iFluor® 830 acid
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1359.40 |
Solvent | DMSO |
Spectral properties
Absorbance (nm) | 830 |
Excitation (nm) | 830 |
Emission (nm) | 867 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
iFluor® 830 succinimidyl ester |
iFluor® 830 maleimide |
Overview | ![]() ![]() |
See also: iFluor® Dyes and Kits
Molecular weight 1359.40 | Absorbance (nm) 830 | Excitation (nm) 830 | Emission (nm) 867 |
In vivo fluorescence imaging uses a sensitive camera to detect the fluorescence emission from fluorophores in whole-body living small animals. To overcome the photon attenuation in living tissue, fluorophores with long emissions at the infrared (IR) region are generally preferred. Recent advances in imaging strategies and reporter techniques for in vivo fluorescence imaging include novel approaches to improve the specificity and affinity of the probes and to modulate and amplify the signal at target sites for enhanced sensitivity. Further developments aim to achieve high-resolution, multimodality, and lifetime-based in vivo fluorescence imaging. Our iFluor® 830 is designed to label proteins and other biomolecules with infrared fluorescence. Conjugates prepared with iFluor® 830 have excitation and emission in the IR range. iFluor® 830 dye emission is a unique color for spectrum flow cytometry as it is well separated from commonly used far-red fluorophores such as Cy5, Cy7, or allophycocyanin (APC), facilitating multicolor analysis. This fluorophore is also useful for small animal in vivo imaging applications or other imaging applications requiring IR detection.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 830 acid to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 73.562 µL | 367.809 µL | 735.619 µL | 3.678 mL | 7.356 mL |
5 mM | 14.712 µL | 73.562 µL | 147.124 µL | 735.619 µL | 1.471 mL |
10 mM | 7.356 µL | 36.781 µL | 73.562 µL | 367.809 µL | 735.619 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
Open in Advanced Spectrum Viewer


Spectral properties
Absorbance (nm) | 830 |
Excitation (nm) | 830 |
Emission (nm) | 867 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
iFluor® 790 acid | 787 | 812 | 2500001 | 0.131 | 0.1 | 0.09 |
iFluor® 800 acid | 801 | 820 | 2500001 | 0.111 | 0.03 | 0.08 |
iFluor® 810 acid | 811 | 822 | 2500001 | 0.051 | 0.09 | 0.15 |
iFluor® 820 acid | 822 | 850 | 2500001 | 0.11 | 0.16 | |
iFluor® 860 acid | 853 | 878 | 2500001 | 0.1 | 0.14 | |
iFluor® 840 acid | 836 | 879 | 2000001 | - | 0.2 | 0.09 |
Images

Figure 1. iFluor® 830 is designed to label proteins and other biomolecules with infrared fluorescence. Conjugates prepared with iFluor® 830 have the excitation and emission in the IR range. iFluor® 830 dye emission is a unique color for spectrum flow cytometry as it is well separated from commonly used far-red fluorophores such as Cy5, Cy7, or allophycocyanin (APC), facilitating multicolor analysis.
References
View all 50 references: Citation Explorer
Intraoperative indocyanine green (ICG) angiography of the parathyroids glands in prediction of post-thyroidectomy hypocalcemia: Diagnostic accuracy of the ICG score 2 versus the 4-ICG score.
Authors: Moreno Llorente, Pablo and García Barrasa, Arantxa and Francos Martínez, José Manuel and Alberich Prats, Marta and Pascua Solé, Mireia
Journal: Cirugia espanola (2022): 274-280
Authors: Moreno Llorente, Pablo and García Barrasa, Arantxa and Francos Martínez, José Manuel and Alberich Prats, Marta and Pascua Solé, Mireia
Journal: Cirugia espanola (2022): 274-280
Head and neck pedicled flap autonomization using a new high-resolution indocyanine green fluorescence video-angiography device.
Authors: Giordano, Leone and Galli, Andrea and Familiari, Marco and Canta, Davide and Irem, Ayhan and Biafora, Matteo and Battista, Rosa Alessia and Bussi, Mario
Journal: Head & neck (2022): 1496-1499
Authors: Giordano, Leone and Galli, Andrea and Familiari, Marco and Canta, Davide and Irem, Ayhan and Biafora, Matteo and Battista, Rosa Alessia and Bussi, Mario
Journal: Head & neck (2022): 1496-1499
Indocyanine green fluorescence image processing techniques for breast cancer macroscopic demarcation.
Authors: Leiloglou, Maria and Kedrzycki, Martha S and Chalau, Vadzim and Chiarini, Nicolas and Thiruchelvam, Paul T R and Hadjiminas, Dimitri J and Hogben, Katy R and Rashid, Faiza and Ramakrishnan, Rathi and Darzi, Ara W and Leff, Daniel R and Elson, Daniel S
Journal: Scientific reports (2022): 8607
Authors: Leiloglou, Maria and Kedrzycki, Martha S and Chalau, Vadzim and Chiarini, Nicolas and Thiruchelvam, Paul T R and Hadjiminas, Dimitri J and Hogben, Katy R and Rashid, Faiza and Ramakrishnan, Rathi and Darzi, Ara W and Leff, Daniel R and Elson, Daniel S
Journal: Scientific reports (2022): 8607
A phase III, multicenter, single-arm study to assess the utility of indocyanine green fluorescent lymphography in the treatment of secondary lymphedema.
