Name: iFluor® 594 succinimidyl ester
Brand: AAT Bioquest
SKU: 1029
Availability: InStock

iFluor® 594 succinimidyl ester

iFluor® 594 succinimidyl ester is an amine-reactive fluorescent dye designed for covalent labeling of proteins, particularly antibodies, through primary amino groups.

Amine-Reactive Labeling: Exhibits good reactivity and selectivity toward protein amino groups.
Spectral Properties: Excitation/emission maxima of 587/603 nm.
pH Stability: Exhibits pH-independent fluorescence from pH 3 to 11.
Dye Compatibility: Spectrally similar to Texas Red® and Alexa Fluor® 594.

HeLa cells were stained with (Tubulin+) or without (Tubulin-) mouse anti-tubulin and then visualized with iFluor® 594 goat anti-mouse IgG (Left) or with Alexa Fluor® 594 goat anti-mouse IgG (Right).

Protocol

Catalog	Size	Price
1029	1 mg	Price
71029	100 ug	Price
71508	5 mg	Price
71558	10 mg	Price

Overview

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. iFluor® 594 dyes have fluorescence excitation and emission maxima of ~588 nm and ~604 nm respectively. iFluor® 594 family has spectral properties similar to those of Texas Red® and Alexa Fluor® 594 (Texas Red® and Alexa Fluor® 594 are the trademarks of Invitrogen). iFluor® 594 family is pH-independent from pH 3 to 11. These spectral characteristics make this new dye family an excellent alternative to Texas Red® and Alexa Fluor® 594. Compared to Texas Red®, iFluor® 594 is much easier to conjugate with RPE with a much higher conjugation yield, and the resulting RPE-iFluor® 594 tandem has better FRET efficiency. iFluor® 594 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 594 succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	86.176 µL	430.878 µL	861.757 µL	4.309 mL	8.618 mL
5 mM	17.235 µL	86.176 µL	172.351 µL	861.757 µL	1.724 mL
10 mM	8.618 µL	43.088 µL	86.176 µL	430.878 µL	861.757 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1160.42 g/mol	=	mL	x	mM	=	mmol

Citations

View all 14 citations: Citation Explorer

Streamlined stable CHO cell line development using droplet microfluidics with image-based monoclonality assessment

Authors:

Naveen, Kumar Vishven and Tyagi, Akanksha and Ibrahium, Omnia Mohammed Hamid and Fischer, Rainer EAW and Ostafe, Raluca

Journal:

Bioprocess and Biosystems Engineering (2026): 1--17

CLEC3B (tetranectin) expression is associated with vascular localization and tumor aggressiveness in breast cancer

Authors:

Wei, Ching-Ting and Yu, Teng-Hung and Hsu, Chia-Chang and Hsuan, Chin-Feng and Hung, Wei-Chin and Wang, Chao-Ping and Tang, Wei-Hua and Chung, Fu-Mei and Lee, Yau-Jiunn and Chang, Chi-Chang and others

Journal:

International Journal of Medical Sciences (2026): 2715

Streamlined stable CHO cell line development using droplet microfluidics with monoclonality assurance

Authors:

Naveen, Kumar Vishven and Tyagi, Akanksha and Ibrahium, Omnia Mohammed Hamid and Fischer, Rainer EAW and Ostafe, Raluca

Recombinant type I \& III chimeric collagen significantly enhances the vitality of fibroblasts and decelerates the skin aging process

Authors:

Chen, Wei and Wang, Ning and Xie, Xinshan and Liu, Han and Wang, Yiting and Nie, Yanfeng and Chen, Chun and Sun, Huaiqing and Hong, An and Guo, Chaowan and others

Journal:

BMC biotechnology (2025): 1--14

Analyzing Photoreceptor Degeneration and Preservation in a Retinitis Pigmentosa Murine Model

Authors:

de Jesus, Maria Clara da Silva and Cruz, Douglas Penaforte and Le{\'o}n, Greysa Sara{\'\i} Barrios and Cris{\'o}stomo, Millena Costa and Magalh{\~a}es, Camila Feitosa and de Mattos, Aline Estacio Ribeiro and Fragel-Madeira, Lucianne

Journal:

Journal of Visualized Experiments (JoVE) (2025): e68324

References

View all 49 references: Citation Explorer

Visualizing dengue virus through Alexa Fluor labeling

Authors:

Zhang S, Tan HC, Ooi EE.

Journal:

J Vis Exp. (2011)

Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers

Authors:

Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.

Journal:

J Microbiol Methods (2011): 119

Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594

Authors:

Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.

Journal:

Zhen Ci Yan Jiu (2011): 262

Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells

Authors:

Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.

Journal:

Biochem Biophys Res Commun (2011): 211

Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes

Authors:

Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.

Journal:

Mol Biosyst (2011): 1766

Physical properties

Molecular weight	1160.42
Solvent	DMSO

Spectral properties

Absorbance (nm)	587
Correction factor (260 nm)	0.05
Correction factor (280 nm)	0.04
Extinction coefficient (cm ^-1 M ^-1)	200000 ¹
Excitation (nm)	587
Emission (nm)	603
Quantum yield	0.53 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on November 8, 2026