iFluor® 670 maleimide
Ordering information
Price | |
Catalog Number | |
Unit Size | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
Quotation | Request |
International | See distributors |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 971.03 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.03 |
Correction Factor (280 nm) | 0.033 |
Extinction coefficient (cm -1 M -1) | 2000001 |
Excitation (nm) | 671 |
Emission (nm) | 682 |
Quantum yield | 0.551 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
iFluor® 670 succinimidyl ester |
Overview | ![]() ![]() |
Molecular weight 971.03 | Correction Factor (260 nm) 0.03 | Correction Factor (280 nm) 0.033 | Extinction coefficient (cm -1 M -1) 2000001 | Excitation (nm) 671 | Emission (nm) 682 | Quantum yield 0.551 |
AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 514, 555, 633, 647 and 808 nm). iFluor® 670 dyes have fluorescence excitation and emission maxima of ~670 nm and ~690 nm respectively. These spectral characteristics make them a unique acceptor for preparing PE, APC and PerCP tandems that are widely used in flow cytometry applications. iFluor® 670 dyes have excellent photostability. iFluor® 670 Maleimide is stable and shows good reactivity and selectivity with protein thiol group.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 670 maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 102.983 µL | 514.917 µL | 1.03 mL | 5.149 mL | 10.298 mL |
5 mM | 20.597 µL | 102.983 µL | 205.967 µL | 1.03 mL | 2.06 mL |
10 mM | 10.298 µL | 51.492 µL | 102.983 µL | 514.917 µL | 1.03 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer


Spectral properties
Correction Factor (260 nm) | 0.03 |
Correction Factor (280 nm) | 0.033 |
Extinction coefficient (cm -1 M -1) | 2000001 |
Excitation (nm) | 671 |
Emission (nm) | 682 |
Quantum yield | 0.551 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
iFluor® 350 maleimide | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
iFluor® 488 maleimide | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 555 maleimide | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
iFluor® 647 maleimide | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 |
iFluor® 680 maleimide | 684 | 701 | 2200001 | 0.231 | 0.097 | 0.094 |
iFluor® 700 maleimide | 690 | 713 | 2200001 | 0.231 | 0.09 | 0.04 |
iFluor® 750 maleimide | 757 | 779 | 2750001 | 0.121 | 0.044 | 0.039 |
iFluor® 790 maleimide | 787 | 812 | 2500001 | 0.131 | 0.1 | 0.09 |
iFluor® 800 maleimide | 801 | 820 | 2500001 | 0.111 | 0.03 | 0.08 |
Show More (19) |
Images

Figure 1. iFluor® 670 dyes have fluorescence excitation and emission maxima of ~670 nm and ~690 nm respectively. These spectral characteristics make them a unique acceptor for preparing PE, APC and PerCP tandems that are widely used in flow cytometry applications. iFluor® 670 dyes have excellent photostability. iFluor® 670 Maleimide is stable and shows good reactivity and selectivity with protein thiol group.
References
View all 24 references: Citation Explorer
Development of fluorescence-based nucleic acid blot hybridization method using Cy5.5 labeled DNA probes.
Authors: Cheng, Ying and Wang, Na and Ren, Zhenxing and Xu, Chenggang
Journal: Journal of microbiological methods (2022): 106479
Authors: Cheng, Ying and Wang, Na and Ren, Zhenxing and Xu, Chenggang
Journal: Journal of microbiological methods (2022): 106479
Spurious CD34 expression in B-cell lymphoma due to nonspecific binding to PerCP-Cy5.5 fluorochrome conjugates: A rare phenomenon and a diagnostic pitfall.
Authors: Shi, Min and Timm, Michael M and Howard, Matthew T and Jevremovic, Dragan and Yuan, Ji and Greipp, Patricia T and Peterson, Jess F and Roh, Dana J and Horna, Pedro and Olteanu, Horatiu
Journal: Cytometry. Part B, Clinical cytometry (2022)
Authors: Shi, Min and Timm, Michael M and Howard, Matthew T and Jevremovic, Dragan and Yuan, Ji and Greipp, Patricia T and Peterson, Jess F and Roh, Dana J and Horna, Pedro and Olteanu, Horatiu
Journal: Cytometry. Part B, Clinical cytometry (2022)
Synthesis and characterization of 64Cu- and Cy5.5-labeled tetraiodothyroacetic acid derivatives for tumor angiogenesis imaging.
Authors: Kim, Hyunjung and Koo, Hyun-Jung and Ahn, Jinhee and Kim, Jung Young and Choi, Joon Young and Lee, Kyung-Han and Kim, Byung-Tae and Choe, Yearn Seong
Journal: Bioorganic & medicinal chemistry (2020): 115212
Authors: Kim, Hyunjung and Koo, Hyun-Jung and Ahn, Jinhee and Kim, Jung Young and Choi, Joon Young and Lee, Kyung-Han and Kim, Byung-Tae and Choe, Yearn Seong
Journal: Bioorganic & medicinal chemistry (2020): 115212
pH responsible and fluorescent Cy5.5-PEG-g-A-HA/CDDP complex nanoparticles: synthesis, characterization, and application for targeted drug delivery.
