FluoroQuest™ TSA/PSA Antifade Mounting Medium *Optimized for Tyramide and Styramide Imaging*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Refrigerated (2-8 °C); Minimize light exposure |
Related products
Overview | SDSProtocol |
See also: Immunofluorescence, Mounting Media for Microscopy
FluoroQuest™ TSA/PSA Antifade Mounting Medium is the only mouitng medium that is optimized for tyramide (TSA) and styramide (PSA)-based fluorescence imaging applications. The antifade mounting medium is a specialized solution used in fluorescence microscopy to preserve the fluorescent signal emitted by fluorophores of TSA and PSA probes. When using fluorescent probes to label cellular components, it's crucial to preserve the fluorescence and prevent fading during the imaging process. Fluorescence fading occurs due to photobleaching, where the fluorophores lose their ability to emit light when exposed to intense repeated excitation light. The antifade mounting medium is designed to minimize photobleaching and maintain the fluorescence of the labeled structures over an extended period. This medium contains a combination of a few components that help protect the fluorophores and stabilize the fluorescent signal, including antioxidants, antifading agents, buffering agents and viscosity modifiers. AAT Bioquest also offers a few other mounting media for the general fluorescence imaging applications. It's essential to choose the appropriate antifade mounting medium based on the specific experimental needs and the fluorophores used. After mounting the sample with antifade medium, it is typically covered with a glass coverslip to protect it and then imaged using fluorescence microscopy to visualize and analyze the labeled structures.
Images
References
View all 2 references: Citation Explorer
Direct immunofluorescent labeling of cells.
Authors: Pástor, Maria Veronica Dávila
Journal: Methods in molecular biology (Clifton, N.J.) (2010): 135-42
Authors: Pástor, Maria Veronica Dávila
Journal: Methods in molecular biology (Clifton, N.J.) (2010): 135-42
Quantitative comparison of anti-fading mounting media for confocal laser scanning microscopy.
Authors: Ono, M and Murakami, T and Kudo, A and Isshiki, M and Sawada, H and Segawa, A
Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (2001): 305-12
Authors: Ono, M and Murakami, T and Kudo, A and Isshiki, M and Sawada, H and Segawa, A
Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (2001): 305-12
Application notes
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FAQ
How can I lyse my cells without lysing the nuclear membrane?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?