Deoxycholic acid-iFluor® 647 conjugate

Deoxycholic acid, also known as cholanoic acid is a bile acid. Deoxycholic acid-iFluor® 647 conjugate is a red fluorescent derivative of deoxycholic acid. It is an iFluor® 647-labeled bile acid derivative that closely parallels the cellular binding and uptake properties of deoxycholic acid. It is a divalent 'unipolar' anionic fluorescent bile salt analog that might offer a potentially useful probe for studying cellular mechanisms of deoxycholic acid at the hepatocellular level. Deoxycholic acid-iFluor® 647 conjugate can be used as a tool for the visualization of bile acid transport for the samples that have either strong blue or green fluorescence since its strong red fluorescence is well separated from blue or green fluorescence. Deoxycholic acid is one of the secondary bile acids, which are metabolic byproducts of intestinal bacteria. The two primary bile acids secreted by the liver are cholic acid and chenodeoxycholic acid. Deoxycholic acid, under the brand name Kybella, is approved by the FDA for reducing moderate-to-severe fat below the chin. When injected into submental fat, deoxycholic acid helps destroy fat cells.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
36705	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Deoxycholic acid-iFluor® 647 conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	79.768 µL	398.842 µL	797.684 µL	3.988 mL	7.977 mL
5 mM	15.954 µL	79.768 µL	159.537 µL	797.684 µL	1.595 mL
10 mM	7.977 µL	39.884 µL	79.768 µL	398.842 µL	797.684 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1253.63 g/mol	=	mL	x	mM	=	mmol

References

View all 50 references: Citation Explorer

Cationic nanoparticles self-assembled from amphiphilic chitosan derivatives containing poly(amidoamine) dendrons and deoxycholic acid as a vector for co-delivery of doxorubicin and gene.

Authors:

Chen, Shanshan and Deng, Junjie and Zhang, Li-Ming

Journal:

Carbohydrate polymers (2021): 117706

Deoxycholic Acid and Lithocholic Acid Alleviate Liver Injury and Inflammation in Mice with Klebsiella pneumoniae-Induced Liver Abscess and Bacteremia.

Authors:

Zheng, Yahong and Yue, Chengcheng and Zhang, Hui and Chen, Haoran and Liu, Yanyan and Li, Jiabin

Journal:

Journal of inflammation research (2021): 777-789

Efficacy and safety of injectable deoxycholic acid for submental fat reduction: a systematic review and meta-analysis of randomized controlled trials.

Authors:

Cunha, Karin Soares and Lima, Flávia and Cardoso, Roberta Marques

Journal:

Expert review of clinical pharmacology (2021): 383-397

Free Deoxycholic Acid Exacerbates Vascular Calcification in CKD through ER Stress-Mediated ATF4 Activation.

Authors:

Miyazaki-Anzai, Shinobu and Masuda, Masashi and Shiozaki, Yuji and Keenan, Audrey L and Chonchol, Michel and Kremoser, Claus and Miyazaki, Makoto

Journal:

Kidney360 (2021): 857-868

pH-sensitive chitosan-deoxycholic acid/alginate nanoparticles for oral insulin delivery.

Authors:

Zhang, Ya-Wen and Tu, Ling-Lan and Tang, Zhan and Wang, Qiao and Zheng, Gao-Li and Yin, Li-Na

Journal:

Pharmaceutical development and technology (2021): 1-10

Physical properties

Molecular weight	1253.63
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.03
Correction factor (280 nm)	0.03
Correction factor (656 nm)	0.0793
Extinction coefficient (cm ^-1 M ^-1)	250000 ¹
Excitation (nm)	656
Emission (nm)	670
Quantum yield	0.25 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on November 23, 2025