iFluor® 540 maleimide
Ordering information
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Catalog Number | |
Unit Size | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 777.82 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
iFluor® 540 succinimidyl ester |
Overview | ![]() ![]() |
See also: iFluor® Dyes and Kits
Molecular weight 777.82 |
iFluor® 540 maleimide selectively reacts with thiol group of a biomolecule. It is widely used to label the reduced antibodies and other thiol-containing biomolecules such as peptides and thiol-modified oligos. iFluor®540 is a fluorophore from the iFluor family, which is known for its bright red fluorescence and compatibility with various fluorescence techniques and instruments. When excited with light in the green to yellow range (around 530 to 545 nm), iFluor 540 emits red fluorescence with an emission peak typically around 560 to 570 nanometers. iFluor 540 can be conjugated to a variety of biomolecules, including antibodies, proteins, nucleic acids, and small molecules, enabling their visualization and tracking in cells and tissues. It is commonly used in fluorescence microscopy, immunohistochemistry, flow cytometry, and other fluorescence-based assays.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 540 maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 128.564 µL | 642.822 µL | 1.286 mL | 6.428 mL | 12.856 mL |
5 mM | 25.713 µL | 128.564 µL | 257.129 µL | 1.286 mL | 2.571 mL |
10 mM | 12.856 µL | 64.282 µL | 128.564 µL | 642.822 µL | 1.286 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
iFluor® 350 maleimide | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
iFluor® 488 maleimide | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 555 maleimide | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
iFluor® 647 maleimide | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 |
iFluor® 680 maleimide | 684 | 701 | 2200001 | 0.231 | 0.097 | 0.094 |
iFluor® 700 maleimide | 690 | 713 | 2200001 | 0.231 | 0.09 | 0.04 |
iFluor® 750 maleimide | 757 | 779 | 2750001 | 0.121 | 0.044 | 0.039 |
iFluor® 790 maleimide | 787 | 812 | 2500001 | 0.131 | 0.1 | 0.09 |
iFluor® 800 maleimide | 801 | 820 | 2500001 | 0.111 | 0.03 | 0.08 |
Show More (20) |
References
View all 8 references: Citation Explorer
A molecular rack and pinion actuates a cell-surface adhesin and enables bacterial gliding motility.
Authors: Shrivastava, Abhishek and Berg, Howard C
Journal: Science advances (2020): eaay6616
Authors: Shrivastava, Abhishek and Berg, Howard C
Journal: Science advances (2020): eaay6616
Protein Environment and DNA Orientation Affect Protein-Induced Cy3 Fluorescence Enhancement.
Authors: Nguyen, Binh and Ciuba, Monika A and Kozlov, Alexander G and Levitus, Marcia and Lohman, Timothy M
Journal: Biophysical journal (2019): 66-73
Authors: Nguyen, Binh and Ciuba, Monika A and Kozlov, Alexander G and Levitus, Marcia and Lohman, Timothy M
Journal: Biophysical journal (2019): 66-73
Toward a solid-phase nucleic acid hybridization assay within microfluidic channels using immobilized quantum dots as donors in fluorescence resonance energy transfer.
Authors: Chen, Lu and Algar, W Russ and Tavares, Anthony J and Krull, Ulrich J
Journal: Analytical and bioanalytical chemistry (2011): 133-41
Authors: Chen, Lu and Algar, W Russ and Tavares, Anthony J and Krull, Ulrich J
Journal: Analytical and bioanalytical chemistry (2011): 133-41
Electrical readout of protein microarrays on regular glass slides.
Authors: Bonilla, Diana and Mallén, Maria and de la Rica, Roberto and Fernández-Sánchez, César and Baldi, Antonio
Journal: Analytical chemistry (2011): 1726-31
Authors: Bonilla, Diana and Mallén, Maria and de la Rica, Roberto and Fernández-Sánchez, César and Baldi, Antonio
Journal: Analytical chemistry (2011): 1726-31
Thiazole orange and Cy3: improvement of fluorescent DNA probes with use of short range electron transfer.
Authors: Menacher, Florian and Rubner, Moritz and Berndl, Sina and Wagenknecht, Hans-Achim
Journal: The Journal of organic chemistry (2008): 4263-6
Authors: Menacher, Florian and Rubner, Moritz and Berndl, Sina and Wagenknecht, Hans-Achim
Journal: The Journal of organic chemistry (2008): 4263-6
Probing orientations of single fluorescent labels on a peptide reversibly binding to the human delta-opioid receptor.
Authors: Tokimoto, Takahira and Bethea, Tomika R C and Zhou, Min and Ghosh, Indraneel and Wirth, Mary J
Journal: Applied spectroscopy (2007): 130-7
Authors: Tokimoto, Takahira and Bethea, Tomika R C and Zhou, Min and Ghosh, Indraneel and Wirth, Mary J
Journal: Applied spectroscopy (2007): 130-7
Laser scanning and electron microscopic evidence for rapid and specific in vivo labelling of cholinergic neurons in the rat basal forebrain with fluorochromated antibodies.
Authors: Kacza, J and Grosche, J and Seeger, J and Brauer, K and Brückner, G and Härtig, W
Journal: Brain research (2000): 232-8
Authors: Kacza, J and Grosche, J and Seeger, J and Brauer, K and Brückner, G and Härtig, W
Journal: Brain research (2000): 232-8
Stimulated release of fluorescently labeled IgE fragments that efficiently accumulate in secretory granules after endocytosis in RBL-2H3 mast cells.
Authors: Xu, K and Williams, R M and Holowka, D and Baird, B
Journal: Journal of cell science (1998): 2385-96
Authors: Xu, K and Williams, R M and Holowka, D and Baird, B
Journal: Journal of cell science (1998): 2385-96
Application notes
A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
FAQ
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