Name: iFluor® 510 succinimidyl ester
Brand: AAT Bioquest
SKU: 1424
Price: 237 USD
Availability: InStock

iFluor® 510 succinimidyl ester

iFluor® 510 Succinimidyl Ester is a green-fluorescent, amine-reactive dye for covalent labeling of antibodies and other proteins in fluorescence-based research applications.

Amine-Reactive Functionality: NHS ester selectively reacts with primary amines, including lysine residues.
Spectral Properties: Excitation at 511 nm and emission at 530 nm.
Fluorescence Performance: Bright and photostable fluorescence.
Instrument Compatibility: Supports blue-green excitation with 488–514 nm laser sources.
Applications: Suitable for fluorescent conjugation of antibodies and other amine-containing proteins.

HeLa cells were incubated with (+Tubulin) or without (control) mouse anti-tubulin followed by iFluor® 510 goat anti-mouse IgG conjugate. Cells were imaged using a fluorescence microscope equipped with a FITC filter set.

Protocol

SDS

Catalog	Size	Price	Quantity
1424	1 mg	Price

Overview

iFluor® 510 succinimidyl ester is the most convenient form of activated iFluor® 510 derivatives that can be used for labeling proteins such as antibodies. It is a new fluorescent dye belonging to the iFluor® family of dyes. These dyes are known for their bright fluorescence, photostability, and compatibility with various imaging techniques and instruments. iFluor® 510 emits green fluorescence when excited with light in the blue to green range (around 488 to 514 nm). Its emission peak is typically around 520 to 530 nm. The number "510" in its name represents the approximate maximum excitation wavelength. Like other iFluor® dyes, iFluor® 510 is commonly used to develop a variety of conjugates in biological research and imaging applications. It can be conjugated to various biomolecules, such as antibodies, proteins, nucleic acids, and small molecules, for fluorescence microscopy, flow cytometry, immunohistochemistry, and other fluorescence-based assays. The iFluor® dyes are designed to offer enhanced performance compared to traditional dyes, with features like high quantum yields, excellent brightness, minimal background noise, and resistance to photobleaching. iFluor® 510, specifically, is suitable for applications where green fluorescence is desired.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 510 succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	105.052 µL	525.26 µL	1.051 mL	5.253 mL	10.505 mL
5 mM	21.01 µL	105.052 µL	210.104 µL	1.051 mL	2.101 mL
10 mM	10.505 µL	52.526 µL	105.052 µL	525.26 µL	1.051 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	951.91 g/mol	=	mL	x	mM	=	mmol

References

View all 50 references: Citation Explorer

A dual-mode optical fiber sensor for SERS and fluorescence detection in liquid.

Authors:

Li, Minglu and Yan, Mingming and Xu, Ben and Zhao, Chunliu and Wang, Dongning and Wang, Yi and Chen, Huifang

Journal:

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2023): 122267

Toward Plasmonic Neural Probes: SERS Detection of Neurotransmitters through Gold-Nanoislands-Decorated Tapered Optical Fibers with Sub-10 nm Gaps.

Authors:

Zheng, Di and Pisano, Filippo and Collard, Liam and Balena, Antonio and Pisanello, Marco and Spagnolo, Barbara and Mach-Batlle, Rosa and Tantussi, Francesco and Carbone, Luigi and De Angelis, Francesco and Valiente, Manuel and de la Prida, Liset M and Ciracì, Cristian and De Vittorio, Massimo and Pisanello, Ferruccio

Journal:

Advanced materials (Deerfield Beach, Fla.) (2023): e2200902

Label-free DNA detection using silver nanoprism decorated silicon nanoparticles: Effect of silicon nanoparticle size and doping levels.

Authors:

Daoudi, Kais and Columbus, Soumya and Falcão, Bruno P and Pereira, Rui N and Peripolli, Suzana B and Ramachandran, Krithikadevi and Hadj Kacem, Hassen and Allagui, Anis and Gaidi, Mounir

Journal:

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2023): 122262

Manifold Learning Enables Interpretable Analysis of Raman Spectra from Extracellular Vesicle and Other Mixtures.

Authors:

Kazemzadeh, Mohammadrahim and Martinez-Calderon, Miguel and Otupiri, Robert and Artuyants, Anastasiia and Lowe, Moi M and Ning, Xia and Reategui, Eduardo and Schultz, Zachary D and Xu, Weiliang and Blenkiron, Cherie and Chamley, Lawrence W and Broderick, Neil G R and Hisey, Colin L

Journal:

bioRxiv : the preprint server for biology (2023)

Toward SERS based localized thermometry of Polymer-Supported silver and gold nanostructures.

Authors:

Prezgot, Daniel and Cruikshank, Jack and Makila-Boivin, Merrick and Birgani, Saro and Ianoul, Anatoli

Journal:

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2022): 121514

Physical properties

Molecular weight	951.91
Solvent	DMSO

Spectral properties

Excitation (nm)	511
Emission (nm)	530

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on October 25, 2026