iFluor® 840 acid
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1426.01 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.2 |
Correction Factor (280 nm) | 0.09 |
Extinction coefficient (cm -1 M -1) | 2000001 |
Excitation (nm) | 836 |
Emission (nm) | 879 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Alternative formats
iFluor® 840 succinimidyl ester |
iFluor® 840 maleimide |
Overview | ![]() ![]() |
See also: iFluor® Dyes and Kits
Molecular weight 1426.01 | Correction Factor (260 nm) 0.2 | Correction Factor (280 nm) 0.09 | Extinction coefficient (cm -1 M -1) 2000001 | Excitation (nm) 836 | Emission (nm) 879 |
In vivo fluorescence imaging uses a sensitive camera to detect the fluorescence emission from fluorophores in whole-body living small animals. To overcome the photon attenuation in living tissue, fluorophores with long emission at the infrared (IR) region are generally preferred. Recent advances in imaging strategies and reporter techniques for in vivo fluorescence imaging include novel approaches to improve the specificity and affinity of the probes and to modulate and amplify the signal at target sites for enhanced sensitivity. Further emerging developments aim to achieve high-resolution, multimodality, and lifetime-based in vivo fluorescence imaging. Our iFluor® 830 is designed to label proteins and other biomolecules with infrared fluorescence. Conjugates prepared with iFluor® 830 have excitation and emission in the IR range. iFluor® 840 dye emission is well separated from commonly used far-red fluorophores such as Cy5, Cy7, or allophycocyanin (APC), facilitating multicolor analysis. This fluorophore is also useful for small animal in vivo imaging applications or other imaging applications requiring IR detection.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 840 acid to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 70.126 µL | 350.629 µL | 701.257 µL | 3.506 mL | 7.013 mL |
5 mM | 14.025 µL | 70.126 µL | 140.251 µL | 701.257 µL | 1.403 mL |
10 mM | 7.013 µL | 35.063 µL | 70.126 µL | 350.629 µL | 701.257 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
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Spectral properties
Correction Factor (260 nm) | 0.2 |
Correction Factor (280 nm) | 0.09 |
Extinction coefficient (cm -1 M -1) | 2000001 |
Excitation (nm) | 836 |
Emission (nm) | 879 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
iFluor® 790 acid | 787 | 812 | 2500001 | 0.131 | 0.1 | 0.09 |
iFluor® 800 acid | 801 | 820 | 2500001 | 0.111 | 0.03 | 0.08 |
iFluor® 810 acid | 811 | 822 | 2500001 | 0.051 | 0.09 | 0.15 |
iFluor® 820 acid | 822 | 850 | 2500001 | 0.11 | 0.16 | |
iFluor® 860 acid | 853 | 878 | 2500001 | 0.1 | 0.14 | |
iFluor® 830 acid | 830 | 867 | - | - | - | - |
Images
Citations
View all 8 citations: Citation Explorer
Block Face Scanning Electron Microscopy of Fluorescently Labeled Axons Without Using Near Infra-Red Branding
Authors: Maclachlan, C., Sahlender, D. A., Hayashi, S., Molnar, Z., Knott, G.
Journal: Front Neuroanat (2018): 88
Authors: Maclachlan, C., Sahlender, D. A., Hayashi, S., Molnar, Z., Knott, G.
Journal: Front Neuroanat (2018): 88
Bioactive magnetic near Infra-Red fluorescent core-shell iron oxide/human serum albumin nanoparticles for controlled release of growth factors for augmentation of human mesenchymal stem cell growth and differentiation
Authors: Levy, I., Sher, I., Corem-Salkmon, E., Ziv-Polat, O., Meir, A., Treves, A. J., Nagler, A., Kalter-Leibovici, O., Margel, S., Rotenstreich, Y.
Journal: J Nanobiotechnology (2015): 34
Authors: Levy, I., Sher, I., Corem-Salkmon, E., Ziv-Polat, O., Meir, A., Treves, A. J., Nagler, A., Kalter-Leibovici, O., Margel, S., Rotenstreich, Y.
