FluoroQuest™ Anti-fading Mounting Medium with DAPI

FluoroQuest™ with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This mounting medium contains anti-fading agents to reduce the dye photobleaching rate, and is fortified with DAPI which is a counterstain for DNA. For use in in situ hybridization techniques or other methods where fluorescence of DNA staining is required. DAPI excites at 360 nm and emits at 460 nm, producing a blue fluorescence. RNA is also stained with DAPI.

<strong>Fluorescence IHC of formaldehyde-fixed, </strong><strong>paraffin-embedded Human </strong><strong>lung adenocarcinoma positive </strong><strong>tissue. </strong>Human lung adenocarcinoma positive tissue sections were stained with rabbit anti-EpCam antibody and then incubated with polyHRP-labeled Goat anti-Rabbit IgG secondary antibody followed by iFluor® 488 Styramide™ (Cat#45020) stain respectively. The tissue was mounted in FluoroQuest™ Anti-fading Mounting Medium with DAPI (cat#20005).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
20005	20 mL	Price

References

View all 26 references: Citation Explorer

Photobleaching of organic fluorophores: quantitative characterization, mechanisms, protection.

Authors:

Demchenko, Alexander P

Journal:

Methods and applications in fluorescence (2020): 022001

The Relationship between Residual Amount of Sr and Morphology of Eutectic Si Phase in A356 Alloy.

Authors:

Zhang, Wenda and Ma, Shixuan and Wei, Zhenhua and Bai, Peikang

Journal:

Materials (Basel, Switzerland) (2019)

[Multi-color immunofluorescence microscopy in cultured cells].

Authors:

Matsuzaki, Toshiyuki

Journal:

Nihon yakurigaku zasshi. Folia pharmacologica Japonica (2019): 165-170

Using Phospho-Peptides Immobilized on Magnetic Beads for Absorption Control in Immunohistochemistry.

Authors:

Schwartz, David and Grahek, Michael and He, Yingwei and Wang, Wei and Nguyen, Jennifer and Kalyuzhny, Alexander E

Journal:

Methods in molecular biology (Clifton, N.J.) (2017): 219-227

FAD-I, a Fusobacterium nucleatum Cell Wall-Associated Diacylated Lipoprotein That Mediates Human Beta Defensin 2 Induction through Toll-Like Receptor-1/2 (TLR-1/2) and TLR-2/6.

Authors:

Bhattacharyya, Sanghamitra and Ghosh, Santosh K and Shokeen, Bhumika and Eapan, Betty and Lux, Renate and Kiselar, Janna and Nithianantham, Stanley and Young, Andrew and Pandiyan, Pushpa and McCormick, Thomas S and Weinberg, Aaron

Journal:

Infection and immunity (2016): 1446-1456

Physical properties

Solvent

Water

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	27000
Excitation (nm)	359
Emission (nm)	457

Storage, safety and handling

H-phrase	H303, H313, H340
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R68
Storage	Refrigerated (2-8 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on September 26, 2025