iFluor® 670 maleimide

iFluor® 670 dyes have fluorescence excitation and emission maxima of ~670 nm and ~690 nm respectively. These spectral characteristics make them a unique acceptor for preparing PE, APC and PerCP tandems that are widely used in flow cytometry applications. iFluor® 670 dyes have excellent photostability. iFluor® 670 Maleimide is stable and shows good reactivity and selectivity with protein thiol group.

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	971.03
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.03
Correction Factor (280 nm)	0.033
Extinction coefficient (cm ^-1 M ^-1)	200000¹
Excitation (nm)	671
Emission (nm)	682
Quantum yield	0.55¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Alternative formats

iFluor® 670 succinimidyl ester

Overview SDS Protocol

Molecular weight

971.03

Correction Factor (260 nm)

0.03

Correction Factor (280 nm)

0.033

Extinction coefficient (cm ^-1 M ^-1)

200000¹

Excitation (nm)

671

Emission (nm)

682

Quantum yield

0.55¹

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 514, 555, 633, 647 and 808 nm). iFluor® 670 dyes have fluorescence excitation and emission maxima of ~670 nm and ~690 nm respectively. These spectral characteristics make them a unique acceptor for preparing PE, APC and PerCP tandems that are widely used in flow cytometry applications. iFluor® 670 dyes have excellent photostability. iFluor® 670 Maleimide is stable and shows good reactivity and selectivity with protein thiol group.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 670 maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	102.983 µL	514.917 µL	1.03 mL	5.149 mL	10.298 mL
5 mM	20.597 µL	102.983 µL	205.967 µL	1.03 mL	2.06 mL
10 mM	10.298 µL	51.492 µL	102.983 µL	514.917 µL	1.03 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Correction Factor (260 nm)	0.03
Correction Factor (280 nm)	0.033
Extinction coefficient (cm ^-1 M ^-1)	200000¹
Excitation (nm)	671
Emission (nm)	682
Quantum yield	0.55¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
iFluor® 350 maleimide	345	450	20000¹	0.95¹	0.83	0.23
iFluor® 488 maleimide	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 555 maleimide	557	570	100000¹	0.64¹	0.23	0.14
iFluor® 647 maleimide	656	670	250000¹	0.25¹	0.03	0.03
iFluor® 680 maleimide	684	701	220000¹	0.23¹	0.097	0.094
iFluor® 700 maleimide	690	713	220000¹	0.23¹	0.09	0.04
iFluor® 750 maleimide	757	779	275000¹	0.12¹	0.044	0.039
iFluor® 790 maleimide	787	812	250000¹	0.13¹	0.1	0.09
iFluor® 800 maleimide	801	820	250000¹	0.11¹	0.03	0.08
iFluor® 810 maleimide	811	822	250000¹	0.05¹	0.09	0.15
iFluor® 820 maleimide	822	850	250000¹		0.11	0.16
iFluor® 860 maleimide	853	878	250000¹		0.1	0.14
iFluor® 532 maleimide	537	560	90000¹	0.68¹	0.26	0.16
iFluor® 594 maleimide	588	604	180000¹	0.53¹	0.05	0.04
iFluor® 405 maleimide	403	427	37000¹	0.91¹	0.48	0.77
iFluor® 430 maleimide	433	498	40000¹	0.78¹	0.68	0.3
iFluor® 568 maleimide	568	587	100000¹	0.57¹	0.34	0.15
iFluor® 633 maleimide	640	654	250000¹	0.29¹	0.062	0.044
iFluor® 450 maleimide	451	502	40000¹	0.82¹	0.45	0.27
iFluor® 460 maleimide	468	493	80000¹	~0.8¹	0.98	0.46
iFluor® 665 maleimide	667	692	110,000¹	0.22¹	0.12	0.09
iFluor® 546 maleimide	541	557	100000¹	0.67¹	0.25	0.15
iFluor® 840 maleimide	836	879	200000¹	-	0.2	0.09
iFluor® 770 maleimide	777	797	250000¹	0.16	0.09	0.08
iFluor® 780 maleimide	784	808	250000¹	0.16¹	0.13	0.12
iFluor® 830 maleimide	830	867	-	-	-	-
iFluor® 514 maleimide	511	527	75000¹	0.83¹	0.265	0.116
iFluor® 660 maleimide	663	678	250000¹	0.26¹	0.07	0.08
Show More (19)

Images

Figure 1. iFluor® 670 dyes have fluorescence excitation and emission maxima of ~670 nm and ~690 nm respectively. These spectral characteristics make them a unique acceptor for preparing PE, APC and PerCP tandems that are widely used in flow cytometry applications. iFluor® 670 dyes have excellent photostability. iFluor® 670 Maleimide is stable and shows good reactivity and selectivity with protein thiol group.

