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iFluor® 450 maleimide
AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, and 633 nm). Under the same conditions, iFluor® 450 dye conjugates are significantly brighter than the corresponding bioconjugates of other spectrally similar dyes. iFluor® 450 maleimide is stable and shows good reactivity and selectivity with the thiol group.
HL-60 cells were incubated with (Red, +) or without (Green, -) Anti-human HLA-ABC (W6/32 mAb), followed by iFluor® 450 goat anti-mouse IgG conjugate. The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in FITC channel.
HL-60 cells were incubated with (Red, +) or without (Green, -) Anti-human HLA-ABC (W6/32 mAb), followed by iFluor® 450 goat anti-mouse IgG conjugate. The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in FITC channel.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
10571 mg
Price
 
Physical properties
Molecular weight527.55
SolventDMSO
Spectral properties
Correction factor (260 nm)0.45
Correction factor (280 nm)0.27
Extinction coefficient (cm -1 M -1)
40000
1
Excitation (nm)451
Emission (nm)502
Quantum yield
0.82
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Page updated on October 12, 2025