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iFluor® 790 acid

With EDAC or other equivalent activating coupling agents, fluorescent dyes can react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.
With EDAC or other equivalent activating coupling agents, fluorescent dyes can react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.
Ordering information
Price ()
Catalog Number1360
Unit Size
Find Distributor
Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight1279.29
SolventDMSO
Spectral properties
Correction Factor (260 nm)0.1
Correction Factor (280 nm)0.09
Extinction coefficient (cm -1 M -1)2500001
Excitation (nm)787
Emission (nm)812
Quantum yield0.131
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


Molecular weight
1279.29
Correction Factor (260 nm)
0.1
Correction Factor (280 nm)
0.09
Extinction coefficient (cm -1 M -1)
2500001
Excitation (nm)
787
Emission (nm)
812
Quantum yield
0.131
In vivo fluorescence imaging uses a sensitive camera to detect fluorescence emission from fluorophores in whole-body living small animals. To overcome the photon attenuation in living tissue, fluorophores with long emission at the near-infrared (NIR) region are generally preferred, including widely used small indocarbocyanine dyes. Recent advances in imaging strategies and reporter techniques for in vivo fluorescence imaging include novel approaches to improve the specificity and affinity of the probes and to modulate and amplify the signal at target sites for enhanced sensitivity. Further emerging developments are aiming to achieve high-resolution, multimodality and lifetime-based in vivo fluorescence imaging. Our iFluor® 790 is designed to label proteins and other biomolecules with near infrared fluorescence. Conjugates prepared with iFluor® 790 have the excitation and emission spectra similar to that of indocyanine green (ICG) and the IRDye® 800 dye, with 783/814 nm excitation/emission maxima. iFluor® 790 dye emission is well separated from commonly used far-red fluorophores such as Cy5, Cy7 or allophycocyanin (APC), facilitating multicolor analysis. This fluorophore is also useful for small animal in-vivo imaging applications or for other imaging applications that require NIR detections such as the two-color western applications with the LI-COR® Odyssey® infrared imaging system.

Platform


Fluorescence microplate reader

Excitation782 nm
Emission811 nm
Cutoff790 nm
Recommended plateSolid black

Example protocol


AT A GLANCE

Important notes
It is important to store at <-15 °C and should be stored in cool, dark place.

It can be used within 24 months from the date of receipt.

PREPARATION OF WORKING SOLUTION

iFluor™ 790 acid working solution:
Add DMF, DMSO or water to make iFluor™ 790 acid working solution of desired concentration.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 790 acid to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM78.168 µL390.842 µL781.684 µL3.908 mL7.817 mL
5 mM15.634 µL78.168 µL156.337 µL781.684 µL1.563 mL
10 mM7.817 µL39.084 µL78.168 µL390.842 µL781.684 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
/=x=

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Correction Factor (260 nm)0.1
Correction Factor (280 nm)0.09
Extinction coefficient (cm -1 M -1)2500001
Excitation (nm)787
Emission (nm)812
Quantum yield0.131

Citations


View all 5 citations: Citation Explorer
Lipid nanoparticulate drug delivery system for the treatment of hepatic fibrosis
Authors: Mukherjee, Swarupananda and Dutta, Ayon and Ash, Dipanjana
Journal: Archives of Hepatitis Research (2021): 001--003
Lipid-based nanoparticle technologies for liver targeting
Authors: B{\"o}ttger, Roland and Pauli, Griffin and Chao, Po-Han and Fayez, Nojoud AL and Hohenwarter, Lukas and Li, Shyh-Dar
Journal: Advanced drug delivery reviews (2020): 79--101
Self-assembly and directed assembly of lipid nanocarriers for prevention of liver fibrosis in obese rats: a comparison with the therapy of bariatric surgery
Authors: Chen, Chun-Han and Chen, Chih-Jung and Elzoghby, Ahmed O and Yeh, Ta-Sen and Fang, Jia-You
Journal: Nanomedicine (2018)
Nanovesicle delivery to the liver via retinol binding protein and platelet-derived growth factor receptors: how targeting ligands affect biodistribution
Authors: Hsu, Ching-Yun and Chen, Chun-Han and Aljuffali, Ibrahim A and Dai, You-Shan and Fang, Jia-You
Journal: Nanomedicine (2017)

References


View all 18 references: Citation Explorer
A target cell-specific activatable fluorescence probe for in vivo molecular imaging of cancer based on a self-quenched avidin-rhodamine conjugate
Authors: Hama Y, Urano Y, Koyama Y, Kamiya M, Bernardo M, Paik RS, Shin IS, Paik CH, Choyke PL, Kobayashi H.
Journal: Cancer Res (2007): 2791
Fluorescence imaging in vivo: recent advances
Authors: Rao J, Dragulescu-Andrasi A, Yao H.
Journal: Curr Opin Biotechnol (2007): 17
Ex vivo fluorescence imaging of normal and malignant urothelial cells to enhance early diagnosis
Authors: Steenkeste K, Lecart S, Deniset A, Pernot P, Eschwege P, Ferlicot S, Leveque-Fort S, Bri and et R, Fontaine-Aupart MP.
Journal: Photochem Photobiol (2007): 1157
In vivo monitoring the fate of Cy5.5-Tat labeled T lymphocytes by quantitative near-infrared fluorescence imaging during acute brain inflammation in a rat model of experimental autoimmune encephalomyelitis
Authors: Berger C, Gremlich HU, Schmidt P, Cannet C, Kneuer R, Hiest and P, Rausch M, Rudin M.
Journal: J Immunol Methods (2007): 65
A protocol for imaging alternative splicing regulation in vivo using fluorescence reporters in transgenic mice
Authors: Bonano VI, Oltean S, Garcia-Blanco MA.
Journal: Nat Protoc (2007): 2166
In vivo imaging of the bronchial wall microstructure using fibered confocal fluorescence microscopy
Authors: Thiberville L, Moreno-Swirc S, Vercauteren T, Peltier E, Cave C, Bourg Heckly G.
Journal: Am J Respir Crit Care Med (2007): 22
In Vivo Fluorescence Microscopic Imaging for Dynamic Quantitative Assessment of Intestinal Mucosa Permeability in Mice
Authors: Szabo A, Vollmar B, Boros M, Menger MD.
Journal: J Surg Res. (2007)
In vivo spectral fluorescence imaging of submillimeter peritoneal cancer implants using a lectin-targeted optical agent
Authors: Hama Y, Urano Y, Koyama Y, Kamiya M, Bernardo M, Paik RS, Krishna MC, Choyke PL, Kobayashi H.
Journal: Neoplasia (2006): 607
In vivo imaging of green fluorescent protein-expressing cells in transgenic animals using fibred confocal fluorescence microscopy
Authors: Al-Gubory KH, Houdebine LM.
Journal: Eur J Cell Biol (2006): 837
In vivo near-infrared fluorescence imaging of integrin alphavbeta3 in an orthotopic glioblastoma model
Authors: Hsu AR, Hou LC, Veeravagu A, Greve JM, Vogel H, Tse V, Chen X.
Journal: Mol Imaging Biol (2006): 315