Name: iFluor® 450 succinimidyl ester
Brand: AAT Bioquest
SKU: 1026
Availability: InStock

iFluor® 450 succinimidyl ester

iFluor® 450 Succinimidyl Ester is an amine-reactive fluorescent dye for covalent labeling of antibodies and other proteins in fluorescence imaging and flow cytometry applications.

Amine-Reactive Functionality: NHS ester selectively reacts with primary amines, including lysine residues.
Spectral Properties: Excitation at 451 nm and emission at 502 nm.
Fluorescence Performance: Bright, photostable fluorescence with minimal quenching.
Multicolor Compatibility: Provides an additional fluorescence color between Alexa Fluor® 430 and 488.
Applications: Suitable for labeling proteins and antibodies for fluorescence imaging and flow cytometry.

<p>HL-60 cells were incubated with (Red, +) or without (Green, -) Anti-human HLA-ABC (W6/32 mAb), followed by iFluor® 450 goat anti-mouse IgG conjugate. The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in FITC channel.</p>

Protocol

Catalog	Size	Price
1026	1 mg	Price
71026	100 ug	Price
71506	5 mg	Price
71556	10 mg	Price

Overview

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, and 633 nm). iFluor® 450 dyes have fluorescence excitation and emission maxima of ~451 nm and ~502 nm respectively. These spectral characteristics make them an excellent choice for multicolor applications. iFluor® 450 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups. iFluor® 450 SE provides a convenient tool to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) for fluorescence imaging and flow cytometric applications. iFluor® 450 dye conjugates are bright, providing an additional color between Alexa Fluor® 430 and 488 (ThermoFisher).

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 450 succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	165.648 µL	828.24 µL	1.656 mL	8.282 mL	16.565 mL
5 mM	33.13 µL	165.648 µL	331.296 µL	1.656 mL	3.313 mL
10 mM	16.565 µL	82.824 µL	165.648 µL	828.24 µL	1.656 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	603.69 g/mol	=	mL	x	mM	=	mmol

Citations

View all 4 citations: Citation Explorer

Lineage-enriched cell proliferation code underlies spatio-temporal regulation of cell cycle dynamics in lymphatic vessels

Authors:

Koltowska, Katarzyna and Gloger, Marleen and Kraft, Marle and Peng, Di and Carlantoni, Claudia and Skoczylas, Renae and Johansson, Anna and Smialowska, Agata and Makinen, Taija and Frye, Maike

Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification

Authors:

Gao, D and Liu, N and Li, Y and Zhang, Y and Liu, G and others, undefined

Journal:

Metabolomics (Los Angel) (2017): 2153--0769

Suramin inhibits cullin-RING E3 ubiquitin ligases

Authors:

Wu, Kenneth and Chong, Robert A and Yu, Qing and Bai, Jin and Spratt, Donald E and Ching, Kevin and Lee, Chan and Miao, Haibin and Tappin, Inger and Hurwitz, Jerard and others, undefined

Journal:

Proceedings of the National Academy of Sciences (2016): E2011--E2018

Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function

Authors:

Piccard, Helene and Berghmans, Nele and Korpos, Eva and Dillen, Chris and Aelst, Ilse Van and Li, S and ra , undefined and Martens, Erik and Liekens, S and ra , undefined and Noppen, Sam and Damme, Jo Van and others, undefined

Journal:

International Journal of Cancer (2012): E425--E436

References

View all 49 references: Citation Explorer

Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes

Authors:

Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.

Journal:

Mol Biosyst (2011): 1766

Visualizing dengue virus through Alexa Fluor labeling

Authors:

Zhang S, Tan HC, Ooi EE.

Journal:

J Vis Exp. (2011)

Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells

Authors:

Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.

Journal:

Biochem Biophys Res Commun (2011): 211

Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594

Authors:

Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.

Journal:

Zhen Ci Yan Jiu (2011): 262

Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers

Authors:

Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.

Journal:

J Microbiol Methods (2011): 119

Physical properties

Molecular weight	603.69
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.45
Correction factor (280 nm)	0.27
Extinction coefficient (cm ^-1 M ^-1)	40000 ¹
Excitation (nm)	451
Emission (nm)	502
Quantum yield	0.82 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 23, 2026