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iFluor® 670 dyes have fluorescence excitation and emission maxima of ~670 nm and ~690 nm respectively. These spectral characteristics make them a unique acceptor for preparing PE, APC and PerCP tandems that are widely used in flow cytometry applications. iFluor® 670 dyes have excellent photostability. iFluor® 670 Maleimide is stable and shows good reactivity and selectivity with protein thiol group.
iFluor® 670 dyes have fluorescence excitation and emission maxima of ~670 nm and ~690 nm respectively. These spectral characteristics make them a unique acceptor for preparing PE, APC and PerCP tandems that are widely used in flow cytometry applications. iFluor® 670 dyes have excellent photostability. iFluor® 670 Maleimide is stable and shows good reactivity and selectivity with protein thiol group.
Ordering information
Price ()
Catalog Number72502
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight971.03
SolventDMSO
Spectral properties
Correction Factor (260 nm)0.03
Correction Factor (280 nm)0.033
Extinction coefficient (cm -1 M -1)2000001
Excitation (nm)671
Emission (nm)682
Quantum yield0.551
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
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iFluor® 488 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 514 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 532 goat anti-mouse IgG (H+L) *Cross Adsorbed*
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iFluor® 680 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 700 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 750 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 790 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 350 goat anti-rabbit IgG (H+L)
iFluor® 405 goat anti-rabbit IgG (H+L)
iFluor® 488 goat anti-rabbit IgG (H+L)
iFluor® 514 goat anti-rabbit IgG (H+L)
iFluor® 532 goat anti-rabbit IgG (H+L)
iFluor® 555 goat anti-rabbit IgG (H+L)
iFluor® 594 goat anti-rabbit IgG (H+L)
iFluor® 633 goat anti-rabbit IgG (H+L)
iFluor® 647 goat anti-rabbit IgG (H+L)
iFluor® 680 goat anti-rabbit IgG (H+L)
iFluor® 700 goat anti-rabbit IgG (H+L)
iFluor® 750 goat anti-rabbit IgG (H+L)
iFluor® 790 goat anti-rabbit IgG (H+L)
iFluor® 350 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 405 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 488 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 514 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 532 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 555 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 594 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 633 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
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iFluor® 350-streptavidin conjugate
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iFluor® 532-streptavidin conjugate
iFluor® 555-streptavidin conjugate
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iFluor® 750-streptavidin conjugate
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iFluor® 405 hydrazide
iFluor® 800 acid
iFluor® 810 acid
iFluor® 820 acid
iFluor® 860 acid
iFluor® 546 goat anti-mouse IgG (H+L)
iFluor® 546 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 568 goat anti-mouse IgG (H+L)
iFluor® 568 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 546 goat anti-rabbit IgG (H+L)
iFluor® 546 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 568 goat anti-rabbit IgG (H+L)
iFluor® 568 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
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iFluor® 568-streptavidin conjugate
iFluor® 488 alkyne
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iFluor® 488 Styramide *Superior Replacement for Alexa Fluor 488 tyramide and Opal 520*
iFluor® 546 Styramide *Superior Replacement for Alexa Fluor 546 tyramide*
iFluor® 555 Styramide *Superior Replacement for Alexa Fluor 555 tyramide and Opal 570*
