iFluor® 605 maleimide

Ordering information

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	928.09
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Alternative formats

Overview SDS Protocol

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 605 maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	107.748 µL	538.741 µL	1.077 mL	5.387 mL	10.775 mL
5 mM	21.55 µL	107.748 µL	215.496 µL	1.077 mL	2.155 mL
10 mM	10.775 µL	53.874 µL	107.748 µL	538.741 µL	1.077 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
iFluor® 350 maleimide	345	450	20000¹	0.95¹	0.83	0.23
iFluor® 488 maleimide	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 555 maleimide	557	570	100000¹	0.64¹	0.23	0.14
iFluor® 647 maleimide	656	670	250000¹	0.25¹	0.03	0.03
iFluor® 680 maleimide	684	701	220000¹	0.23¹	0.097	0.094
iFluor® 700 maleimide	690	713	220000¹	0.23¹	0.09	0.04
iFluor® 750 maleimide	757	779	275000¹	0.12¹	0.044	0.039
iFluor® 790 maleimide	787	812	250000¹	0.13¹	0.1	0.09
iFluor® 800 maleimide	801	820	250000¹	0.11¹	0.03	0.08
iFluor® 810 maleimide	811	822	250000¹	0.05¹	0.09	0.15
iFluor® 820 maleimide	822	850	250000¹		0.11	0.16
iFluor® 860 maleimide	853	878	250000¹		0.1	0.14
iFluor® 532 maleimide	537	560	90000¹	0.68¹	0.26	0.16
iFluor® 594 maleimide	588	604	180000¹	0.53¹	0.05	0.04
iFluor® 405 maleimide	403	427	37000¹	0.91¹	0.48	0.77
iFluor® 430 maleimide	433	498	40000¹	0.78¹	0.68	0.3
iFluor® 568 maleimide	568	587	100000¹	0.57¹	0.34	0.15
iFluor® 633 maleimide	640	654	250000¹	0.29¹	0.062	0.044
iFluor® 450 maleimide	451	502	40000¹	0.82¹	0.45	0.27
iFluor® 460 maleimide	468	493	80000¹	~0.8¹	0.98	0.46
iFluor® 665 maleimide	667	692	110,000¹	0.22¹	0.12	0.09
iFluor® 546 maleimide	541	557	100000¹	0.67¹	0.25	0.15
iFluor® 840 maleimide	836	879	200000¹	-	0.2	0.09
iFluor® 770 maleimide	777	797	250000¹	0.16	0.09	0.08
iFluor® 780 maleimide	784	808	250000¹	0.16¹	0.13	0.12
iFluor® 830 maleimide	830	867	-	-	-	-
iFluor® 514 maleimide	511	527	75000¹	0.83¹	0.265	0.116
iFluor® 660 maleimide	663	678	250000¹	0.26¹	0.07	0.08
iFluor® 670 maleimide	671	682	200000¹	0.55¹	0.03	0.033
Show More (20)

References

View all 40 references: Citation Explorer

Visualization of preimplantation uterine fluid absorption in mice using Alexa Fluor™ 488 hydrazide.
Authors: Li, Yuehuan and Martin, Taylor Elijah and Hancock, Jonathan Matthew and Li, Rong and Viswanathan, Suvitha and Lydon, John P and Zheng, Yi and Ye, Xiaoqin
Journal: Biology of reproduction (2022)

Alexa Fluor 488-conjugated cholera toxin subunit B optimally labels neurons 3-7 days after injection into the rat gastrocnemius muscle.
Authors: Cui, Jing-Jing and Wang, Jia and Xu, Dong-Sheng and Wu, Shuang and Guo, Ya-Ting and Su, Yu-Xin and Liu, Yi-Han and Wang, Yu-Qing and Jing, Xiang-Hong and Bai, Wan-Zhu
Journal: Neural regeneration research (2022): 2316-2320

Click-iT ® Plus OPP Alexa Fluor ® Protein Synthesis Assay in Embryonic Cells.
Authors: Li, Yan and Ji, Xu and Chang, Lu and Tang, Jianan and Hua, Min-Min and Liu, Jing and O'Neill, Christopher and Huang, Xuefeng and Jin, Xingliang
Journal: Bio-protocol (2022): e4441

Evaluation of Blood-Brain Barrier Integrity Using Vascular Permeability Markers: Evans Blue, Sodium Fluorescein, Albumin-Alexa Fluor Conjugates, and Horseradish Peroxidase.
Authors: Ahishali, Bulent and Kaya, Mehmet
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 87-103

Effects of Viscosity and Refractive Index on the Emission and Diffusion Properties of Alexa Fluor 405 Using Fluorescence Correlation and Lifetime Spectroscopies.
Authors: van Zanten, Camila and Melnikau, Dzmitry and Ryder, Alan G
Journal: Journal of fluorescence (2021): 835-845

Molecular and Spectroscopic Characterization of Green and Red Cyanine Fluorophores from the Alexa Fluor and AF Series*.
Authors: Gebhardt, Christian and Lehmann, Martin and Reif, Maria M and Zacharias, Martin and Gemmecker, Gerd and Cordes, Thorben
Journal: Chemphyschem : a European journal of chemical physics and physical chemistry (2021)

Molecular and Spectroscopic Characterization of Green and Red Cyanine Fluorophores from the Alexa Fluor and AF Series.
Authors: Gebhardt, Christian and Lehmann, Martin and Reif, Maria M and Zacharias, Martin and Gemmecker, Gerd and Cordes, Thorben
Journal: Chemphyschem : a European journal of chemical physics and physical chemistry (2021): 1546

Hot-Band Anti-Stokes Fluorescence Properties of Alexa Fluor 568.
Authors: Gajdos, Tamás and Hopp, Béla and Erdélyi, Miklós
Journal: Journal of fluorescence (2020): 437-443

Photo-isomerization of the Cyanine Dye Alexa-Fluor 647 (AF-647) in the Context of dSTORM Super-Resolution Microscopy.
Authors: Karlsson, Joshua K G and Laude, Alex and Hall, Michael J and Harriman, Anthony
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2019): 14983-14998

Observing the Reversible Single Molecule Electrochemistry of Alexa Fluor 647 Dyes by Total Internal Reflection Fluorescence Microscopy.
Authors: Fan, Sanjun and Webb, James E A and Yang, Ying and Nieves, Daniel J and Gonçales, Vinicius R and Tran, Jason and Hilzenrat, Geva and Kahram, Mohaddeseh and Tilley, Richard D and Gaus, Katharina and Gooding, J Justin
Journal: Angewandte Chemie (International ed. in English) (2019): 14495-14498