FluoroQuest™ Fluorescence Signal Enhancing Solution

HeLa cells were incubated with (Tubulin+, Upper) or without (Tubulin-, Bottom) mouse tubulin antibody for 30 minutes at room temperature. After 3 times wash in PBS, cells were stained using iFluor® 488 goat anti-mouse IgG conjugate (Cat#16528) diluted without (Left) or with FluoroQuest™ fluorescence signal enhancing solution (Right), respectively.

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

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Storage, safety and handling

H-phrase	H303, H313, H340
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R68
Storage	Freeze (< -15 °C)
UNSPSC	12352200

Overview SDS Protocol

When proteins are conjugated to fluorescent organic dyes, fluorescence emission of the dye molecules is usually decreased, sometimes up to 50-70%. This quenching phenomenon has been acknowledged for decades, but as yet, there are no simple, practical methods to control the fluorescence of dyes conjugated to proteins, especially for dyes conjugated to immunoglobulins. We offer this FluoroQuest™ fluorescence signal enhancing solution that might increase fluorescence up to 2.5-fold in cell imaging and flow analysis. This ready-to-use solution provides an effective way to increase the sensitivity of detection of fluorescent organic labels used in immunology, histochemistry, and cell biology.

Platform

Fluorescence microscope

Recommended plate

Black wall/clear bottom

Images

Figure 1. HeLa cells were incubated with (Tubulin+, Upper) or without (Tubulin-, Bottom) mouse tubulin antibody for 30 minutes at room temperature. After 3 times wash in PBS, cells were stained using iFluor® 488 goat anti-mouse IgG conjugate (Cat#16528) diluted without (Left) or with FluoroQuest™ fluorescence signal enhancing solution (Right), respectively.

References

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