iFluor® 568 Styramide *Superior Replacement for Alexa Fluor 568 tyramide*
iFluor® 594 Styramide *Superior Replacement for Alexa Fluor 594 tyramide*
iFluor® 647 Styramide *Superior Replacement for Alexa Fluor 647 tyramide*
iFluor® 680 Styramide *Superior Replacement for Alexa Fluor 680 tyramide and Opal 690*
iFluor® 700 Styramide *Superior Replacement for Alexa Fluor 700 tyramide*
iFluor® 750 Styramide *Superior Replacement for Alexa Fluor 750 tyramide*
iFluor® 790 Styramide *Superior Replacement for Alexa Fluor 790 tyramide*
iFluor® 555 Tyramide
iFluor® 647 Tyramide
iFluor® 350 PSA™ Imaging Kit with Goat Anti-Rabbit IgG
iFluor® 488 PSA™ Imaging Kit with Goat Anti-Rabbit IgG
iFluor® 555 PSA™ Imaging Kit with Goat Anti-Rabbit IgG
iFluor® 594 PSA™ Imaging Kit with Goat Anti-Rabbit IgG
iFluor® 647 PSA™ Imaging Kit with Goat Anti-Rabbit IgG
iFluor 350™ PSA™ Imaging Kit with Goat Anti-Mouse IgG
iFluor 488™ PSA™ Imaging Kit with Goat Anti-Mouse IgG
iFluor 555™ PSA™ Imaging Kit with Goat Anti-Mouse IgG
iFluor 594™ PSA™ Imaging Kit with Goat Anti-Mouse IgG
iFluor 647™ PSA™ Imaging Kit with Goat Anti-Mouse IgG
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iFluor® 790-streptavidin conjugate
iFluor® 800-streptavidin conjugate
iFluor® 820-streptavidin conjugate
iFluor® 840-streptavidin conjugate
iFluor® 860-streptavidin conjugate
iFluor® 488-Wheat Germ Agglutinin (WGA) Conjugate
iFluor® 555-Wheat Germ Agglutinin (WGA) Conjugate
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iFluor® 488-Concanavalin A Conjugate
iFluor® 555-Concanavalin A Conjugate
iFluor® 594-Concanavalin A Conjugate
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iFluor® 810 goat anti-mouse IgG (H+L)
iFluor® 810 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 820 goat anti-mouse IgG (H+L)
iFluor® 820 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 840 goat anti-mouse IgG (H+L)
iFluor® 840 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 860 goat anti-mouse IgG (H+L)
iFluor® 860 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 800 goat anti-rabbit IgG (H+L)
iFluor® 800 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 810 goat anti-rabbit IgG (H+L)
iFluor® 810 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 820 goat anti-rabbit IgG (H+L)
iFluor® 820 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 840 goat anti-rabbit IgG (H+L)
iFluor® 840 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 860 goat anti-rabbit IgG (H+L)
iFluor® 860 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
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iFluor® 450 Tyramide *Superior Replacement for Opal 480*
iFluor® 350 succinimidyl ester
iFluor® 405 succinimidyl ester
iFluor® 488 succinimidyl ester
iFluor® 514 succinimidyl ester
iFluor® 532 succinimidyl ester
iFluor® 555 succinimidyl ester
iFluor® 594 succinimidyl ester
iFluor® 633 succinimidyl ester
iFluor® 647 succinimidyl ester
iFluor® 660 succinimidyl ester
iFluor® 680 succinimidyl ester
iFluor® 700 succinimidyl ester
iFluor® 750 succinimidyl ester
iFluor® 610 succinimidyl ester
iFluor® 710 succinimidyl ester
iFluor® 790 succinimidyl ester
iFluor® 800 succinimidyl ester
iFluor® 810 succinimidyl ester
iFluor® 820 succinimidyl ester
iFluor® 860 succinimidyl ester
iFluor® 546 succinimidyl ester
iFluor® 568 succinimidyl ester
iFluor® 430 succinimidyl ester
iFluor® 450 succinimidyl ester
iFluor® 840 succinimidyl ester
iFluor® 560 succinimidyl ester
iFluor® 460 succinimidyl ester
iFluor® 440 succinimidyl ester
iFluor® 665 succinimidyl ester
iFluor® 690 succinimidyl ester
iFluor® Ultra 594 succinimidyl ester
iFluor® Ultra 647 succinimidyl ester
iFluor® Ultra 750 succinimidyl ester
iFluor® 720 succinimidyl ester
iFluor® 740 succinimidyl ester
iFluor® 597 succinimidyl ester
iFluor® 770 succinimidyl ester
iFluor® 780 succinimidyl ester
iFluor® 570 succinimidyl ester
iFluor® 830 acid
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iFluor™ 405 azide
Show More (236)

