FluoroQuest™ TSA/PSA Antifade Mounting Medium *Optimized for Tyramide and Styramide Imaging*
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Refrigerated (2-8 °C) |
Related products
Overview | ![]() ![]() |
See also: Immunofluorescence, Mounting Media for Microscopy
FluoroQuest™ TSA/PSA Antifade Mounting Medium is the only mouitng medium that is optimized for tyramide (TSA) and styramide (PSA)-based fluorescence imaging applications. The antifade mounting medium is a specialized solution used in fluorescence microscopy to preserve the fluorescent signal emitted by fluorophores of TSA and PSA probes. When using fluorescent probes to label cellular components, it's crucial to preserve the fluorescence and prevent fading during the imaging process. Fluorescence fading occurs due to photobleaching, where the fluorophores lose their ability to emit light when exposed to intense repeated excitation light. The antifade mounting medium is designed to minimize photobleaching and maintain the fluorescence of the labeled structures over an extended period. This medium contains a combination of a few components that help protect the fluorophores and stabilize the fluorescent signal, including antioxidants, antifading agents, buffering agents and viscosity modifiers. AAT Bioquest also offers a few other mounting media for the general fluorescence imaging applications. It's essential to choose the appropriate antifade mounting medium based on the specific experimental needs and the fluorophores used. After mounting the sample with antifade medium, it is typically covered with a glass coverslip to protect it and then imaged using fluorescence microscopy to visualize and analyze the labeled structures.
Images
References
View all 2 references: Citation Explorer
Direct immunofluorescent labeling of cells.
Authors: Pástor, Maria Veronica Dávila
Journal: Methods in molecular biology (Clifton, N.J.) (2010): 135-42
Authors: Pástor, Maria Veronica Dávila
Journal: Methods in molecular biology (Clifton, N.J.) (2010): 135-42
Quantitative comparison of anti-fading mounting media for confocal laser scanning microscopy.
Authors: Ono, M and Murakami, T and Kudo, A and Isshiki, M and Sawada, H and Segawa, A
Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (2001): 305-12
Authors: Ono, M and Murakami, T and Kudo, A and Isshiki, M and Sawada, H and Segawa, A
Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (2001): 305-12
Application notes
A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
FAQ
Are Cell Navigator® Cell Plasma Membrane Staining Kits suitable for cell culture medium samples?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?
Are there any calcium indicators that don't require probenecid (PBC)?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?
Are there any calcium indicators that don't require probenecid (PBC)?