Name: iFluor® 546 succinimidyl ester
Brand: AAT Bioquest
SKU: 1048
Availability: InStock

iFluor® 546 succinimidyl ester

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, and 633 nm). iFluor® 546 dyes have fluorescence excitation and emission maxima essentially identical to those of Alexa Fluor® 546 dyes. These spectral characteristics make them excellent alternatives to Alexa Fluor® 546 dyes (Alexa Fluor® is the trademark of Invitrogen). Under the same conditions we tested, iFluor® 546-labeled anti-IgG and streptavidin conjugates are brighter and more photostable than the corresponding Alexa Fluo® 546 conjugates. iFluor® 546 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups.

HeLa cells were stained with (Tubulin+) or without (Tubulin-) mouse anti-tubulin and then visualized with iFluor® 546 goat anti-mouse IgG (Left) or with Alexa Fluor® 546 goat anti-mouse IgG (Right).

Protocol

COA

Catalog	Size	Price
1048	1 mg	Price
71048	100 ug	Price
71521	5 mg	Price
71571	10 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 546 succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	87.305 µL	436.525 µL	873.05 µL	4.365 mL	8.73 mL
5 mM	17.461 µL	87.305 µL	174.61 µL	873.05 µL	1.746 mL
10 mM	8.73 µL	43.652 µL	87.305 µL	436.525 µL	873.05 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1145.41 g/mol	=	mL	x	mM	=	mmol

Citations

View all 8 citations: Citation Explorer

Reconstitution of synaptic junctions orchestrated by teneurin-latrophilin complexes

Authors:

Zhang, Xuchen and Chen, Xudong and Mat{\'u}{\v{s}}, Daniel and S{\"u}dhof, Thomas C

Journal:

Science (2025): 322--329

B-067 Human IgE Monoclonal Antibodies as Unique Tools for Allergy Diagnostics

Authors:

Filep, S and Reid Black, K and Smith, B and Bermingham, M and Pomes, A and Chapman, M

Journal:

Clinical Chemistry (2024): hvae106--429

B-065 Comparative Analysis of 14-3-3$\eta$ Autoantibody Assays on Luminex Platform for Diagnosing Axial Spondyloarthritis

Authors:

Sidhu, N and Maksymowych, WP and Wichuk, S and Marotta, A

Journal:

Clinical Chemistry (2024): hvae106--427

B-066 VeraBIND™ Borrelia Antibody Test: Multiplex Capture, Enrichment, and Detection of Borrelia Burgdorferi Antigens-Specific Immunoglobulins From Saline Oral Rinse Samples

Authors:

Shamsundar, K and Ansari, KI and Bergmann, S and Kleven, K and Soldo, J

Journal:

Clinical Chemistry (2024): hvae106--428

Alternative splicing of latrophilin-3 controls synapse formation

Authors:

Wang, Shuai and DeLeon, Chelsea and Sun, Wenfei and Quake, Stephen R and Roth, Bryan L and S{\"u}dhof, Thomas C

Journal:

Nature (2024): 1--8

References

View all 49 references: Citation Explorer

Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes

Authors:

Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.

Journal:

Mol Biosyst (2011): 1766

Visualizing dengue virus through Alexa Fluor labeling

Authors:

Zhang S, Tan HC, Ooi EE.

Journal:

J Vis Exp. (2011)

Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells

Authors:

Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.

Journal:

Biochem Biophys Res Commun (2011): 211

Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594

Authors:

Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.

Journal:

Zhen Ci Yan Jiu (2011): 262

Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers

Authors:

Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.

Journal:

J Microbiol Methods (2011): 119

Physical properties

Molecular weight	1145.41
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.25
Correction factor (280 nm)	0.15
Extinction coefficient (cm ^-1 M ^-1)	100000 ¹
Excitation (nm)	541
Emission (nm)	557
Quantum yield	0.67 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on October 8, 2025