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iFluor® 830 acid

iFluor® 830 is designed to label proteins and other biomolecules with infrared fluorescence. Conjugates prepared with iFluor® 830 have the excitation and emission in the IR range. iFluor® 830 dye emission is a unique color for spectrum flow cytometry as it is well separated from commonly used far-red fluorophores such as Cy5, Cy7, or allophycocyanin (APC), facilitating multicolor analysis.
iFluor® 830 is designed to label proteins and other biomolecules with infrared fluorescence. Conjugates prepared with iFluor® 830 have the excitation and emission in the IR range. iFluor® 830 dye emission is a unique color for spectrum flow cytometry as it is well separated from commonly used far-red fluorophores such as Cy5, Cy7, or allophycocyanin (APC), facilitating multicolor analysis.
Ordering information
Price ()
Catalog Number1382
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight1359.40
SolventDMSO
Spectral properties
Absorbance (nm)830
Excitation (nm)830
Emission (nm)867
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
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iFluor® 514 goat anti-mouse IgG (H+L)
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iFluor® 555 goat anti-mouse IgG (H+L)
iFluor® 594 goat anti-mouse IgG (H+L)
iFluor® 633 goat anti-mouse IgG (H+L)
iFluor® 647 goat anti-mouse IgG (H+L)
iFluor® 680 goat anti-mouse IgG (H+L)
iFluor® 700 goat anti-mouse IgG (H+L)
iFluor® 750 goat anti-mouse IgG (H+L)
iFluor® 790 goat anti-mouse IgG (H+L)
iFluor® 350 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 405 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 488 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 514 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 532 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 555 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 594 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 633 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 647 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 680 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 700 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 750 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 790 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 350 goat anti-rabbit IgG (H+L)
iFluor® 405 goat anti-rabbit IgG (H+L)
iFluor® 488 goat anti-rabbit IgG (H+L)
iFluor® 514 goat anti-rabbit IgG (H+L)
iFluor® 532 goat anti-rabbit IgG (H+L)
iFluor® 555 goat anti-rabbit IgG (H+L)
iFluor® 594 goat anti-rabbit IgG (H+L)
iFluor® 633 goat anti-rabbit IgG (H+L)
iFluor® 647 goat anti-rabbit IgG (H+L)
iFluor® 680 goat anti-rabbit IgG (H+L)
iFluor® 700 goat anti-rabbit IgG (H+L)
iFluor® 750 goat anti-rabbit IgG (H+L)
iFluor® 790 goat anti-rabbit IgG (H+L)
iFluor® 350 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 405 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 488 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 514 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 532 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 555 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 594 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 633 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 647 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 680 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 700 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 750 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 790 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 350-streptavidin conjugate
iFluor® 405-streptavidin conjugate
iFluor® 488-streptavidin conjugate
iFluor® 514-streptavidin conjugate
iFluor® 532-streptavidin conjugate
iFluor® 555-streptavidin conjugate
iFluor® 594-streptavidin conjugate
iFluor® 633-streptavidin conjugate
iFluor® 647-streptavidin conjugate
iFluor® 680-streptavidin conjugate
iFluor® 700-streptavidin conjugate
iFluor® 750-streptavidin conjugate
iFluor 750™ RGD Conjugate
iFluor® 488 azide
iFluor® 405 hydrazide
iFluor® 800 maleimide
iFluor® 810 maleimide
iFluor® 820 maleimide
iFluor® 860 maleimide
iFluor® 546 goat anti-mouse IgG (H+L)
iFluor® 546 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 568 goat anti-mouse IgG (H+L)
iFluor® 568 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 546 goat anti-rabbit IgG (H+L)
iFluor® 546 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 568 goat anti-rabbit IgG (H+L)
iFluor® 568 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 555 alkyne
iFluor® 555 azide
iFluor® 546-streptavidin conjugate
iFluor® 568-streptavidin conjugate
iFluor® 532 maleimide
iFluor® 594 maleimide
iFluor® 488 alkyne
iFluor® 405 maleimide
iFluor® 430 maleimide
iFluor® 568 maleimide
iFluor® 633 maleimide
iFluor® 450 maleimide
iFluor® 350 Styramide *Superior Replacement for Alexa Fluor 350 tyramide*
iFluor® 488 Styramide *Superior Replacement for Alexa Fluor 488 tyramide and Opal 520*
iFluor® 546 Styramide *Superior Replacement for Alexa Fluor 546 tyramide*
