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AAT Bioquest

iFluor® 680 amine

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, and 633 nm). iFluor® 680 dyes have fluorescence excitation and emission maxima of ~684 nm and ~701 nm respectively. These spectral characteristics make them an excellent alternative to Cy5.5® and Alexa Fluor® 680 labeling dyes (Cy5.5® and Alexa Fluor® are the trademarks of GE Health Care and Invitrogen). iFluor® 680 amine is stable and used for modifying carbonyl groups (e.g., aldehyde and carboxy groups).

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 680 amine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM97.179 µL485.894 µL971.789 µL4.859 mL9.718 mL
5 mM19.436 µL97.179 µL194.358 µL971.789 µL1.944 mL
10 mM9.718 µL48.589 µL97.179 µL485.894 µL971.789 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
iFluor® 350 amine3454502000010.9510.830.23
iFluor® 405 amine4034273700010.9110.480.77
iFluor® 488 amine4915167500010.910.210.11
iFluor® 555 amine55757010000010.6410.230.14
iFluor® 647 amine65667025000010.2510.030.03
iFluor® 660 amine66367825000010.2610.070.08
iFluor® 700 amine69071322000010.2310.090.04
iFluor® 710 amine71673915000010.6010.120.07
iFluor® 750 amine75777927500010.1210.0440.039
iFluor® 790 amine78781225000010.1310.10.09
iFluor® 680 Styramide *Superior Replacement for Alexa Fluor 680 tyramide and Opal 690*68470122000010.2310.0970.094
iFluor® 680 Tyramide *Superior Replacement for Opal 690*68470122000010.2310.0970.094
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Citations

View all 4 citations: Citation Explorer
MAbTope: A Method for Improved Epitope Mapping
Authors: Bourquard, Thomas and Musnier, Astrid and Puard, Vincent and Tahir, Shifa and Ayoub, Mohammed Akli and Jullian, Yann and Boulo, Thomas and Gallay, Nathalie and Watier, Hervé and Bruneau, Gilles and others, undefined
Journal: The Journal of Immunology (2018): ji1701722
Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification
Authors: Gao, D and Liu, N and Li, Y and Zhang, Y and Liu, G and others, undefined
Journal: Metabolomics (Los Angel) (2017): 2153--0769
Suramin inhibits cullin-RING E3 ubiquitin ligases
Authors: Wu, Kenneth and Chong, Robert A and Yu, Qing and Bai, Jin and Spratt, Donald E and Ching, Kevin and Lee, Chan and Miao, Haibin and Tappin, Inger and Hurwitz, Jerard and others, undefined
Journal: Proceedings of the National Academy of Sciences (2016): E2011--E2018
Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function
Authors: Piccard, Helene and Berghmans, Nele and Korpos, Eva and Dillen, Chris and Aelst, Ilse Van and Li, S and ra , undefined and Martens, Erik and Liekens, S and ra , undefined and Noppen, Sam and Damme, Jo Van and others, undefined
Journal: International Journal of Cancer (2012): E425--E436

References

View all 49 references: Citation Explorer
Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes
Authors: Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.
Journal: Mol Biosyst (2011): 1766
Visualizing dengue virus through Alexa Fluor labeling
Authors: Zhang S, Tan HC, Ooi EE.
Journal: J Vis Exp. (2011)
Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells
Authors: Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.
Journal: Biochem Biophys Res Commun (2011): 211
Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594
Authors: Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.
Journal: Zhen Ci Yan Jiu (2011): 262
Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers
Authors: Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.
Journal: J Microbiol Methods (2011): 119
Page updated on November 1, 2024

Ordering information

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Catalog Number1076
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Physical properties

Molecular weight

1029.03

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.097

Correction Factor (280 nm)

0.094

Extinction coefficient (cm -1 M -1)

2200001

Excitation (nm)

684

Emission (nm)

701

Quantum yield

0.231

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Fluorescent dye amines are the most popular tool for conjugating dyes to a target compound with a carbonyl group (e.g., aldehyde, carboxylic acid or activated carboxy group such as NHS ester).
Fluorescent dye amines are the most popular tool for conjugating dyes to a target compound with a carbonyl group (e.g., aldehyde, carboxylic acid or activated carboxy group such as NHS ester).
Fluorescent dye amines are the most popular tool for conjugating dyes to a target compound with a carbonyl group (e.g., aldehyde, carboxylic acid or activated carboxy group such as NHS ester).