Name: iFluor® 460 maleimide
Brand: AAT Bioquest
SKU: 1058
Price: 226 USD
Availability: InStock

iFluor® 460 maleimide

iFluor® 460 Maleimide is a thiol-reactive fluorescent dye for selective covalent labeling of proteins, antibodies, and other thiol-containing biomolecules in fluorescence-based research applications.

Thiol-Reactive Functionality: Maleimide group selectively reacts with thiols, including cysteine residues.
Spectral Properties: Excitation at 468 nm and emission at 493 nm.
Fluorescence Performance: Bright, photostable fluorescence with minimal quenching.
Instrument Compatibility: Optimized for excitation with 460 nm blue diode lasers.
Applications: Suitable for fluorescent conjugation of proteins, antibodies, peptides, and thiol-modified oligonucleotides.

Fluorescent dye maleimides are the most popular tool for conjugating dyes to a peptide, protein, antibody, thiol-modified oligonucleotide or nucleic acid through their SH group. Maleimides react readily with the thiol group of proteins, thiol-modified oligonucleotides, and other thiol-containing molecules under neutral conditions. The resulting dye conjugates are quite stable.

Protocol

SDS

Catalog	Size	Price	Quantity
1058	1 mg	Price

Overview

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. Although the 460 nm blue diode laser is being installed in numerous new fluorescence instruments, few dyes can be well excited at 460 nm. iFluor® 460 is optimized to be well excited by the blue diode laser at 460 nm, enabling new biological applications for the new fluorescence instruments equipped with the 460 nm blue diode laser. iFluor® 460 maleimide is stable and shows good reactivity and selectivity with the thiol group.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 460 maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	120.21 µL	601.048 µL	1.202 mL	6.01 mL	12.021 mL
5 mM	24.042 µL	120.21 µL	240.419 µL	1.202 mL	2.404 mL
10 mM	12.021 µL	60.105 µL	120.21 µL	601.048 µL	1.202 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	831.88 g/mol	=	mL	x	mM	=	mmol

References

View all 50 references: Citation Explorer

Toward the Clinical Development and Validation of a Thy1-Targeted Ultrasound Contrast Agent for the Early Detection of Pancreatic Ductal Adenocarcinoma.

Authors:

Bam, Rakesh and Daryaei, Iman and Abou-Elkacem, Lotfi and Vilches-Moure, Jose G and Meuillet, Emmanuelle J and Lutz, Amelie and Marinelli, Edmund R and Unger, Evan C and Gambhir, Sanjiv S and Paulmurugan, Ramasamy

Journal:

Investigative radiology (2020)

Tracking the physical stability of fluorescent-labeled mAbs under physiologic in vitro conditions in human serum and PBS.

Authors:

Schuster, Joachim and Mahler, Hanns-Christian and Koulov, Atanas and Joerg, Susanne and Racher, Andy and Huwyler, Joerg and Detampel, Pascal and Mathaes, Roman

Journal:

European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenst (2020): 193-201

Folding of single-stranded circular DNA into rigid rectangular DNA accelerates its cellular uptake.

Authors:

Ohtsuki, Shozo and Shiba, Yukako and Maezawa, Tatsuoki and Hidaka, Kumi and Sugiyama, Hiroshi and Endo, Masayuki and Takahashi, Yuki and Takakura, Yoshinobu and Nishikawa, Makiya

Journal:

Nanoscale (2019): 23416-23422

Combined effect of propranolol, vincristine and bevacizumab on HUVECs and BJ cells.

Authors:

Bota, Mădălina and Fischer-Fodor, Eva and Bochiș, Ovidiu-Vasile and Cenariu, Mihai and Popa, Gheorghe and Blag, Cristina Lucia and Tătaru, Alexandru

Journal:

Experimental and therapeutic medicine (2019): 307-315

Cell-type-specific quantification of protein synthesis in vivo.

Authors:

Hidalgo San Jose, Lorena and Signer, Robert A J

Journal:

Nature protocols (2019): 441-460

Physical properties

Molecular weight	831.88
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.98
Correction factor (280 nm)	0.46
Extinction coefficient (cm ^-1 M ^-1)	80000 ¹
Excitation (nm)	468
Emission (nm)	493
Quantum yield	~0.8 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on October 23, 2026