cell Line

Authors:

Ostad, S. N., Babaei, S., Bayat, A. A., Mahmoudian, J.

Journal:

Monoclon Antib Immunodiagn Immunother (2019): 25-29

A combined solvatochromic shift and TDDFT study probing solute-solvent interactions of blue fluorescent Alexa Fluor 350 dye: Evaluation of ground and excited state dipole moments

Authors:

Patil, M. K., Kotresh, M. G., Inamdar, S. R.

Journal:

Spectrochim Acta A Mol Biomol Spectrosc (2019): 142-152

Comparison between photostability of Alexa Fluor 448 and Alexa Fluor 647 with conventional dyes FITC and APC by flow cytometry

Authors:

Rai, S., Bhardwaj, U., Misra, A., Singh, S., Gupta, R.

Journal:

Int J Lab Hematol (2018): e52-e54

Development of new hCaM-Alexa Fluor((R)) biosensors for a wide range of ligands

Authors:

Velazquez-Lopez, I., Leon-Cruz, E., Pardo, J. P., Sosa-Peinado, A., Gonzalez-Andrade, M.

Journal:

Anal Biochem (2017): 13-22

Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594

Authors:

Cui, J. J., Zhu, X. L., Ji, C. F., Jing, X. H., Bai, W. Z.

Journal:

Zhen Ci Yan Jiu (2011): 262-7

Physical properties

Molecular weight	923.15
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.03
Correction factor (280 nm)	0.033
Extinction coefficient (cm ^-1 M ^-1)	200000 ¹
Excitation (nm)	671
Emission (nm)	682
Quantum yield	0.55 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on November 6, 2026