Journal: J Nanobiotechnology (2015): 34
Novel near infra-red fluorescent pH sensors based on 1-aminoperylene bisimides covalently grafted onto poly(acryloylmorpholine)
Authors: Aigner, D., Borisov, S. M., Petritsch, P., Klimant, I.
Journal: Chem Commun (Camb) (2013): 2139-41
Authors: Aigner, D., Borisov, S. M., Petritsch, P., Klimant, I.
Journal: Chem Commun (Camb) (2013): 2139-41
In vitro and ex vivo evaluation of smart infra-red fluorescent caspase-3 probes for molecular imaging of cardiovascular apoptosis
Authors: Debunne, M., Portal, C., Delest, B., Brakenhielm, E., Lallem and , F., Henry, J. P., Ligeret, H., Noack, P., Massonneau, M., Romieu, A., Renard, P. Y., Thuillez, C., Richard, V.
Journal: Int J Mol Imaging (2011): 413290
Authors: Debunne, M., Portal, C., Delest, B., Brakenhielm, E., Lallem and , F., Henry, J. P., Ligeret, H., Noack, P., Massonneau, M., Romieu, A., Renard, P. Y., Thuillez, C., Richard, V.
Journal: Int J Mol Imaging (2011): 413290
Simple sensitive and simultaneous high-performance liquid chromatography method of glucoconjugated and non-glucoconjugated porphyrins and chlorins using near infra-red fluorescence detection
Authors: Canada-Canada, F., Bautista-Sanchez, A., Taverna, M., Prognon, P., Maillard, P., Grierson, D. S., Kasselouri, A.
Journal: J Chromatogr B Analyt Technol Biomed Life Sci (2005): 166-72
Authors: Canada-Canada, F., Bautista-Sanchez, A., Taverna, M., Prognon, P., Maillard, P., Grierson, D. S., Kasselouri, A.
Journal: J Chromatogr B Analyt Technol Biomed Life Sci (2005): 166-72
An infra-red light-transmitting aperture controller for use in single-cell fluorescence photometry
Authors: Mahaut-Smith, M. P.
Journal: J Microsc (1998): 60-6
Authors: Mahaut-Smith, M. P.
Journal: J Microsc (1998): 60-6
Photosynthetic induction in C4 leaves : An investigation using infra-red gas analysis and chlorophyll a fluorescence
Authors: Furbank, R. T., Walker, D. A.
Journal: Planta (1985): 75-83
Authors: Furbank, R. T., Walker, D. A.
Journal: Planta (1985): 75-83
Fluorescent infra-red angiography of the fundus oculi using indocyanine green dye
Authors: Chopdar, A., Turk, A. M., Hill, D. W.
Journal: Trans Ophthalmol Soc U K (1978): 142-6
Authors: Chopdar, A., Turk, A. M., Hill, D. W.
Journal: Trans Ophthalmol Soc U K (1978): 142-6
References
View all 19 references: Citation Explorer
Nanovesicle delivery to the liver via retinol binding protein and platelet-derived growth factor receptors: how targeting ligands affect biodistribution
Authors: Hsu, Ching-Yun and Chen, Chun-Han and Aljuffali, Ibrahim A and Dai, You-Shan and Fang, Jia-You
Journal: Nanomedicine (2017)
Authors: Hsu, Ching-Yun and Chen, Chun-Han and Aljuffali, Ibrahim A and Dai, You-Shan and Fang, Jia-You
Journal: Nanomedicine (2017)
A target cell-specific activatable fluorescence probe for in vivo molecular imaging of cancer based on a self-quenched avidin-rhodamine conjugate
Authors: Hama Y, Urano Y, Koyama Y, Kamiya M, Bernardo M, Paik RS, Shin IS, Paik CH, Choyke PL, Kobayashi H.
Journal: Cancer Res (2007): 2791
Authors: Hama Y, Urano Y, Koyama Y, Kamiya M, Bernardo M, Paik RS, Shin IS, Paik CH, Choyke PL, Kobayashi H.