Authors: Cheng, Cui and Meng, Yabin and Zhang, Zhihong and Li, Ya and Liu, Chun and Zhang, Qiqing
Journal: Journal of materials science. Materials in medicine (2019): 58
Authors: Cheng, Cui and Meng, Yabin and Zhang, Zhihong and Li, Ya and Liu, Chun and Zhang, Qiqing
Journal: Journal of materials science. Materials in medicine (2019): 58
In Vivo Spinal Distribution of Cy5.5 Fluorescent Dye after Injection via the Lateral Ventricle and Cisterna Magna in Rat Model.
Authors: Lee, Kee-Hang and Nam, Hyun and Won, Jeong-Seob and Hwang, Ji-Yoon and Jang, Hye Won and Lee, Sun-Ho and Joo, Kyeung Min
Journal: Journal of Korean Neurosurgical Society (2018): 434-440
Authors: Lee, Kee-Hang and Nam, Hyun and Won, Jeong-Seob and Hwang, Ji-Yoon and Jang, Hye Won and Lee, Sun-Ho and Joo, Kyeung Min
Journal: Journal of Korean Neurosurgical Society (2018): 434-440
Tumoral Acidic pH-Responsive cis-Diaminodichloroplatinum-Incorporated Cy5.5-PEG- g-A-HA Nanoparticles for Targeting Delivery of CDDP against Cervical Cancer.
Authors: Cheng, Cui and Meng, Yabin and Zhang, Zhihong and Li, Ya and Zhang, Qiqing
Journal: ACS applied materials & interfaces (2018): 26882-26892
Authors: Cheng, Cui and Meng, Yabin and Zhang, Zhihong and Li, Ya and Zhang, Qiqing
Journal: ACS applied materials & interfaces (2018): 26882-26892
Synthesis and evaluation of Cy5.5-Rit tracer for specific near-infrared fluorescence imaging of sentinel lymph node.
Authors: Li, Nan and Zhu, Hua and Li, Yan and Wang, Jing and Yang, Zhi
Journal: Bioorganic & medicinal chemistry letters (2016): 4233-6
Authors: Li, Nan and Zhu, Hua and Li, Yan and Wang, Jing and Yang, Zhi
Journal: Bioorganic & medicinal chemistry letters (2016): 4233-6
In vivo quantifying molecular specificity of Cy5.5-labeled cyclic 9-mer peptide probe with dynamic fluorescence imaging.
Authors: Dai, Yunpeng and Yin, Jipeng and Huang, Yu and Chen, Xueli and Wang, Guodong and Liu, Yajun and Zhang, Xianghan and Nie, Yongzhan and Wu, Kaichun and Liang, Jimin
Journal: Biomedical optics express (2016): 1149-59
Authors: Dai, Yunpeng and Yin, Jipeng and Huang, Yu and Chen, Xueli and Wang, Guodong and Liu, Yajun and Zhang, Xianghan and Nie, Yongzhan and Wu, Kaichun and Liang, Jimin
Journal: Biomedical optics express (2016): 1149-59
Investigation of injection dose and camera integration time on quantifying pharmacokinetics of a Cy5.5-GX1 probe with dynamic fluorescence imaging in vivo.
Authors: Dai, Yunpeng and Chen, Xueli and Yin, Jipeng and Kang, Xiaoyu and Wang, Guodong and Zhang, Xianghan and Nie, Yongzhan and Wu, Kaichun and Liang, Jimin
Journal: Journal of biomedical optics (2016): 86001
Authors: Dai, Yunpeng and Chen, Xueli and Yin, Jipeng and Kang, Xiaoyu and Wang, Guodong and Zhang, Xianghan and Nie, Yongzhan and Wu, Kaichun and Liang, Jimin
Journal: Journal of biomedical optics (2016): 86001
Catabolism of (64)Cu and Cy5.5-labeled human serum albumin in a tumor xenograft model.
Authors: Kang, Choong Mo and Kim, Hyunjung and Koo, Hyun-Jung and Park, Jin Won and An, Gwang Il and Choi, Joon Young and Lee, Kyung-Han and Kim, Byung-Tae and Choe, Yearn Seong
Journal: Amino acids (2016): 1667-75
Authors: Kang, Choong Mo and Kim, Hyunjung and Koo, Hyun-Jung and Park, Jin Won and An, Gwang Il and Choi, Joon Young and Lee, Kyung-Han and Kim, Byung-Tae and Choe, Yearn Seong
Journal: Amino acids (2016): 1667-75