iFluor® 540 succinimidyl ester
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 853.96 |
Solvent | DMSO |
Spectral properties
Correction Factor (280 nm) | 0.105 |
Excitation (nm) | 540 |
Emission (nm) | 557 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
iFluor® 540 maleimide |
Overview | ![]() ![]() |
See also: iFluor® Dyes and Kits
Molecular weight 853.96 | Correction Factor (280 nm) 0.105 | Excitation (nm) 540 | Emission (nm) 557 |
iFluor® 540 succinimidyl ester is the most convenient reactive form of iFluor® 540 dyes that can be readily used for labeling proteins such as antibodies. iFluor® 540 is a fluorophore from the iFluor® family, which is known for its bright red fluorescence and compatibility with various fluorescence techniques and instruments. When excited with light in the green to yellow range (around 530 to 545 nm), iFluor® 540 emits red fluorescence with an emission peak typically around 560 to 570 nanometers. iFluor® 540 can be conjugated to a variety of biomolecules, including antibodies, proteins, nucleic acids, and small molecules, enabling their visualization and tracking in cells and tissues. It is commonly used in fluorescence microscopy, immunohistochemistry, flow cytometry, and other fluorescence-based assays. The iFluor® dyes, including iFluor® 540, are designed to exhibit high brightness, photostability, and minimal background noise, making them ideal for demanding imaging applications. They are also compatible with multiplexing experiments, allowing for simultaneous visualization of multiple targets using different iFluor® dyes with distinct emission wavelengths.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 540 succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 117.102 µL | 585.508 µL | 1.171 mL | 5.855 mL | 11.71 mL |
5 mM | 23.42 µL | 117.102 µL | 234.203 µL | 1.171 mL | 2.342 mL |
10 mM | 11.71 µL | 58.551 µL | 117.102 µL | 585.508 µL | 1.171 mL |
Molarity calculator
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Spectrum
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Spectral properties
Correction Factor (280 nm) | 0.105 |
Excitation (nm) | 540 |
Emission (nm) | 557 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
iFluor® 350 succinimidyl ester | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
iFluor® 405 succinimidyl ester | 403 | 427 | 370001 | 0.911 | 0.48 | 0.77 |
iFluor® 488 succinimidyl ester | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 514 succinimidyl ester | 511 | 527 | 750001 | 0.831 | 0.265 | 0.116 |
iFluor® 532 succinimidyl ester | 537 | 560 | 900001 | 0.681 | 0.26 | 0.16 |
iFluor® 555 succinimidyl ester | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
iFluor® 594 succinimidyl ester | 588 | 604 | 1800001 | 0.531 | 0.05 | 0.04 |
iFluor® 633 succinimidyl ester | 640 | 654 | 2500001 | 0.291 | 0.062 | 0.044 |
iFluor® 647 succinimidyl ester | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 |
Show More (35) |
References
View all 39 references: Citation Explorer
Gradient-dependent release of the model drug TRITC-dextran from FITC-labeled BSA hydrogel nanocarriers in the hair follicles of porcine ear skin.
Authors: Tran, Ngo Bich Nga Nathalie and Knorr, Fanny and Mak, Wing Cheung and Cheung, Kwan Yee and Richter, Heike and Meinke, Martina and Lademann, Jürgen and Patzelt, Alexa
Journal: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenst (2017): 12-16
Authors: Tran, Ngo Bich Nga Nathalie and Knorr, Fanny and Mak, Wing Cheung and Cheung, Kwan Yee and Richter, Heike and Meinke, Martina and Lademann, Jürgen and Patzelt, Alexa
Journal: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenst (2017): 12-16
Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells.
Authors: Wunsch, Christopher M and Lewis, Janina P
Journal: Journal of visualized experiments : JoVE (2015): e53408
Authors: Wunsch, Christopher M and Lewis, Janina P
Journal: Journal of visualized experiments : JoVE (2015): e53408
Cell-matrix and cell-cell interactions of human gingival fibroblasts on three-dimensional nanofibrous gelatin scaffolds.
Authors: Sachar, Ashneet and Strom, T Amanda and San Miguel, Symone and Serrano, Maria J and Svoboda, Kathy K H and Liu, Xiaohua
Journal: Journal of tissue engineering and regenerative medicine (2014): 862-73
Authors: Sachar, Ashneet and Strom, T Amanda and San Miguel, Symone and Serrano, Maria J and Svoboda, Kathy K H and Liu, Xiaohua
Journal: Journal of tissue engineering and regenerative medicine (2014): 862-73
Sexually dimorphic and developmentally regulated expression of tubulin-specific chaperone protein A in the LMAN of zebra finches.
Authors: Qi, L M and Wade, J
Journal: Neuroscience (2013): 182-90
Authors: Qi, L M and Wade, J
Journal: Neuroscience (2013): 182-90
Mefloquine damage vestibular hair cells in organotypic cultures.
Authors: Yu, Dongzhen and Ding, Dalian and Jiang, Haiyan and Stolzberg, Daniel and Salvi, Richard
Journal: Neurotoxicity research (2011): 51-8
Authors: Yu, Dongzhen and Ding, Dalian and Jiang, Haiyan and Stolzberg, Daniel and Salvi, Richard
Journal: Neurotoxicity research (2011): 51-8
Participation of actin on Giardia lamblia growth and encystation.
Authors: Castillo-Romero, Araceli and Leon-Avila, Gloria and Perez Rangel, Armando and Cortes Zarate, Rafael and Garcia Tovar, Carlos and Hernandez, Jose Manuel
Journal: PloS one (2009): e7156
Authors: Castillo-Romero, Araceli and Leon-Avila, Gloria and Perez Rangel, Armando and Cortes Zarate, Rafael and Garcia Tovar, Carlos and Hernandez, Jose Manuel
Journal: PloS one (2009): e7156
An improved method for the selective detection of fungi in hospital waters by solid phase cytometry.
Authors: De Vos, Muriel M and Nelis, Hans J
Journal: Journal of microbiological methods (2006): 557-65
Authors: De Vos, Muriel M and Nelis, Hans J
Journal: Journal of microbiological methods (2006): 557-65
Clonal domains in postlarval Platynereis dumerilii (Annelida: Polychaeta).
Authors: Ackermann, Christian and Dorresteijn, Adriaan and Fischer, Albrecht
Journal: Journal of morphology (2005): 258-80
Authors: Ackermann, Christian and Dorresteijn, Adriaan and Fischer, Albrecht
Journal: Journal of morphology (2005): 258-80
The influence of etoposide on the distribution of tubulin in human leukemia cell line HL-60.
Authors: Grzanka, Alina and Grzanka, Dariusz
Journal: Medical science monitor : international medical journal of experimental and clinical research (2003): BR66-9
Authors: Grzanka, Alina and Grzanka, Dariusz
Journal: Medical science monitor : international medical journal of experimental and clinical research (2003): BR66-9
Fluorescent dyes modify properties of proteins used in microvascular research.
Authors: Bingaman, Susan and Huxley, Virginia H and Rumbaut, Rolando E
Journal: Microcirculation (New York, N.Y. : 1994) (2003): 221-31
Authors: Bingaman, Susan and Huxley, Virginia H and Rumbaut, Rolando E
Journal: Microcirculation (New York, N.Y. : 1994) (2003): 221-31
Application notes
A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
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