iFluor® 555 Styramide *Superior Replacement for Alexa Fluor 555 tyramide and Opal 570*
iFluor® 568 Styramide *Superior Replacement for Alexa Fluor 568 tyramide*
iFluor® 594 Styramide *Superior Replacement for Alexa Fluor 594 tyramide*
iFluor® 647 Styramide *Superior Replacement for Alexa Fluor 647 tyramide*
iFluor® 680 Styramide *Superior Replacement for Alexa Fluor 680 tyramide and Opal 690*
iFluor® 700 Styramide *Superior Replacement for Alexa Fluor 700 tyramide*
iFluor® 750 Styramide *Superior Replacement for Alexa Fluor 750 tyramide*
iFluor® 790 Styramide *Superior Replacement for Alexa Fluor 790 tyramide*
iFluor® 555 Tyramide
iFluor® 647 Tyramide
iFluor® 350 PSA™ Imaging Kit with Goat Anti-Rabbit IgG
iFluor® 488 PSA™ Imaging Kit with Goat Anti-Rabbit IgG
iFluor® 555 PSA™ Imaging Kit with Goat Anti-Rabbit IgG
iFluor® 594 PSA™ Imaging Kit with Goat Anti-Rabbit IgG
iFluor® 647 PSA™ Imaging Kit with Goat Anti-Rabbit IgG
iFluor 350™ PSA™ Imaging Kit with Goat Anti-Mouse IgG
iFluor 488™ PSA™ Imaging Kit with Goat Anti-Mouse IgG
iFluor 555™ PSA™ Imaging Kit with Goat Anti-Mouse IgG
iFluor 594™ PSA™ Imaging Kit with Goat Anti-Mouse IgG
iFluor 647™ PSA™ Imaging Kit with Goat Anti-Mouse IgG
iFluor® 790-streptavidin conjugate
iFluor® 800-streptavidin conjugate
iFluor® 820-streptavidin conjugate
iFluor® 840-streptavidin conjugate
iFluor® 860-streptavidin conjugate
iFluor® 488-Wheat Germ Agglutinin (WGA) Conjugate
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iFluor® 488-Concanavalin A Conjugate
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iFluor® 594-Concanavalin A Conjugate
iFluor® 647-Concanavalin A Conjugate
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iFluor® 594 Tyramide
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iFluor® 555 TCO
iFluor® 594 TCO
iFluor® 647 TCO
iFluor® 488 Tetrazine
iFluor® 555 Tetrazine
iFluor® 594 Tetrazine
iFluor® 647 Tetrazine
iFluor® 440-dUTP *1 mM in Tris Buffer (pH 7.5)*
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iFluor® 514 Styramide *Superior Replacement for Opal 540*
iFluor® 532 Styramide
iFluor® 633 Styramide *Superior Replacement for Opal 650*
iFluor® 440 Styramide
iFluor® 460 Styramide
iFluor® 610 Styramide
iFluor® 660 Styramide
iFluor® 405 Styramide
iFluor®488-PEG12-dUTP *1 mM in Tris Buffer (pH 7.5)*
iFluor®555-PEG12-dUTP *1 mM in Tris Buffer (pH 7.5)*
iFluor®647-PEG12-dUTP *1 mM in Tris Buffer (pH 7.5)*
iFluor® 800 goat anti-mouse IgG (H+L)
iFluor® 800 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 810 goat anti-mouse IgG (H+L)
iFluor® 810 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 820 goat anti-mouse IgG (H+L)
iFluor® 820 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 840 goat anti-mouse IgG (H+L)
iFluor® 840 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 860 goat anti-mouse IgG (H+L)
iFluor® 860 goat anti-mouse IgG (H+L) *Cross Adsorbed*
iFluor® 800 goat anti-rabbit IgG (H+L)
iFluor® 800 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 810 goat anti-rabbit IgG (H+L)
iFluor® 810 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 820 goat anti-rabbit IgG (H+L)
iFluor® 820 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 840 goat anti-rabbit IgG (H+L)
iFluor® 840 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 860 goat anti-rabbit IgG (H+L)
iFluor® 860 goat anti-rabbit IgG (H+L) *Cross Adsorbed*
iFluor® 430 Tyramide *Superior Replacement for Opal 480*
iFluor® 450 Tyramide *Superior Replacement for Opal 480*
iFluor® 546 maleimide
iFluor® 840 maleimide
iFluor® 770 maleimide
iFluor® 780 maleimide
iFluor® 350 succinimidyl ester
iFluor® 405 succinimidyl ester
iFluor® 488 succinimidyl ester
iFluor® 514 succinimidyl ester
iFluor® 532 succinimidyl ester
iFluor® 555 succinimidyl ester
iFluor® 594 succinimidyl ester
iFluor® 633 succinimidyl ester
iFluor® 647 succinimidyl ester
iFluor® 660 succinimidyl ester
iFluor® 680 succinimidyl ester
iFluor® 700 succinimidyl ester
iFluor® 750 succinimidyl ester
iFluor® 610 succinimidyl ester
iFluor® 710 succinimidyl ester
iFluor® 790 succinimidyl ester
iFluor® 800 succinimidyl ester
iFluor® 810 succinimidyl ester
iFluor® 820 succinimidyl ester
iFluor® 860 succinimidyl ester
iFluor® 546 succinimidyl ester
iFluor® 568 succinimidyl ester
iFluor® 430 succinimidyl ester
iFluor® 450 succinimidyl ester
iFluor® 840 succinimidyl ester
iFluor® 560 succinimidyl ester
iFluor® 670 succinimidyl ester
iFluor® 460 succinimidyl ester
iFluor® 440 succinimidyl ester
iFluor® 665 succinimidyl ester
iFluor® 690 succinimidyl ester
iFluor® Ultra 594 succinimidyl ester
iFluor® Ultra 647 succinimidyl ester
iFluor® Ultra 750 succinimidyl ester
iFluor® 720 succinimidyl ester
iFluor® 740 succinimidyl ester
iFluor® 597 succinimidyl ester
iFluor® 770 succinimidyl ester
iFluor® 780 succinimidyl ester
iFluor® 570 succinimidyl ester
iFluor™ 405 azide
iFluor® 514 maleimide
iFluor® 660 maleimide
iFluor® 670 maleimide
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OverviewpdfSDSpdfProtocol


