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iFluor® 597 succinimidyl ester
AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, 633, 638, 647, 660, and 802 nm). iFluor® 597 is a unique color for fluorescence imaging and flow cytometry applications. iFluor® 597 is an excellent acceptor dye for preparing PE-tandem dyes. These iFluor® 597 tandem colors offer a set of unique color profiles for spectral flow cytometry. Compared to Alexa Fluor® 594 tandems, iFluor® 597 tandems have improved FRET efficiency and photostability.
Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of PBMC stained with PE/iFlour® 597 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE/iFluor® 597 specific B6-A channel.
Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of PBMC stained with PE/iFlour® 597 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE/iFluor® 597 specific B6-A channel.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
10501 mg
Price
 
Physical properties
Molecular weight1058.29
SolventDMSO
Spectral properties
Absorbance (nm)597
Correction factor (260 nm)0.335
Correction factor (280 nm)0.514
Extinction coefficient (cm -1 M -1)
100000
1
Excitation (nm)598
Emission (nm)618
Quantum yield
0.7
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Page updated on October 8, 2025