iFluor® 680 Styramide™

iFluor® 680 Styramide™ is a far-red fluorescent signal amplification reagent designed for sensitive detection of low-abundance targets in cells and tissues using HRP-mediated labeling.

Detection Principle: Uses HRP-catalyzed covalent deposition of fluorescent labels.
High Sensitivity: Generates 10–50 times stronger fluorescence signals than conventional tyramide reagents.
Instrument Compatibility: Compatible with Cy5 fluorescence microscope filter sets.
Applications: Suitable for immunocytochemistry, immunofluorescence, and immunohistochemistry.

Formalin-fixed, paraffin-embedded (FFPE) human lung tissue was labeled with anti-EpCAM mouse mAb followed by HRP-labeled goat anti-mouse IgG (Cat No. 16728). The fluorescence signal was developed using iFluor® 680 styramide (Cat No. 45050) and detected with a Cy5 filter set.

Protocol

SDS

Catalog	Size	Price	Quantity
45050	100 Slides	Price

Overview

Power Styramide™ Signal Amplification (PSA™) system is one of the most sensitive methods that can detect extremely low-abundance targets in cells and tissues with improved fluorescence signal 10-50 times higher than the widely used tyramide (TSA) reagents. In combination with our superior iFluor® dyes that have higher florescence intensity, increased photostability and enhanced water solubility, the iFluor® dye-labeled Styramide™ conjugates can generate fluorescence signal with significantly higher precision and sensitivity (more than 100 times) than standard ICC/IF/IHC. PSA™ utilizes the catalytic activity of horseradish peroxidase (HRP) for covalent deposition of fluorophores in situ. PSA™ radicals have much higher reactivity than tyramide radicals, making the PSA™ system much faster, more robust and sensitive than the traditional TSA reagents. Compared to tyramide reagents, the Styramide™ conjugates have ability to label the target at higher efficiency and thus generate significantly higher fluorescence signal. Styramide™ conjugates also allow significantly less consumption of primary antibody compared to standard directly conjugate method or tyramide amplification with the same level of sensitivity. iFluor® 680 Styramide is a superior replacement for Alexa Fluor 680 tyramide, Opal 690 or other spectrally similar fluorescent tyramide conjugates or TSA reagents.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 680 Styramide™ to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	86.86 µL	434.299 µL	868.598 µL	4.343 mL	8.686 mL
5 mM	17.372 µL	86.86 µL	173.72 µL	868.598 µL	1.737 mL
10 mM	8.686 µL	43.43 µL	86.86 µL	434.299 µL	868.598 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1151.28 g/mol	=	mL	x	mM	=	mmol

Physical properties

Molecular weight	1151.28
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.097
Correction factor (280 nm)	0.094
Extinction coefficient (cm ^-1 M ^-1)	220000 ¹
Excitation (nm)	684
Emission (nm)	701
Quantum yield	0.23 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

Fluorescence microscope
Excitation	Cy5 filter set
Emission	Cy5 filter set
Recommended plate	Black wall/clear bottom

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Page updated on October 30, 2026