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iFluor® 488 PSA™ Imaging Kit with Goat Anti-Rabbit IgG
Power Styramide™ Signal Amplification (PSA™) system is one of the most sensitive methods that can detect extremely low-abundance targets in cells and tissues with improved fluorescence signal 10-50 times higher than the widely used tyramide (TSA) reagents. In combination with our superior iFluor® dyes that have higher florescence intensity, increased photostability and enhanced water solubility, the iFluor® dye-labeled Styramide™ conjugates can generate fluorescence signal with significantly higher precision and sensitivity (more than 100 times) than standard ICC/IF/IHC. PSA™ utilizes the catalytic activity of horseradish peroxidase (HRP) for covalent deposition of fluorophores in situ. PSA™ radicals have much higher reactivity than tyramide radicals, making the PSA™ system much faster, more robust and sensitive than the traditional TSA reagents. Compared to tyramide reagents, the Styramide™ conjugates have ability to label the target at higher efficiency and thus generate significantly higher fluorescence signal. Styramide™ conjugates also allow significantly less consumption of primary antibody compared to standard directly conjugate method or tyramide amplification with the same level of sensitivity. iFluor® 488 PSA™ kit is a much superior replacement for Alexa Fluor 488 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.
Fluorescence IHC of formaldehyde-fixed, paraffin-embedded human lung adenocarcinoma using two imaging signal amplification kits. Tissue sections were stained with rabbit anti-EpCam antibody and then followed PSA™ method using iFluor® 488 PSA™ Imaging Kit with goat anti-rabbit IgG (Cat#45205) or Thermo Fisher Alexa Fluor® 488 Superboost Kit  respectively. Cell nucleus were stained with Nuclear Blue™ DCS1 (Cat#17548).
Fluorescence IHC of formaldehyde-fixed, paraffin-embedded human lung adenocarcinoma using two imaging signal amplification kits. Tissue sections were stained with rabbit anti-EpCam antibody and then followed PSA™ method using iFluor® 488 PSA™ Imaging Kit with goat anti-rabbit IgG (Cat#45205) or Thermo Fisher Alexa Fluor® 488 Superboost Kit  respectively. Cell nucleus were stained with Nuclear Blue™ DCS1 (Cat#17548).
protocol
Protocol
sds
SDS
CatalogSize
Price
Quantity
45205100 Tests
Price
 
Spectral properties
Correction factor (260 nm)0.21
Correction factor (280 nm)0.11
Extinction coefficient (cm -1 M -1)
75000
1
Excitation (nm)491
Emission (nm)516
Quantum yield
0.9
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microscope
ExcitationFITC filter set
EmissionFITC filter set
Recommended plateBlack wall/clear bottom
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Power Styramide™ Signal Amplification (PSA™)
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Page updated on December 18, 2025