Name: iFluor® 532 maleimide
Brand: AAT Bioquest
SKU: 1061
Price: 237 USD
Availability: InStock

iFluor® 532 maleimide

iFluor® 532 Maleimide is a thiol-reactive fluorescent dye for selective covalent labeling of antibodies, proteins, and other thiol-containing biomolecules in fluorescence-based research applications.

Thiol-Reactive Functionality: Maleimide group selectively reacts with free thiols, including cysteine residues.
Spectral Properties: Excitation at 537 nm and emission at 560 nm.
Fluorescence Performance: Bright, photostable fluorescence with a quantum yield of 0.68.
Spectral Compatibility: Alternative to Alexa Fluor® 532.
Applications: Suitable for fluorescent conjugation of antibodies and other thiol-containing proteins.

<p>HeLa cells were stained with mouse anti-tubulin followed with iFluor<sup>TM</sup> 532 goat anti-mouse IgG (H+L). Cell nuclei were stained with DAPI (Cat#17507).</p>

Protocol

SDS

Catalog	Size	Price	Quantity
1061	1 mg	Price

Overview

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, and 633 nm). iFluor® 532 dyes have fluorescence excitation and emission maxima of ~537 nm and ~560 nm respectively. These spectral characteristics make them an excellent alternative to Alexa Fluor® 532 labeling dye (Alexa Fluor® is the trademark of Invitrogen). iFluor® 350 maleimide is reasonably stable and shows good reactivity and selectivity with the thiol group.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 532 maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	117.543 µL	587.717 µL	1.175 mL	5.877 mL	11.754 mL
5 mM	23.509 µL	117.543 µL	235.087 µL	1.175 mL	2.351 mL
10 mM	11.754 µL	58.772 µL	117.543 µL	587.717 µL	1.175 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	850.75 g/mol	=	mL	x	mM	=	mmol

Citations

View all 3 citations: Citation Explorer

Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification

Authors:

Gao, D and Liu, N and Li, Y and Zhang, Y and Liu, G and others, undefined

Journal:

Metabolomics (Los Angel) (2017): 2153--0769

Suramin inhibits cullin-RING E3 ubiquitin ligases

Authors:

Wu, Kenneth and Chong, Robert A and Yu, Qing and Bai, Jin and Spratt, Donald E and Ching, Kevin and Lee, Chan and Miao, Haibin and Tappin, Inger and Hurwitz, Jerard and others, undefined

Journal:

Proceedings of the National Academy of Sciences (2016): E2011--E2018

Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function

Authors:

Piccard, Helene and Berghmans, Nele and Korpos, Eva and Dillen, Chris and Aelst, Ilse Van and Li, S and ra , undefined and Martens, Erik and Liekens, S and ra , undefined and Noppen, Sam and Damme, Jo Van and others, undefined

Journal:

International Journal of Cancer (2012): E425--E436

References

View all 49 references: Citation Explorer

Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes

Authors:

Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.

Journal:

Mol Biosyst (2011): 1766

Visualizing dengue virus through Alexa Fluor labeling

Authors:

Zhang S, Tan HC, Ooi EE.

Journal:

J Vis Exp. (2011)

Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells

Authors:

Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.

Journal:

Biochem Biophys Res Commun (2011): 211

Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594

Authors:

Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.

Journal:

Zhen Ci Yan Jiu (2011): 262

Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers

Authors:

Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.

Journal:

J Microbiol Methods (2011): 119

Physical properties

Molecular weight	850.75
Solvent	DMSO

Spectral properties

Absorbance (nm)	536
Correction factor (260 nm)	0.26
Correction factor (280 nm)	0.16
Extinction coefficient (cm ^-1 M ^-1)	90000 ¹
Excitation (nm)	537
Emission (nm)	560
Quantum yield	0.68 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on October 28, 2026