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FluoroQuest™ Anti-fading Kit II
Optimized for Plate Imaging
When exposed to excitation light, fluorescence intensity of dyes decreases due to their photooxidation or other photoreactions. There are very few fluorescent dyes that completely resist photobleaching. Frequently, when a section has been scanned repeatedly under strong excitation light, dyes could lose significant fluorescence signal before visual evaluation or photography can be accomplished. For examples, the photobleaching of fluoresceins (such as FITC-labeled antibodies) has become a major problem in fluorescence microscopy. In severe cases (such as phycoprotein-labeled bioconjugates), a fluorescence image of high resolution can not even be taken due to the extremely high photobleaching rate. Fluoroquest™ Anti-Fading Kit is to reduce the dye photobleaching rate, giving researchers longer observation time. The kit contains all the essential components that can be readily applied to imaging experiments. They are all premixed and ready-to-use solutions. This kit is designed for microplate format while #20001 is designed for slide format.
U2OS cells were loaded with 1 µM Calcein AM for 1 hour, fixing with 2% formaldehyde for 30 minutes in a 96-well Costar black plate. Anti-fading reagent (100 µL/well) was added into the samples after removing all the media. The FITC signals were compared at 0 and 30 seconds exposure time by using Olympus fluorescence microscopy. The same exposure settings were used for all the images.
U2OS cells were loaded with 1 µM Calcein AM for 1 hour, fixing with 2% formaldehyde for 30 minutes in a 96-well Costar black plate. Anti-fading reagent (100 µL/well) was added into the samples after removing all the media. The FITC signals were compared at 0 and 30 seconds exposure time by using Olympus fluorescence microscopy. The same exposure settings were used for all the images.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
200031 kit
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microscope
ExcitationVaries
EmissionVaries
Recommended plateBlack wall/clear bottom
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on October 26, 2025