Name: iFluor® 532 succinimidyl ester
Brand: AAT Bioquest
SKU: 1025
Availability: InStock

iFluor® 532 succinimidyl ester

iFluor® 532 Succinimidyl Ester is an amine-reactive fluorescent dye for covalent labeling of antibodies and other proteins in fluorescence imaging and flow cytometry applications.

Amine-Reactive Functionality: NHS ester selectively reacts with primary amines, including lysine residues.
Spectral Properties: Excitation at 537 nm and emission at 560 nm.
Fluorescence Performance: Bright, photostable fluorescence with a quantum yield of 0.68.
Applications: Suitable for fluorescent conjugation of antibodies and other amine-containing proteins.

<p>HeLa cells were stained with mouse anti-tubulin followed with iFluor<sup>TM</sup> 532 goat anti-mouse IgG (H+L). Cell nuclei were stained with DAPI (Cat#17507).</p>

Protocol

Catalog	Size	Price
1025	1 mg	Price
71025	100 ug	Price
71505	5 mg	Price
71555	10 mg	Price

Overview

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, and 633 nm). iFluor® 532 dyes have fluorescence excitation and emission maxima of ~537 nm and ~560 nm respectively. These spectral characteristics make them an excellent upgrade to Alexa Fluor® 532 labeling dye (Alexa Fluor® is the trademark of Invitrogen). iFluor® 532 is perfectly excited at 532 nm with its maximum excitation at 537 nm, while Alexa Fluor® 532 has its maximum excitation at 524 nm (~8 nm off the desired 532 nm laser line). iFluor® 532 has a fluorescence quantum yield higher than Alexa Fluor® 532 by ~40% under the same conditions. In addition, iFluor® 532 is much more stable at a high pH range (e.g., 8-10, commonly used in DNA sequencing) than Alexa Fluor® 532. iFluor® 532 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 532 succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	109.402 µL	547.01 µL	1.094 mL	5.47 mL	10.94 mL
5 mM	21.88 µL	109.402 µL	218.804 µL	1.094 mL	2.188 mL
10 mM	10.94 µL	54.701 µL	109.402 µL	547.01 µL	1.094 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	914.06 g/mol	=	mL	x	mM	=	mmol

Citations

View all 3 citations: Citation Explorer

Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification

Authors:

Gao, D and Liu, N and Li, Y and Zhang, Y and Liu, G and others, undefined

Journal:

Metabolomics (Los Angel) (2017): 2153--0769

Suramin inhibits cullin-RING E3 ubiquitin ligases

Authors:

Wu, Kenneth and Chong, Robert A and Yu, Qing and Bai, Jin and Spratt, Donald E and Ching, Kevin and Lee, Chan and Miao, Haibin and Tappin, Inger and Hurwitz, Jerard and others, undefined

Journal:

Proceedings of the National Academy of Sciences (2016): E2011--E2018

Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function

Authors:

Piccard, Helene and Berghmans, Nele and Korpos, Eva and Dillen, Chris and Aelst, Ilse Van and Li, S and ra , undefined and Martens, Erik and Liekens, S and ra , undefined and Noppen, Sam and Damme, Jo Van and others, undefined

Journal:

International Journal of Cancer (2012): E425--E436

References

View all 49 references: Citation Explorer

Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes

Authors:

Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.

Journal:

Mol Biosyst (2011): 1766

Visualizing dengue virus through Alexa Fluor labeling

Authors:

Zhang S, Tan HC, Ooi EE.

Journal:

J Vis Exp. (2011)

Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells

Authors:

Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.

Journal:

Biochem Biophys Res Commun (2011): 211

Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594

Authors:

Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.

Journal:

Zhen Ci Yan Jiu (2011): 262

Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers

Authors:

Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.

Journal:

J Microbiol Methods (2011): 119

Physical properties

Molecular weight	914.06
Solvent	DMSO

Spectral properties

Absorbance (nm)	536
Correction factor (260 nm)	0.26
Correction factor (280 nm)	0.16
Extinction coefficient (cm ^-1 M ^-1)	90000 ¹
Excitation (nm)	537
Emission (nm)	560
Quantum yield	0.68 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on October 31, 2026