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FluoroQuest™ Fluorescence Signal Enhancing Solution

HeLa cells were incubated with (Tubulin+, Upper) or without (Tubulin-, Bottom) mouse tubulin antibody for 30 minutes at room temperature. After 3 times wash in PBS, cells were stained using iFluor® 488 goat anti-mouse IgG conjugate (Cat#16528) diluted without (Left) or with FluoroQuest™ fluorescence signal enhancing solution (Right), respectively.
HeLa cells were incubated with (Tubulin+, Upper) or without (Tubulin-, Bottom) mouse tubulin antibody for 30 minutes at room temperature. After 3 times wash in PBS, cells were stained using iFluor® 488 goat anti-mouse IgG conjugate (Cat#16528) diluted without (Left) or with FluoroQuest™ fluorescence signal enhancing solution (Right), respectively.
HeLa cells were incubated with (Tubulin+, Upper) or without (Tubulin-, Bottom) mouse tubulin antibody for 30 minutes at room temperature. After 3 times wash in PBS, cells were stained using iFluor® 488 goat anti-mouse IgG conjugate (Cat#16528) diluted without (Left) or with FluoroQuest™ fluorescence signal enhancing solution (Right), respectively.
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Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Storage, safety and handling
H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
StorageFreeze (< -15 °C)
UNSPSC12352200

OverviewpdfSDSpdfProtocol


When proteins are conjugated to fluorescent organic dyes, fluorescence emission of the dye molecules is usually decreased, sometimes up to 50-70%. This quenching phenomenon has been acknowledged for decades, but as yet, there are no simple, practical methods to control the fluorescence of dyes conjugated to proteins, especially for dyes conjugated to immunoglobulins. We offer this FluoroQuest™ fluorescence signal enhancing solution that might increase fluorescence up to 2.5-fold in cell imaging and flow analysis. This ready-to-use solution provides an effective way to increase the sensitivity of detection of fluorescent organic labels used in immunology, histochemistry, and cell biology.

Platform


Fluorescence microscope

Recommended plateBlack wall/clear bottom

Images


References


View all 18 references: Citation Explorer
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