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AAT Bioquest

iFluor® 670 acid

AAT Bioquest's iFluor® dyes are designed for precise protein labeling, particularly for antibodies. These dyes exhibit excellent brightness, high photostability, and minimal quenching when conjugated to proteins. They can be well excited by the major laser lines used in fluorescence instrumentation, including 350, 405, 488, 555, and 633 nm. iFluor® 670, specifically, is a red-laser excitable dye optimized for flow cytometry applications, including spectral flow cytometry. It has spectral characteristics similar to Cy5B but demonstrates a higher fluorescence quantum yield and superior photostability. Additionally, iFluor® 670 maintains consistent fluorescence across a broad pH range (pH 3-11), making it a robust alternative to Cy5B.

iFluor® 670 acid is a non-reactive compound that can be employed as a reference standard in studies utilizing iFluor® 670 conjugates. It is also suitable for use as a control in confocal microscopy, immunocytochemistry (ICC), high-content screening (HCS), flow cytometry, and live cell imaging applications. Furthermore, it can be utilized in the synthesis of activated esters and STP and can be coupled to hydrazines, hydroxylamines, or amines in aqueous solutions using water-soluble carbodiimides (e.g., EDAC). This allows for the conjugation of the dye to amino-containing molecules, such as proteins, antibodies, amine-modified oligonucleotides, and peptides.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
iFluor® 790 acid78781225000010.1310.10.09
iFluor® 800 acid80182025000010.1110.030.08
iFluor® 810 acid81182225000010.0510.090.15
iFluor® 820 acid82285025000010.110.16
iFluor® 860 acid85387825000010.10.14
iFluor® 840 acid8368792000001-0.20.09
iFluor® 830 acid830867----
iFluor® 670 Styramide *Replacement for Opal 690*67168220000010.5510.030.033
iFluor® 350 acid3454502000010.9510.830.23
iFluor® 405 acid4034273700010.9110.480.77
iFluor® 430 acid4334984000010.7810.680.3
iFluor® 450 acid4515024000010.8210.450.27
iFluor® 647 acid65667025000010.2510.030.03
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Page updated on December 6, 2024

Ordering information

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Catalog Number2911
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Additional ordering information

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Physical properties

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.03

Correction Factor (280 nm)

0.033

Extinction coefficient (cm -1 M -1)

2000001

Excitation (nm)

671

Emission (nm)

682

Quantum yield

0.551

Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
With EDAC or other equivalent activating coupling agents, fluorescent dyes, such as iFluor® 670, can react readily with the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.
With EDAC or other equivalent activating coupling agents, fluorescent dyes, such as iFluor® 670, can react readily with the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.
With EDAC or other equivalent activating coupling agents, fluorescent dyes, such as iFluor® 670, can react readily with the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.