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iFluor® 555 maleimide
AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, and 633 nm). iFluor® 555 dyes have fluorescence excitation and emission maxima of ~557 nm and ~570 nm respectively. The iFluor® 555 family has spectral properties essentially identical to those of Cy3® (Cy3® is the trademark of GE Healthcare). Compared to Cy3 probes, the iFluor® 555 family has much stronger fluorescence and higher photostability. Their fluorescence is pH-independent from pH 3 to 11. These spectral characteristics make this new dye family a superior alternative to Cy3®. iFluor® 555 family has become an excellent replacement for Cy3® and Alexa Fluor® 555 labeling dye (Cy3® and Alexa Fluor® are the trademarks of Invitrogen and GE Health Care). iFluor® 555 maleimide is reasonably stable and shows good reactivity and selectivity with the thiol group.
<p>HeLa cells were stained with mouse anti-tubulin followed with iFluor<sup>TM</sup>&nbsp;555 goat anti-mouse IgG (H+L) (red); actin filaments were stained with Phalloidin-iFluor<sup>TM</sup> 488 conjugate (green); and nuclei were stained with DAPI&nbsp;(blue).</p>
<p>HeLa cells were stained with mouse anti-tubulin followed with iFluor<sup>TM</sup>&nbsp;555 goat anti-mouse IgG (H+L) (red); actin filaments were stained with Phalloidin-iFluor<sup>TM</sup> 488 conjugate (green); and nuclei were stained with DAPI&nbsp;(blue).</p>
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
10631 mg
Price
 
Physical properties
Molecular weight947.93
SolventDMSO
Spectral properties
Correction factor (260 nm)0.23
Correction factor (280 nm)0.14
Extinction coefficient (cm -1 M -1)
100000
1
Excitation (nm)557
Emission (nm)570
Quantum yield
0.64
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Page updated on October 8, 2025