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iFluor® 840 acid

In vivo fluorescence imaging uses a sensitive camera to detect the fluorescence emission from fluorophores in whole-body living small animals. To overcome the photon attenuation in living tissue, fluorophores with long emission at the infrared (IR) region are generally preferred. Recent advances in imaging strategies and reporter techniques for in vivo fluorescence imaging include novel approaches to improve the specificity and affinity of the probes and to modulate and amplify the signal at target sites for enhanced sensitivity. Further emerging developments aim to achieve high-resolution, multimodality, and lifetime-based in vivo fluorescence imaging. Our iFluor® 830 is designed to label proteins and other biomolecules with infrared fluorescence. Conjugates prepared with iFluor® 830 have excitation and emission in the IR range. iFluor® 840 dye emission is well separated from commonly used far-red fluorophores such as Cy5, Cy7, or allophycocyanin (APC), facilitating multicolor analysis. This fluorophore is also useful for small animal in vivo imaging applications or other imaging applications requiring IR detection.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 840 acid to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM70.126 µL350.629 µL701.257 µL3.506 mL7.013 mL
5 mM14.025 µL70.126 µL140.251 µL701.257 µL1.403 mL
10 mM7.013 µL35.063 µL70.126 µL350.629 µL701.257 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
iFluor® 790 acid78781225000010.1310.10.09
iFluor® 800 acid80182025000010.1110.030.08
iFluor® 810 acid81182225000010.0510.090.15
iFluor® 820 acid82285025000010.110.16
iFluor® 860 acid85387825000010.10.14
iFluor® 830 acid830867----
iFluor® 670 acid67168220000010.5510.030.033
iFluor® 350 acid3454502000010.9510.830.23
iFluor® 405 acid4034273700010.9110.480.77
iFluor® 430 acid4334984000010.7810.680.3
iFluor® 450 acid4515024000010.8210.450.27
iFluor® 647 acid65667025000010.2510.030.03
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Citations

View all 8 citations: Citation Explorer
Block Face Scanning Electron Microscopy of Fluorescently Labeled Axons Without Using Near Infra-Red Branding
Authors: Maclachlan, C., Sahlender, D. A., Hayashi, S., Molnar, Z., Knott, G.
Journal: Front Neuroanat (2018): 88
Bioactive magnetic near Infra-Red fluorescent core-shell iron oxide/human serum albumin nanoparticles for controlled release of growth factors for augmentation of human mesenchymal stem cell growth and differentiation
Authors: Levy, I., Sher, I., Corem-Salkmon, E., Ziv-Polat, O., Meir, A., Treves, A. J., Nagler, A., Kalter-Leibovici, O., Margel, S., Rotenstreich, Y.
Journal: J Nanobiotechnology (2015): 34
Novel near infra-red fluorescent pH sensors based on 1-aminoperylene bisimides covalently grafted onto poly(acryloylmorpholine)
Authors: Aigner, D., Borisov, S. M., Petritsch, P., Klimant, I.
Journal: Chem Commun (Camb) (2013): 2139-41
In vitro and ex vivo evaluation of smart infra-red fluorescent caspase-3 probes for molecular imaging of cardiovascular apoptosis
Authors: Debunne, M., Portal, C., Delest, B., Brakenhielm, E., Lallem and , F., Henry, J. P., Ligeret, H., Noack, P., Massonneau, M., Romieu, A., Renard, P. Y., Thuillez, C., Richard, V.
Journal: Int J Mol Imaging (2011): 413290
Simple sensitive and simultaneous high-performance liquid chromatography method of glucoconjugated and non-glucoconjugated porphyrins and chlorins using near infra-red fluorescence detection
Authors: Canada-Canada, F., Bautista-Sanchez, A., Taverna, M., Prognon, P., Maillard, P., Grierson, D. S., Kasselouri, A.
Journal: J Chromatogr B Analyt Technol Biomed Life Sci (2005): 166-72

References

View all 19 references: Citation Explorer
Nanovesicle delivery to the liver via retinol binding protein and platelet-derived growth factor receptors: how targeting ligands affect biodistribution
Authors: Hsu, Ching-Yun and Chen, Chun-Han and Aljuffali, Ibrahim A and Dai, You-Shan and Fang, Jia-You
Journal: Nanomedicine (2017)
A protocol for imaging alternative splicing regulation in vivo using fluorescence reporters in transgenic mice
Authors: Bonano VI, Oltean S, Garcia-Blanco MA.
Journal: Nat Protoc (2007): 2166
A target cell-specific activatable fluorescence probe for in vivo molecular imaging of cancer based on a self-quenched avidin-rhodamine conjugate
Authors: Hama Y, Urano Y, Koyama Y, Kamiya M, Bernardo M, Paik RS, Shin IS, Paik CH, Choyke PL, Kobayashi H.
Journal: Cancer Res (2007): 2791
Fluorescence imaging in vivo: recent advances
Authors: Rao J, Dragulescu-Andrasi A, Yao H.
Journal: Curr Opin Biotechnol (2007): 17
Ex vivo fluorescence imaging of normal and malignant urothelial cells to enhance early diagnosis
Authors: Steenkeste K, Lecart S, Deniset A, Pernot P, Eschwege P, Ferlicot S, Leveque-Fort S, Bri and et R, Fontaine-Aupart MP.
Journal: Photochem Photobiol (2007): 1157
Page updated on December 11, 2024

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Catalog Number1401
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Physical properties

Molecular weight

1426.01

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.2

Correction Factor (280 nm)

0.09

Extinction coefficient (cm -1 M -1)

2000001

Excitation (nm)

836

Emission (nm)

879

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
With EDAC or other equivalent activating coupling agents, iFluor 840 acid can react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.
With EDAC or other equivalent activating coupling agents, iFluor 840 acid can react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.
With EDAC or other equivalent activating coupling agents, iFluor 840 acid can react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.