iFluor® 430 acid

iFluor® 430 is a blue fluorescent dye similar to Alexa Fluor® 430, but it offers better absorption, brightness, and pH stability.

• High performance: Brighter fluorescence and stronger absorption than Alexa Fluor® 430, with excellent photostability.
• Direct equivalent: Serves as a superior replacement for Alexa Fluor® 430 acid with enhanced solubility and spectral properties.
• Conjugation-ready chemistry: Can be converted into reactive esters for labeling proteins, peptides, and oligonucleotides.
• Broad applications: Ideal for ICC, flow cytometry, live-cell imaging, and multicolor fluorescence microscopy.

iFluor® 430 is a blue-fluorescent dye that is excited by the 405 nm laser line and emits green fluorescence with a peak at 498 nm. It offers brighter signal and stronger absorption than Alexa Fluor® 430, with improved aqueous solubility and excellent stability across a wide pH range (pH 3 to11). These properties make iFluor® 430 ideal for demanding multicolor applications like flow cytometry and confocal microscopy, where spectral separation and photostability are critical.

iFluor® 430 acid is the non-reactive version of the dye and serves as a reference standard or fluorescence control in assays using iFluor® 430 conjugates. It is suitable for use in ICC, confocal microscopy, flow cytometry, HCS, and live-cell imaging. It can also be chemically modified to generate activated esters or STP derivatives and coupled to hydrazines, hydroxylamines, or amines using water-soluble carbodiimides such as EDAC, enabling efficient conjugation to proteins, antibodies, peptides, and amine-modified oligonucleotides.