iFluor® 450 acid

iFluor® 450 is a blue fluorescent dye similar to Alexa Fluor® 450, but it offers stronger absorption, brighter signal, and enhanced solubility.

• High performance: Brighter fluorescence and stronger absorption than Alexa Fluor® 450, with excellent photostability.
• Direct equivalent: Serves as a reliable replacement for Alexa Fluor® 450 acid, with improved brightness and chemical properties.
• Conjugation-ready chemistry: Can be converted into reactive forms for coupling to proteins, peptides, and other biomolecules.
• Broad applications: Ideal for ICC, flow cytometry, live-cell imaging, and high-content fluorescence assays.

iFluor® 450 is a blue-fluorescent dye that is excited by the 405 nm laser line and emits green fluorescence with a peak at 502 nm (Ex/Em = 451/502 nm). It exhibits stronger absorption and brighter fluorescence than Alexa Fluor® 450, along with excellent photostability, water solubility, and stable performance across a wide pH range (pH 3 to 11). With a moderate Stokes Shift and clean spectral separation, iFluor® 450 is particularly well-suited for multicolor flow cytometry and confocal microscopy.

iFluor® 450 acid is the non-reactive form of the dye and serves as a reference standard or fluorescence control in assays utilizing iFluor® 450 conjugates. It is compatible with ICC, HCS, live-cell imaging, and flow cytometry. It can also be chemically modified to generate activated esters or STP derivatives and coupled to hydrazines, hydroxylamines, or amines using water-soluble carbodiimides such as EDAC, allowing efficient labeling of proteins, antibodies, peptides, and amine-modified oligonucleotides.