FluoroQuest™ Fluorescence Signal Enhancing Solution

When proteins are conjugated to fluorescent organic dyes, fluorescence emission of the dye molecules is usually decreased, sometimes up to 50-70%. This quenching phenomenon has been acknowledged for decades, but as yet, there are no simple, practical methods to control the fluorescence of dyes conjugated to proteins, especially for dyes conjugated to immunoglobulins. We offer this FluoroQuest™ fluorescence signal enhancing solution that might increase fluorescence up to 2.5-fold in cell imaging and flow analysis. This ready-to-use solution provides an effective way to increase the sensitivity of detection of fluorescent organic labels used in immunology, histochemistry, and cell biology.

<p>HeLa cells were incubated with (Tubulin+, Upper) or without (Tubulin-, Bottom) mouse tubulin antibody for 30 minutes at room temperature. After 3 times wash in PBS, cells were stained using iFluor® 488 goat anti-mouse IgG conjugate (Cat#16528) diluted without (Left) or with FluoroQuest™ fluorescence signal enhancing solution (Right), respectively.</p>

Protocol

SDS

COA

Catalog	Size	Price	Quantity
20006	5 mL	Price

References

View all 18 references: Citation Explorer

On the mechanism of Trolox as antiblinking and antibleaching reagent

Authors:

Cordes T, Vogelsang J, Tinnefeld P.

Journal:

J Am Chem Soc (2009): 5018

Relationship between DAPI-fluorescence fading and nuclear DNA content: An alternative method to DNA quantification

Authors:

Gallardo-Escarate C, Alvarez-Borrego J, Von Br and E, Dupre E, Del Rio-Portilla MA.

Journal:

Biol Res (2007): 29

Strategies to improve photostabilities in ultrasensitive fluorescence spectroscopy

Authors:

Widengren J, Chmyrov A, Eggeling C, Lofdahl PA, Seidel CA.

Journal:

J Phys Chem A (2007): 429

The reliability of long-term storage of direct immunofluorescent staining slides at room temperature

Authors:

Dikicioglu E, Meteoglu I, Okyay P, Culhaci N, Kacar F.

Journal:

J Cutan Pathol (2003): 430

Comparative genomic hybridization technique

Authors:

El-Rifai WE, Knuutila S.

Journal:

Methods Mol Med (2001): 25

Storage, safety and handling

H-phrase	H303, H313, H340
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R68
Storage	Freeze (< -15 °C)
UNSPSC	12352200

Instrument settings

Fluorescence microscope
Recommended plate	Black wall/clear bottom

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Page updated on October 13, 2025