Authors: Akita, Shinsuke and Unno, Naoki and Maegawa, Jiro and Kimata, Yoshihiro and Fukamizu, Hidekazu and Yabuki, Yuichiro and Kitayama, Shinya and Shinaoka, Akira and Yamada, Kiyoshi and Sano, Masaki and Ota, Yusuke and Ohnishi, Fumio and Sakuma, Hisashi and Nuri, Takashi and Ozawa, Yoshihito and Shiko, Yuki and Kawasaki, Yohei and Hanawa, Michiko and Fujii, Yasuhisa and Imanishi, Eri and Fujiwara, Tadami and Hanaoka, Hideki and Mitsukawa, Nobuyuki
Journal: Journal of vascular surgery. Venous and lymphatic disorders (2022): 728-737.e3
Authors: Akita, Shinsuke and Unno, Naoki and Maegawa, Jiro and Kimata, Yoshihiro and Fukamizu, Hidekazu and Yabuki, Yuichiro and Kitayama, Shinya and Shinaoka, Akira and Yamada, Kiyoshi and Sano, Masaki and Ota, Yusuke and Ohnishi, Fumio and Sakuma, Hisashi and Nuri, Takashi and Ozawa, Yoshihito and Shiko, Yuki and Kawasaki, Yohei and Hanawa, Michiko and Fujii, Yasuhisa and Imanishi, Eri and Fujiwara, Tadami and Hanaoka, Hideki and Mitsukawa, Nobuyuki
Journal: Journal of vascular surgery. Venous and lymphatic disorders (2022): 728-737.e3
Indocyanine Green Angiography of Parathyroid Glands versus Intraoperative Parathyroid Hormone Assay as a Reliable Predictor for Post Thyroidectomy Transient Hypocalcemia.
Authors: Abdelrahim, Hossam S and Amer, Ahmed F and Mikhael Nageeb, Ramy
Journal: Journal of investigative surgery : the official journal of the Academy of Surgical Research (2022): 1-8
Authors: Abdelrahim, Hossam S and Amer, Ahmed F and Mikhael Nageeb, Ramy
Journal: Journal of investigative surgery : the official journal of the Academy of Surgical Research (2022): 1-8
Cone-beam CT-guided tagging of endophytic renal tumors with indocyanine green via trans-arterial selective delivery in patients undergoing robot-assisted partial nephrectomy with near infrared fluorescence imaging.
Authors: Nardis, Pier Giorgio and Cipollari, Stefano and Lucatelli, Pierleone and Basilico, Fabrizio and Rocco, Bianca and Corona, Mario and Cannavale, Alessandro and Leonardo, Costantino and Flammia, Rocco Simone and Proietti, Flavia and Vallati, Giulio and Gallucci, Michele and Catalano, Carlo
Journal: Journal of vascular and interventional radiology : JVIR (2022)
Authors: Nardis, Pier Giorgio and Cipollari, Stefano and Lucatelli, Pierleone and Basilico, Fabrizio and Rocco, Bianca and Corona, Mario and Cannavale, Alessandro and Leonardo, Costantino and Flammia, Rocco Simone and Proietti, Flavia and Vallati, Giulio and Gallucci, Michele and Catalano, Carlo
Journal: Journal of vascular and interventional radiology : JVIR (2022)
Using indocyanine green fluorescence in laparoscopic surgery to identify and preserve rare branching of the right hepatic artery in pediatric congenital biliary dilatation.
Authors: Masuya, Ryuta and Matsukubo, Makoto and Nakame, Kazuhiko and Kai, Kengo and Hamada, Takeomi and Yano, Koichi and Imamura, Naoya and Hiyoshi, Masahide and Nanashima, Atsushi and Ieiri, Satoshi
Journal: Surgery today (2022)
Authors: Masuya, Ryuta and Matsukubo, Makoto and Nakame, Kazuhiko and Kai, Kengo and Hamada, Takeomi and Yano, Koichi and Imamura, Naoya and Hiyoshi, Masahide and Nanashima, Atsushi and Ieiri, Satoshi
Journal: Surgery today (2022)
Robotic-assisted Laparoscopic Ureterolysis for Deep Infiltrating Endometriosis using Indocyanine Green Under Near-Infrared Fluorescence.
Authors: Thigpen, Brooke and Koythong, Tamisa and Guan, Xiaoming
Journal: Journal of minimally invasive gynecology (2022): 586-587
Authors: Thigpen, Brooke and Koythong, Tamisa and Guan, Xiaoming
Journal: Journal of minimally invasive gynecology (2022): 586-587
ASO Visual Abstract: A Prospective Study Evaluating the Accuracy of Indocyanine Green (ICG) Fluorescence Compared with Radioisotope for Sentinel Lymph Node (Sln) Detection in Early Breast Cancer.
Authors: Dumitru, Dorin and Ghanakumar, Sujit and Provenzano, Elena and Benson, John R
Journal: Annals of surgical oncology (2022): 3021
Authors: Dumitru, Dorin and Ghanakumar, Sujit and Provenzano, Elena and Benson, John R
Journal: Annals of surgical oncology (2022): 3021
Second Window Indocyanine Green for Oropharyngeal Tumors: A Case Series and Comparison of Near-Infrared Camera Systems.
Authors: De Ravin, Emma and Carey, Ryan M and Stubbs, Vanessa C and Jaffe, Samantha and Lee, John Y K and Rajasekaran, Karthik and Newman, Jason G
Journal: Clinical otolaryngology : official journal of ENT-UK ; official journal of Netherlands Society for Oto-Rhino-Laryngology & Cervi (2022)
Authors: De Ravin, Emma and Carey, Ryan M and Stubbs, Vanessa C and Jaffe, Samantha and Lee, John Y K and Rajasekaran, Karthik and Newman, Jason G
Journal: Clinical otolaryngology : official journal of ENT-UK ; official journal of Netherlands Society for Oto-Rhino-Laryngology & Cervi (2022)