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FluoroQuest™ Fluorescence Signal Enhancing Solution

When proteins are conjugated to fluorescent organic dyes, fluorescence emission of the dye molecules is usually decreased, sometimes up to 50-70%. This quenching phenomenon has been acknowledged for decades, but as yet, there are no simple, practical methods to control the fluorescence of dyes conjugated to proteins, especially for dyes conjugated to immunoglobulins. We offer this FluoroQuest™ fluorescence signal enhancing solution that might increase fluorescence up to 2.5-fold in cell imaging and flow analysis. This ready-to-use solution provides an effective way to increase the sensitivity of detection of fluorescent organic labels used in immunology, histochemistry, and cell biology.

References

View all 18 references: Citation Explorer
On the mechanism of Trolox as antiblinking and antibleaching reagent
Authors: Cordes T, Vogelsang J, Tinnefeld P.
Journal: J Am Chem Soc (2009): 5018
Relationship between DAPI-fluorescence fading and nuclear DNA content: An alternative method to DNA quantification
Authors: Gallardo-Escarate C, Alvarez-Borrego J, Von Br and E, Dupre E, Del Rio-Portilla MA.
Journal: Biol Res (2007): 29
Strategies to improve photostabilities in ultrasensitive fluorescence spectroscopy
Authors: Widengren J, Chmyrov A, Eggeling C, Lofdahl PA, Seidel CA.
Journal: J Phys Chem A (2007): 429
The reliability of long-term storage of direct immunofluorescent staining slides at room temperature
Authors: Dikicioglu E, Meteoglu I, Okyay P, Culhaci N, Kacar F.
Journal: J Cutan Pathol (2003): 430
Comparative genomic hybridization technique
Authors: El-Rifai WE, Knuutila S.
Journal: Methods Mol Med (2001): 25
Page updated on December 1, 2024

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Catalog Number20006
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Additional ordering information

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Storage, safety and handling

H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68

Storage

Freeze (< -15 °C)
UNSPSC12352200

Platform

Fluorescence microscope

Recommended plateBlack wall, clear bottom
HeLa cells were incubated with (Tubulin+, Upper) or without (Tubulin-, Bottom) mouse tubulin antibody for 30 minutes at room temperature. After 3 times wash in PBS, cells were stained using iFluor®&nbsp;488 goat anti-mouse IgG conjugate (Cat#16528) diluted without (Left) or with FluoroQuest&trade; fluorescence signal enhancing solution (Right), respectively.
HeLa cells were incubated with (Tubulin+, Upper) or without (Tubulin-, Bottom) mouse tubulin antibody for 30 minutes at room temperature. After 3 times wash in PBS, cells were stained using iFluor®&nbsp;488 goat anti-mouse IgG conjugate (Cat#16528) diluted without (Left) or with FluoroQuest&trade; fluorescence signal enhancing solution (Right), respectively.
HeLa cells were incubated with (Tubulin+, Upper) or without (Tubulin-, Bottom) mouse tubulin antibody for 30 minutes at room temperature. After 3 times wash in PBS, cells were stained using iFluor®&nbsp;488 goat anti-mouse IgG conjugate (Cat#16528) diluted without (Left) or with FluoroQuest&trade; fluorescence signal enhancing solution (Right), respectively.