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iFluor® 594 goat anti-rabbit IgG (H+L)
AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). iFluor® 594 goat anti-rabbit IgG (H+L) conjugate has fluorescence excitation and emission maxima of ~592 nm and ~614 nm respectively. These spectral characteristics make them an excellent alternative to Alexa Fluor® 594 goat anti-rabbit IgG (H+L) conjugate (Alexa Fluor® is the trademark of Invitrogen).
<p>HeLa cells were stained with rabbit anti-tubulin followed with iFluor<sup>TM</sup> 594 goat anti-rabbit IgG (H+L) (red); actin filaments were stained with Phalloidin-iFluor<sup>TM</sup> 488 conjugate (green); and nuclei were stained with DAPI (blue).</p>
<p>HeLa cells were stained with rabbit anti-tubulin followed with iFluor<sup>TM</sup> 594 goat anti-rabbit IgG (H+L) (red); actin filaments were stained with Phalloidin-iFluor<sup>TM</sup> 488 conjugate (green); and nuclei were stained with DAPI (blue).</p>
protocol
Protocol
COA
CatalogSize
Price
Quantity
16628200 ug
Price
168061 mg
Price
 
Physical properties
Molecular weight~150000
SolventWater
Spectral properties
Absorbance (nm)587
Correction factor (260 nm)0.05
Correction factor (280 nm)0.04
Extinction coefficient (cm -1 M -1)
200000
1
Excitation (nm)587
Emission (nm)603
Quantum yield
0.53
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Page updated on October 8, 2025