iFluor® 680 amine

Name: iFluor® 680 amine
Brand: AAT Bioquest
SKU: 1076
Price: 227 USD
Availability: InStock

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, and 633 nm). iFluor® 680 dyes have fluorescence excitation and emission maxima of ~684 nm and ~701 nm respectively. These spectral characteristics make them an excellent alternative to Cy5.5® and Alexa Fluor® 680 labeling dyes (Cy5.5® and Alexa Fluor® are the trademarks of GE Health Care and Invitrogen). iFluor® 680 amine is stable and used for modifying carbonyl groups (e.g., aldehyde and carboxy groups).

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 680 amine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	97.179 µL	485.894 µL	971.789 µL	4.859 mL	9.718 mL
5 mM	19.436 µL	97.179 µL	194.358 µL	971.789 µL	1.944 mL
10 mM	9.718 µL	48.589 µL	97.179 µL	485.894 µL	971.789 µL

Molarity calculator

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Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
iFluor® 350 amine	345	450	20000¹	0.95¹	0.83	0.23
iFluor® 405 amine	403	427	37000¹	0.91¹	0.48	0.77
iFluor® 488 amine	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 555 amine	557	570	100000¹	0.64¹	0.23	0.14
iFluor® 647 amine	656	670	250000¹	0.25¹	0.03	0.03
iFluor® 660 amine	663	678	250000¹	0.26¹	0.07	0.08
iFluor® 700 amine	690	713	220000¹	0.23¹	0.09	0.04
iFluor® 710 amine	716	739	150000¹	0.60¹	0.12	0.07
iFluor® 750 amine	757	779	275000¹	0.12¹	0.044	0.039
iFluor® 790 amine	787	812	250000¹	0.13¹	0.1	0.09
iFluor® 680 Styramide Superior Replacement for Alexa Fluor 680 tyramide and Opal 690	684	701	220000¹	0.23¹	0.097	0.094
iFluor® 680 Tyramide Superior Replacement for Opal 690	684	701	220000¹	0.23¹	0.097	0.094
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Citations

View all 4 citations: Citation Explorer

MAbTope: A Method for Improved Epitope Mapping
Authors: Bourquard, Thomas and Musnier, Astrid and Puard, Vincent and Tahir, Shifa and Ayoub, Mohammed Akli and Jullian, Yann and Boulo, Thomas and Gallay, Nathalie and Watier, Hervé and Bruneau, Gilles and others, undefined
Journal: The Journal of Immunology (2018): ji1701722

Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification
Authors: Gao, D and Liu, N and Li, Y and Zhang, Y and Liu, G and others, undefined
Journal: Metabolomics (Los Angel) (2017): 2153--0769

Suramin inhibits cullin-RING E3 ubiquitin ligases
Authors: Wu, Kenneth and Chong, Robert A and Yu, Qing and Bai, Jin and Spratt, Donald E and Ching, Kevin and Lee, Chan and Miao, Haibin and Tappin, Inger and Hurwitz, Jerard and others, undefined
Journal: Proceedings of the National Academy of Sciences (2016): E2011--E2018

Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function
Authors: Piccard, Helene and Berghmans, Nele and Korpos, Eva and Dillen, Chris and Aelst, Ilse Van and Li, S and ra , undefined and Martens, Erik and Liekens, S and ra , undefined and Noppen, Sam and Damme, Jo Van and others, undefined
Journal: International Journal of Cancer (2012): E425--E436

References

View all 49 references: Citation Explorer

Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes
Authors: Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.
Journal: Mol Biosyst (2011): 1766

Visualizing dengue virus through Alexa Fluor labeling
Authors: Zhang S, Tan HC, Ooi EE.
Journal: J Vis Exp. (2011)

Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells
Authors: Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.
Journal: Biochem Biophys Res Commun (2011): 211

Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594
Authors: Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.
Journal: Zhen Ci Yan Jiu (2011): 262

Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers
Authors: Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.
Journal: J Microbiol Methods (2011): 119

Page updated on October 22, 2024

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Catalog Number	1076
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Physical properties

Molecular weight	1029.03
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.097
Correction Factor (280 nm)	0.094
Extinction coefficient (cm ^-1 M ^-1)	220000¹
Excitation (nm)	684
Emission (nm)	701
Quantum yield	0.23¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501