References

View all 24 references: Citation Explorer

Development of fluorescence-based nucleic acid blot hybridization method using Cy5.5 labeled DNA probes.
Authors: Cheng, Ying and Wang, Na and Ren, Zhenxing and Xu, Chenggang
Journal: Journal of microbiological methods (2022): 106479

Spurious CD34 expression in B-cell lymphoma due to nonspecific binding to PerCP-Cy5.5 fluorochrome conjugates: A rare phenomenon and a diagnostic pitfall.
Authors: Shi, Min and Timm, Michael M and Howard, Matthew T and Jevremovic, Dragan and Yuan, Ji and Greipp, Patricia T and Peterson, Jess F and Roh, Dana J and Horna, Pedro and Olteanu, Horatiu
Journal: Cytometry. Part B, Clinical cytometry (2022)

Synthesis and characterization of 64Cu- and Cy5.5-labeled tetraiodothyroacetic acid derivatives for tumor angiogenesis imaging.
Authors: Kim, Hyunjung and Koo, Hyun-Jung and Ahn, Jinhee and Kim, Jung Young and Choi, Joon Young and Lee, Kyung-Han and Kim, Byung-Tae and Choe, Yearn Seong
Journal: Bioorganic & medicinal chemistry (2020): 115212

pH responsible and fluorescent Cy5.5-PEG-g-A-HA/CDDP complex nanoparticles: synthesis, characterization, and application for targeted drug delivery.
Authors: Cheng, Cui and Meng, Yabin and Zhang, Zhihong and Li, Ya and Liu, Chun and Zhang, Qiqing
Journal: Journal of materials science. Materials in medicine (2019): 58

In Vivo Spinal Distribution of Cy5.5 Fluorescent Dye after Injection via the Lateral Ventricle and Cisterna Magna in Rat Model.
Authors: Lee, Kee-Hang and Nam, Hyun and Won, Jeong-Seob and Hwang, Ji-Yoon and Jang, Hye Won and Lee, Sun-Ho and Joo, Kyeung Min
Journal: Journal of Korean Neurosurgical Society (2018): 434-440

Tumoral Acidic pH-Responsive cis-Diaminodichloroplatinum-Incorporated Cy5.5-PEG- g-A-HA Nanoparticles for Targeting Delivery of CDDP against Cervical Cancer.
Authors: Cheng, Cui and Meng, Yabin and Zhang, Zhihong and Li, Ya and Zhang, Qiqing
Journal: ACS applied materials & interfaces (2018): 26882-26892

Synthesis and evaluation of Cy5.5-Rit tracer for specific near-infrared fluorescence imaging of sentinel lymph node.
Authors: Li, Nan and Zhu, Hua and Li, Yan and Wang, Jing and Yang, Zhi
Journal: Bioorganic & medicinal chemistry letters (2016): 4233-6

In vivo quantifying molecular specificity of Cy5.5-labeled cyclic 9-mer peptide probe with dynamic fluorescence imaging.
Authors: Dai, Yunpeng and Yin, Jipeng and Huang, Yu and Chen, Xueli and Wang, Guodong and Liu, Yajun and Zhang, Xianghan and Nie, Yongzhan and Wu, Kaichun and Liang, Jimin
Journal: Biomedical optics express (2016): 1149-59

Investigation of injection dose and camera integration time on quantifying pharmacokinetics of a Cy5.5-GX1 probe with dynamic fluorescence imaging in vivo.
Authors: Dai, Yunpeng and Chen, Xueli and Yin, Jipeng and Kang, Xiaoyu and Wang, Guodong and Zhang, Xianghan and Nie, Yongzhan and Wu, Kaichun and Liang, Jimin
Journal: Journal of biomedical optics (2016): 86001

Catabolism of (64)Cu and Cy5.5-labeled human serum albumin in a tumor xenograft model.
Authors: Kang, Choong Mo and Kim, Hyunjung and Koo, Hyun-Jung and Park, Jin Won and An, Gwang Il and Choi, Joon Young and Lee, Kyung-Han and Kim, Byung-Tae and Choe, Yearn Seong
Journal: Amino acids (2016): 1667-75

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iFluor® 670 maleimide

OverviewSDSProtocol

Calculators

Common stock solution preparation

Molarity calculator

Spectrum

Spectral properties

Product Family

Images

References

Documents

Overview SDS Protocol