Journal: Cancer Res (2007): 2791
Fluorescence imaging in vivo: recent advances
Authors: Rao J, Dragulescu-Andrasi A, Yao H.
Journal: Curr Opin Biotechnol (2007): 17
Authors: Rao J, Dragulescu-Andrasi A, Yao H.
Journal: Curr Opin Biotechnol (2007): 17
Ex vivo fluorescence imaging of normal and malignant urothelial cells to enhance early diagnosis
Authors: Steenkeste K, Lecart S, Deniset A, Pernot P, Eschwege P, Ferlicot S, Leveque-Fort S, Bri and et R, Fontaine-Aupart MP.
Journal: Photochem Photobiol (2007): 1157
Authors: Steenkeste K, Lecart S, Deniset A, Pernot P, Eschwege P, Ferlicot S, Leveque-Fort S, Bri and et R, Fontaine-Aupart MP.
Journal: Photochem Photobiol (2007): 1157
In vivo monitoring the fate of Cy5.5-Tat labeled T lymphocytes by quantitative near-infrared fluorescence imaging during acute brain inflammation in a rat model of experimental autoimmune encephalomyelitis
Authors: Berger C, Gremlich HU, Schmidt P, Cannet C, Kneuer R, Hiest and P, Rausch M, Rudin M.
Journal: J Immunol Methods (2007): 65
Authors: Berger C, Gremlich HU, Schmidt P, Cannet C, Kneuer R, Hiest and P, Rausch M, Rudin M.
Journal: J Immunol Methods (2007): 65
A protocol for imaging alternative splicing regulation in vivo using fluorescence reporters in transgenic mice
Authors: Bonano VI, Oltean S, Garcia-Blanco MA.
Journal: Nat Protoc (2007): 2166
Authors: Bonano VI, Oltean S, Garcia-Blanco MA.
Journal: Nat Protoc (2007): 2166
In vivo imaging of the bronchial wall microstructure using fibered confocal fluorescence microscopy
Authors: Thiberville L, Moreno-Swirc S, Vercauteren T, Peltier E, Cave C, Bourg Heckly G.
Journal: Am J Respir Crit Care Med (2007): 22
Authors: Thiberville L, Moreno-Swirc S, Vercauteren T, Peltier E, Cave C, Bourg Heckly G.
Journal: Am J Respir Crit Care Med (2007): 22
In Vivo Fluorescence Microscopic Imaging for Dynamic Quantitative Assessment of Intestinal Mucosa Permeability in Mice
Authors: Szabo A, Vollmar B, Boros M, Menger MD.
Journal: J Surg Res. (2007)
Authors: Szabo A, Vollmar B, Boros M, Menger MD.
Journal: J Surg Res. (2007)
In vivo spectral fluorescence imaging of submillimeter peritoneal cancer implants using a lectin-targeted optical agent
Authors: Hama Y, Urano Y, Koyama Y, Kamiya M, Bernardo M, Paik RS, Krishna MC, Choyke PL, Kobayashi H.
Journal: Neoplasia (2006): 607
Authors: Hama Y, Urano Y, Koyama Y, Kamiya M, Bernardo M, Paik RS, Krishna MC, Choyke PL, Kobayashi H.
Journal: Neoplasia (2006): 607
In vivo imaging of green fluorescent protein-expressing cells in transgenic animals using fibred confocal fluorescence microscopy
Authors: Al-Gubory KH, Houdebine LM.
Journal: Eur J Cell Biol (2006): 837
Authors: Al-Gubory KH, Houdebine LM.
Journal: Eur J Cell Biol (2006): 837
Application notes
iFluor® Dye Selection Guide
A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
FAQ
Are Cell Navigator® Cell Plasma Membrane Staining Kits suitable for cell culture medium samples?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?
Are there any calcium indicators that don't require probenecid (PBC)?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?
Are there any calcium indicators that don't require probenecid (PBC)?