HIS Lite™ iFluor® 568 Tris NTA Chelator

HIS Lite™ iFluor® 568 Tris NTA Chelator is a fluorescent probe for selective, noncovalent labeling and detection of polyhistidine-tagged proteins in cells, solutions, and on solid surfaces.

Selective Labeling: Tris-NTA chelator targets polyhistidine-tagged proteins through metal-mediated binding.
Spectral Properties: Excitation at 568 nm and emission at 587 nm.
Stable Binding: Multivalent tris-NTA interactions provide greater binding stability than mono-NTA.
Multicolor Compatibility: Can be combined with OG488-tris-NTA for multicolor protein analysis.
Applications: Suitable for protein detection, interaction studies, and cell-surface labeling.

iFluor® 565 Tris-NTA Chelator is used as a sensitive fluorescent probe for detecting polyhistidine-labeled proteins in combination with the addition of a heavy metal ion( such as Ni2+) in cells, solution and solid surfaces.

Datasheet

SDS

COA

Catalog	Size	Price	Quantity
12657	100 ug	Price

Overview

iFluor® 568 Tris-NTA Chelator is used as a sensitive fluorescent probe for detecting polyhistidine-labeled proteins in combination with the addition of a heavy metal ion( such as Ni2+) in cells, solution and solid surfaces. Fluorescent tris-NTA compounds provide an efficient method for site-specific and stable noncovalent fluorescence labeling of polyhistidine-tagged proteins. In combination with OG488-tris-NTA compound iFluor® 568 Tris-NTA can be used for multicolor analysis of polyhistidine-tagged proteins. In contrast to the transient binding of conventional mono-NTA, the multivalent interaction of tris-NTA conjugated fluorophores form a much more stable complex with polyhistidine-tagged proteins. The high selectivity of tris-NTA compounds toward cumulated histidines enables the selective labeling of proteins in cell lysates and on the surface of live cells. Fluorescent tris-NTA conjugates can be applied for the analysis of a ternary protein complex in solution and on surfaces. AAT Bioquest also offers HIS Lite™ iFluor® 568 Tris NTA-Ni Complex that can be directly used for the specific and highly sensitive detection of His-tagged fusion proteins without the addition of a heavy metal ion. The transition metal ions (e.g., Ni ion)-mediated complexation of polyhistidine-labeled proteins with fluorescent tris-NTA conjugates provides a sensitive reporter for detecting and monitoring protein-protein interactions in real time.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of HIS Lite™ iFluor® 568 Tris NTA Chelator to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	64.866 µL	324.332 µL	648.664 µL	3.243 mL	6.487 mL
5 mM	12.973 µL	64.866 µL	129.733 µL	648.664 µL	1.297 mL
10 mM	6.487 µL	32.433 µL	64.866 µL	324.332 µL	648.664 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1541.63 g/mol	=	mL	x	mM	=	mmol

References

View all 33 references: Citation Explorer

Engineering Protein Nanoparticles Functionalized with an Immunodominant Coxiella burnetii Antigen to Generate a Q Fever Vaccine.

Authors:

Ramirez, Aaron and Felgner, Jiin and Jain, Aarti and Jan, Sharon and Albin, Tyler J and Badten, Alexander J and Gregory, Anthony E and Nakajima, Rie and Jasinskas, Algimantas and Felgner, Philip L and Burkhardt, Amanda M and Davies, D Huw and Wang, Szu-Wen

Journal:

Bioconjugate chemistry (2023): 1653-1666

Protein Nanoparticle-Mediated Delivery of Recombinant Influenza Hemagglutinin Enhances Immunogenicity and Breadth of the Antibody Response.

Authors:

Badten, Alexander J and Ramirez, Aaron and Hernandez-Davies, Jenny E and Albin, Tyler J and Jain, Aarti and Nakajima, Rie and Felgner, Jiin and Davies, D Huw and Wang, Szu-Wen

Journal:

ACS infectious diseases (2023): 239-252

A Four-Channel Surface Plasmon Resonance Sensor Functionalized Online for Simultaneous Detections of Anti-SARS-CoV-2 Antibody, Free Viral Particles, and Neutralized Viral Particles.

Authors:

Dong, Tianbao and Han, Chaowei and Jiang, Meng and Zhang, Tiantian and Kang, Qing and Wang, Pengcheng and Zhou, Feimeng

Journal:

ACS sensors (2022): 3560-3570

Regenerable and high-throughput surface plasmon resonance assay for rapid screening of anti-SARS-CoV-2 antibody in serum samples.

Authors:

Jiang, Meng and Dong, Tianbao and Han, Chaowei and Liu, Luyao and Zhang, Tiantian and Kang, Qing and Wang, Pengcheng and Zhou, Feimeng

Journal:

Analytica chimica acta (2022): 339830

Facile Method of Tubulin Purification from Goat Brain for Reconstitution of Microtubule-Associated Intracellular Function.

Authors:

Ghosh, Satyajit and Garg, Shubham and Mukherjee, Nabanita and Ghosh, Surajit

Journal:

Methods in molecular biology (Clifton, N.J.) (2022): 17-45

Physical properties

Molecular weight	1541.63
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.34
Correction factor (280 nm)	0.15
Extinction coefficient (cm ^-1 M ^-1)	100000 ¹
Excitation (nm)	568
Emission (nm)	587
Quantum yield	0.57 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on October 26, 2026