OverviewpdfSDSpdfProtocol


Molecular weight
971.03
Correction Factor (260 nm)
0.03
Correction Factor (280 nm)
0.033
Extinction coefficient (cm -1 M -1)
2000001
Excitation (nm)
671
Emission (nm)
682
Quantum yield
0.551
AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 514, 555, 633, 647 and 808 nm). iFluor® 670 dyes have fluorescence excitation and emission maxima of ~670 nm and ~690 nm respectively. These spectral characteristics make them a unique acceptor for preparing PE, APC and PerCP tandems that are widely used in flow cytometry applications. iFluor® 670 dyes have excellent photostability. iFluor® 670 Maleimide is stable and shows good reactivity and selectivity with protein thiol group.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 670 maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM102.983 µL514.917 µL1.03 mL5.149 mL10.298 mL
5 mM20.597 µL102.983 µL205.967 µL1.03 mL2.06 mL
10 mM10.298 µL51.492 µL102.983 µL514.917 µL1.03 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Correction Factor (260 nm)0.03
Correction Factor (280 nm)0.033
Extinction coefficient (cm -1 M -1)2000001
Excitation (nm)671
Emission (nm)682
Quantum yield0.551

Product family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
iFluor® 350 maleimide3454502000010.9510.830.23
iFluor® 488 maleimide4915167500010.910.210.11
iFluor® 555 maleimide55757010000010.6410.230.14
iFluor® 647 maleimide65667025000010.2510.030.03
iFluor® 680 maleimide68470122000010.2310.0970.094
iFluor® 700 maleimide69071322000010.2310.090.04
iFluor® 750 maleimide75777927500010.1210.0440.039
iFluor® 790 maleimide78781225000010.1310.10.09
iFluor® 800 maleimide80182025000010.1110.030.08
iFluor® 810 maleimide81182225000010.0510.090.15
iFluor® 820 maleimide8228502500001-0.110.16
iFluor® 860 maleimide8538782500001-0.10.14
iFluor® 532 maleimide5375609000010.6810.260.16
iFluor® 594 maleimide58860418000010.5310.050.04
iFluor® 405 maleimide4034273700010.9110.480.77
iFluor® 430 maleimide4334984000010.7810.680.3
iFluor® 568 maleimide56858710000010.5710.340.15
iFluor® 633 maleimide64065425000010.2910.0620.044
iFluor® 450 maleimide4515024000010.8210.450.27
iFluor® 460 maleimide468493800001~0.810.980.46
iFluor® 665 maleimide667692110,00010.2210.120.09
iFluor® 546 maleimide54155710000010.6710.250.15
iFluor® 840 maleimide8368792000001-0.20.09
iFluor® 770 maleimide77779725000010.160.090.08
iFluor® 780 maleimide78480825000010.1610.130.12
iFluor® 830 maleimide830867----
iFluor® 514 maleimide5115277500010.8310.2650.116
iFluor® 660 maleimide66367825000010.2610.070.08
Show More (29)

References


View all 24 references: Citation Explorer
Development of fluorescence-based nucleic acid blot hybridization method using Cy5.5 labeled DNA probes.
Authors: Cheng, Ying and Wang, Na and Ren, Zhenxing and Xu, Chenggang
Journal: Journal of microbiological methods (2022): 106479
Spurious CD34 expression in B-cell lymphoma due to nonspecific binding to PerCP-Cy5.5 fluorochrome conjugates: A rare phenomenon and a diagnostic pitfall.
Authors: Shi, Min and Timm, Michael M and Howard, Matthew T and Jevremovic, Dragan and Yuan, Ji and Greipp, Patricia T and Peterson, Jess F and Roh, Dana J and Horna, Pedro and Olteanu, Horatiu
Journal: Cytometry. Part B, Clinical cytometry (2022)
Synthesis and characterization of 64Cu- and Cy5.5-labeled tetraiodothyroacetic acid derivatives for tumor angiogenesis imaging.
Authors: Kim, Hyunjung and Koo, Hyun-Jung and Ahn, Jinhee and Kim, Jung Young and Choi, Joon Young and Lee, Kyung-Han and Kim, Byung-Tae and Choe, Yearn Seong
Journal: Bioorganic & medicinal chemistry (2020): 115212
pH responsible and fluorescent Cy5.5-PEG-g-A-HA/CDDP complex nanoparticles: synthesis, characterization, and application for targeted drug delivery.
Authors: Cheng, Cui and Meng, Yabin and Zhang, Zhihong and Li, Ya and Liu, Chun and Zhang, Qiqing
Journal: Journal of materials science. Materials in medicine (2019): 58
In Vivo Spinal Distribution of Cy5.5 Fluorescent Dye after Injection via the Lateral Ventricle and Cisterna Magna in Rat Model.
Authors: Lee, Kee-Hang and Nam, Hyun and Won, Jeong-Seob and Hwang, Ji-Yoon and Jang, Hye Won and Lee, Sun-Ho and Joo, Kyeung Min
Journal: Journal of Korean Neurosurgical Society (2018): 434-440
Tumoral Acidic pH-Responsive cis-Diaminodichloroplatinum-Incorporated Cy5.5-PEG- g-A-HA Nanoparticles for Targeting Delivery of CDDP against Cervical Cancer.
Authors: Cheng, Cui and Meng, Yabin and Zhang, Zhihong and Li, Ya and Zhang, Qiqing
Journal: ACS applied materials & interfaces (2018): 26882-26892
Synthesis and evaluation of Cy5.5-Rit tracer for specific near-infrared fluorescence imaging of sentinel lymph node.
Authors: Li, Nan and Zhu, Hua and Li, Yan and Wang, Jing and Yang, Zhi
Journal: Bioorganic & medicinal chemistry letters (2016): 4233-6
In vivo quantifying molecular specificity of Cy5.5-labeled cyclic 9-mer peptide probe with dynamic fluorescence imaging.
Authors: Dai, Yunpeng and Yin, Jipeng and Huang, Yu and Chen, Xueli and Wang, Guodong and Liu, Yajun and Zhang, Xianghan and Nie, Yongzhan and Wu, Kaichun and Liang, Jimin
Journal: Biomedical optics express (2016): 1149-59
Investigation of injection dose and camera integration time on quantifying pharmacokinetics of a Cy5.5-GX1 probe with dynamic fluorescence imaging in vivo.
Authors: Dai, Yunpeng and Chen, Xueli and Yin, Jipeng and Kang, Xiaoyu and Wang, Guodong and Zhang, Xianghan and Nie, Yongzhan and Wu, Kaichun and Liang, Jimin
Journal: Journal of biomedical optics (2016): 86001
Catabolism of (64)Cu and Cy5.5-labeled human serum albumin in a tumor xenograft model.
Authors: Kang, Choong Mo and Kim, Hyunjung and Koo, Hyun-Jung and Park, Jin Won and An, Gwang Il and Choi, Joon Young and Lee, Kyung-Han and Kim, Byung-Tae and Choe, Yearn Seong
Journal: Amino acids (2016): 1667-75