Molecular weight
1359.40
Absorbance (nm)
830
Excitation (nm)
830
Emission (nm)
867
In vivo fluorescence imaging uses a sensitive camera to detect the fluorescence emission from fluorophores in whole-body living small animals. To overcome the photon attenuation in living tissue, fluorophores with long emissions at the infrared (IR) region are generally preferred. Recent advances in imaging strategies and reporter techniques for in vivo fluorescence imaging include novel approaches to improve the specificity and affinity of the probes and to modulate and amplify the signal at target sites for enhanced sensitivity. Further developments aim to achieve high-resolution, multimodality, and lifetime-based in vivo fluorescence imaging. Our iFluor® 830 is designed to label proteins and other biomolecules with infrared fluorescence. Conjugates prepared with iFluor® 830 have excitation and emission in the IR range. iFluor® 830 dye emission is a unique color for spectrum flow cytometry as it is well separated from commonly used far-red fluorophores such as Cy5, Cy7, or allophycocyanin (APC), facilitating multicolor analysis. This fluorophore is also useful for small animal in vivo imaging applications or other imaging applications requiring IR detection.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 830 acid to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM73.562 µL367.809 µL735.619 µL3.678 mL7.356 mL
5 mM14.712 µL73.562 µL147.124 µL735.619 µL1.471 mL
10 mM7.356 µL36.781 µL73.562 µL367.809 µL735.619 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

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Spectrum


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spectrum

Spectral properties

Absorbance (nm)830
Excitation (nm)830
Emission (nm)867

Product family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
iFluor® 790 acid78781225000010.1310.10.09
iFluor® 800 acid80182025000010.1110.030.08
iFluor® 810 acid81182225000010.0510.090.15
iFluor® 820 acid8228502500001-0.110.16
iFluor® 860 acid8538782500001-0.10.14
iFluor® 840 acid8368792000001-0.20.09

