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iFluor® Ultra 647 maleimide
The iFluor® Ultra series represents an enhancement of our established iFluor® dyes, optimized for antibody labeling in fluorescence imaging and flow cytometry. Within this series, iFluor® Ultra 647 is an exceptionally bright far-red fluorescent dye excitable by 594 nm or 633 nm laser lines, with a peak emission at 670 nm. Antibody conjugates with iFluor® Ultra 647 exhibit superior brightness compared to those labeled with Alexa Fluor® 647 under identical conditions. Additionally, iFluor® Ultra 647 maintains stable fluorescence across a pH range of 4 to 10. The maleimide derivative of iFluor® Ultra 647 is widely used for conjugation to thiol groups on proteins, oligonucleotide thiophosphates, or low molecular weight ligands. Conjugates with iFluor® Ultra 647 demonstrate higher fluorescence intensity and greater photostability compared to those with other spectrally similar fluorophores, enhancing their utility for advanced fluorescence applications. Fluorescent dye-conjugated antibodies are crucial for protein identification in various applications, including fluorescent cell imaging, flow cytometry, western blotting, and immunohistochemistry. The advantages of these conjugates include increased sensitivity, multiplexing capabilities, and ease of use, facilitating complex biological studies.
Fluorescent dye maleimides are the most popular tool for conjugating dyes to a peptide, protein, antibody, thiol-modified oligonucleotide or nucleic acid through their SH group. Maleimides react readily with the thiol group of proteins, thiol-modified oligonucleotides, and other thiol-containing molecules under neutral conditions. The resulting dye conjugates are quite stable.
Fluorescent dye maleimides are the most popular tool for conjugating dyes to a peptide, protein, antibody, thiol-modified oligonucleotide or nucleic acid through their SH group. Maleimides react readily with the thiol group of proteins, thiol-modified oligonucleotides, and other thiol-containing molecules under neutral conditions. The resulting dye conjugates are quite stable.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
716731 mg
Price
 
Physical properties
Molecular weight2672.16
SolventDMSO
Spectral properties
Absorbance (nm)654
Correction factor (260 nm)0.07
Correction factor (280 nm)0.07
Extinction coefficient (cm -1 M -1)
250000
1
Excitation (nm)655
Emission (nm)670
Quantum yield
0.39
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on October 23, 2025