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iFluor® 532 succinimidyl ester
AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, and 633 nm). iFluor® 532 dyes have fluorescence excitation and emission maxima of ~537 nm and ~560 nm respectively. These spectral characteristics make them an excellent upgrade to Alexa Fluor® 532 labeling dye (Alexa Fluor® is the trademark of Invitrogen). iFluor® 532 is perfectly excited at 532 nm with its maximum excitation at 537 nm, while Alexa Fluor® 532 has its maximum excitation at 524 nm (~8 nm off the desired 532 nm laser line). iFluor® 532 has a fluorescence quantum yield higher than Alexa Fluor® 532 by ~40% under the same conditions. In addition, iFluor® 532 is much more stable at a high pH range (e.g., 8-10, commonly used in DNA sequencing) than Alexa Fluor® 532. iFluor® 532 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups.
<p>HeLa cells were stained with mouse anti-tubulin followed with iFluor<sup>TM</sup> 532 goat anti-mouse IgG (H+L). Cell nuclei were stained with DAPI (Cat#17507).</p>
<p>HeLa cells were stained with mouse anti-tubulin followed with iFluor<sup>TM</sup> 532 goat anti-mouse IgG (H+L). Cell nuclei were stained with DAPI (Cat#17507).</p>
CatalogSize
Price
Quantity
10251 mg
Price
71025100 ug
Price
715055 mg
Price
7155510 mg
Price
 
Physical properties

Molecular weight914.06
SolventDMSO
Spectral properties

Absorbance (nm)536
Correction factor (260 nm)0.26
Correction factor (280 nm)0.16
Extinction coefficient (cm -1 M -1)
90000
1
Excitation (nm)537
Emission (nm)560
Quantum yield
0.68
1
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Page updated on October 8, 2025