References


View all 50 references: Citation Explorer
Intraoperative indocyanine green (ICG) angiography of the parathyroids glands in prediction of post-thyroidectomy hypocalcemia: Diagnostic accuracy of the ICG score 2 versus the 4-ICG score.
Authors: Moreno Llorente, Pablo and García Barrasa, Arantxa and Francos Martínez, José Manuel and Alberich Prats, Marta and Pascua Solé, Mireia
Journal: Cirugia espanola (2022): 274-280
Head and neck pedicled flap autonomization using a new high-resolution indocyanine green fluorescence video-angiography device.
Authors: Giordano, Leone and Galli, Andrea and Familiari, Marco and Canta, Davide and Irem, Ayhan and Biafora, Matteo and Battista, Rosa Alessia and Bussi, Mario
Journal: Head & neck (2022): 1496-1499
Indocyanine green fluorescence image processing techniques for breast cancer macroscopic demarcation.
Authors: Leiloglou, Maria and Kedrzycki, Martha S and Chalau, Vadzim and Chiarini, Nicolas and Thiruchelvam, Paul T R and Hadjiminas, Dimitri J and Hogben, Katy R and Rashid, Faiza and Ramakrishnan, Rathi and Darzi, Ara W and Leff, Daniel R and Elson, Daniel S
Journal: Scientific reports (2022): 8607
A phase III, multicenter, single-arm study to assess the utility of indocyanine green fluorescent lymphography in the treatment of secondary lymphedema.
Authors: Akita, Shinsuke and Unno, Naoki and Maegawa, Jiro and Kimata, Yoshihiro and Fukamizu, Hidekazu and Yabuki, Yuichiro and Kitayama, Shinya and Shinaoka, Akira and Yamada, Kiyoshi and Sano, Masaki and Ota, Yusuke and Ohnishi, Fumio and Sakuma, Hisashi and Nuri, Takashi and Ozawa, Yoshihito and Shiko, Yuki and Kawasaki, Yohei and Hanawa, Michiko and Fujii, Yasuhisa and Imanishi, Eri and Fujiwara, Tadami and Hanaoka, Hideki and Mitsukawa, Nobuyuki
Journal: Journal of vascular surgery. Venous and lymphatic disorders (2022): 728-737.e3
Indocyanine Green Angiography of Parathyroid Glands versus Intraoperative Parathyroid Hormone Assay as a Reliable Predictor for Post Thyroidectomy Transient Hypocalcemia.
Authors: Abdelrahim, Hossam S and Amer, Ahmed F and Mikhael Nageeb, Ramy
Journal: Journal of investigative surgery : the official journal of the Academy of Surgical Research (2022): 1-8
Cone-beam CT-guided tagging of endophytic renal tumors with indocyanine green via trans-arterial selective delivery in patients undergoing robot-assisted partial nephrectomy with near infrared fluorescence imaging.
Authors: Nardis, Pier Giorgio and Cipollari, Stefano and Lucatelli, Pierleone and Basilico, Fabrizio and Rocco, Bianca and Corona, Mario and Cannavale, Alessandro and Leonardo, Costantino and Flammia, Rocco Simone and Proietti, Flavia and Vallati, Giulio and Gallucci, Michele and Catalano, Carlo
Journal: Journal of vascular and interventional radiology : JVIR (2022)
Using indocyanine green fluorescence in laparoscopic surgery to identify and preserve rare branching of the right hepatic artery in pediatric congenital biliary dilatation.
Authors: Masuya, Ryuta and Matsukubo, Makoto and Nakame, Kazuhiko and Kai, Kengo and Hamada, Takeomi and Yano, Koichi and Imamura, Naoya and Hiyoshi, Masahide and Nanashima, Atsushi and Ieiri, Satoshi
Journal: Surgery today (2022)
Robotic-assisted Laparoscopic Ureterolysis for Deep Infiltrating Endometriosis using Indocyanine Green Under Near-Infrared Fluorescence.
Authors: Thigpen, Brooke and Koythong, Tamisa and Guan, Xiaoming
Journal: Journal of minimally invasive gynecology (2022): 586-587
ASO Visual Abstract: A Prospective Study Evaluating the Accuracy of Indocyanine Green (ICG) Fluorescence Compared with Radioisotope for Sentinel Lymph Node (Sln) Detection in Early Breast Cancer.
Authors: Dumitru, Dorin and Ghanakumar, Sujit and Provenzano, Elena and Benson, John R
Journal: Annals of surgical oncology (2022): 3021
Second Window Indocyanine Green for Oropharyngeal Tumors: A Case Series and Comparison of Near-Infrared Camera Systems.
Authors: De Ravin, Emma and Carey, Ryan M and Stubbs, Vanessa C and Jaffe, Samantha and Lee, John Y K and Rajasekaran, Karthik and Newman, Jason G
Journal: Clinical otolaryngology : official journal of ENT-UK ; official journal of Netherlands Society for Oto-Rhino-Laryngology & Cervi (2022)