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AAT Bioquest

iFluor® 450 acid

AAT Bioquest's iFluor® dyes are designed for precise protein labeling, with a focus on antibody conjugation. These dyes are characterized by their exceptional brightness, photostability, improved aqueous solubility, and minimal quenching when conjugated to proteins. They are effectively excited by key laser lines utilized in fluorescence-based instrumentation, including 350, 405, 488, 555, and 633 nm. The iFluor® 450 dye, in particular, is a 405 nm excitable fluorophore that emits green fluorescence with an emission maximum at 502 nm. Its moderate Stokes Shift, combined with stable fluorescence across a broad pH range (pH 3-11), makes it particularly well-suited for multicolor flow cytometry and confocal microscopy applications.

iFluor® 450 Key Features

  • High performance: iFluor® 450 shows brighter fluorescence than Alexa Fluor® 430 with much stronger absorption
  • Ex/Em maxima: 451/502 nm
  • Quantum yield: 0.82
  • Extinction coefficient: 40,000 cm-1M-1

iFluor® 450 acid is a non-reactive compound that can be employed as a reference standard in studies utilizing iFluor® 450 conjugates. It is also suitable for use as a control in confocal microscopy, immunocytochemistry (ICC), high-content screening (HCS), flow cytometry, and live cell imaging applications. Furthermore, it can be utilized in the synthesis of activated esters and STP and can be coupled to hydrazines, hydroxylamines, or amines in aqueous solutions using water-soluble carbodiimides (e.g., EDAC). This allows for the conjugation of the dye to amino-containing molecules, such as proteins, antibodies, amine-modified oligonucleotides, and peptides.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
iFluor® 790 acid78781225000010.1310.10.09
iFluor® 800 acid80182025000010.1110.030.08
iFluor® 810 acid81182225000010.0510.090.15
iFluor® 820 acid82285025000010.110.16
iFluor® 860 acid85387825000010.10.14
iFluor® 840 acid8368792000001-0.20.09
iFluor® 450 Styramide *Superior Replacement for Opal Polaris 480*4515024000010.8210.450.27
iFluor® 450 Tyramide *Superior Replacement for Opal 480*4515024000010.8210.450.27
iFluor® 830 acid830867----
iFluor® 670 acid67168220000010.5510.030.033
iFluor® 350 acid3454502000010.9510.830.23
iFluor® 405 acid4034273700010.9110.480.77
iFluor® 430 acid4334984000010.7810.680.3
iFluor® 647 acid65667025000010.2510.030.03
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Page updated on October 12, 2024

Ordering information

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Catalog Number2905
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Additional ordering information

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Physical properties

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.45

Correction Factor (280 nm)

0.27

Extinction coefficient (cm -1 M -1)

400001

Excitation (nm)

451

Emission (nm)

502

Quantum yield

0.821

Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
With EDAC or other equivalent activating coupling agents, fluorescent dyes, such as iFluor® 450, can react readily with the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.
With EDAC or other equivalent activating coupling agents, fluorescent dyes, such as iFluor® 450, can react readily with the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.
With EDAC or other equivalent activating coupling agents, fluorescent dyes, such as iFluor® 450, can react readily with the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.