FluoroQuest™ Anti-fading Kit I *Optimized for Slide Imaging*

FluoroQuest™ Anti-fading Kit I

Optimized for Slide Imaging

When exposed to excitation light, fluorescence intensity of dyes decreases due to their photooxidation or other photoreactions. There are very few fluorescent dyes that completely resist photobleaching. Frequently, when a section has been scanned repeatedly under strong excitation light, dyes could lose significant fluorescence signal before visual evaluation or photography can be accomplished. For examples, the photobleaching of fluoresceins (such as FITC-labeled antibodies) has become a major problem in fluorescence microscopy. In severe cases (such as phycoprotein-labeled bioconjugates), a fluorescence image of high resolution can not even be taken due to the extremely high photobleaching rate. Fluoroquest™ Anti-Fading Kit is to reduce the dye photobleaching rate, giving researchers longer observation time. The kit contains all the essential components that can be readily applied to imaging experiments. They are all premixed and ready-to-use solutions. This kit is designed for slide format while #20003 is designed for microplate format.

U2OS cells in a 96-well Costar black plate were loaded with 1 µM calcein, AM for 1 hour, fixing with 2% formaldehyde for 30 minutes. Anti-fading reagents were added to the samples after removing all the media. The FITC signals were compared at 0 and 30 seconds exposure time by using an Olympus fluorescence microscopy. The same exposure settings were used for all the images.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
20001	1 kit	Price

References

View all 18 references: Citation Explorer

On the mechanism of Trolox as antiblinking and antibleaching reagent

Authors:

Cordes T, Vogelsang J, Tinnefeld P.

Journal:

J Am Chem Soc (2009): 5018

Relationship between DAPI-fluorescence fading and nuclear DNA content: An alternative method to DNA quantification

Authors:

Gallardo-Escarate C, Alvarez-Borrego J, Von Br and E, Dupre E, Del Rio-Portilla MA.

Journal:

Biol Res (2007): 29

Strategies to improve photostabilities in ultrasensitive fluorescence spectroscopy

Authors:

Widengren J, Chmyrov A, Eggeling C, Lofdahl PA, Seidel CA.

Journal:

J Phys Chem A (2007): 429

The reliability of long-term storage of direct immunofluorescent staining slides at room temperature

Authors:

Dikicioglu E, Meteoglu I, Okyay P, Culhaci N, Kacar F.

Journal:

J Cutan Pathol (2003): 430

Comparative genomic hybridization technique

Authors:

El-Rifai WE, Knuutila S.

Journal:

Methods Mol Med (2001): 25

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Fluorescence microscope
Excitation	Varies
Emission	Varies
Recommended plate	Black wall/clear bottom

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Page updated on October 13, 2025

Component A: AFRG515	1 vial (1 mL)
Component B: AFRM520	1 vial (1 mL)
Component C: AFRR560	1 vial (1 mL)