10X Citrate Buffer *pH 6.0*
|Shipping||Standard overnight for United States, inquire for international|
|H-phrase||H303, H313, H333|
|Intended use||Research Use Only (RUO)|
|R-phrase||R20, R21, R22|
|Storage||Refrigerated (2-8 °C)|
Heat-Induced Epitope (Antigen) Retrieval (HIER) techniques are a common application that uses a citrate buffer. These techniques help regain lost antigenicity that may occur in formalin-fixed paraffin-embedded (FFPE) tissue. During fixation, formalin and other aldehyde compounds are hypothesized to create protein crosslinks with antigenic sites in tissue specimens, resulting in conformational changes that mask these epitopes leading to poor downstream detection during fluorescence in situ hybridization (FISH) or immunohistochemistry (IHC). Applying HIER, which uses heat treatment in citrate buffer (0.01 - 0.1 M), aldehyde crosslinks formed during fixation are theorized to be reduced, restoring lost antigenicity and improving downstream detection and signal quality. (Krenacs 2010).
- Dawson, Rex Malcolm Chaplin, et al. Data for biochemical research. Vol. 3. Clarendon press, 2002.
- Krenacs, Laszlo, et al. "Heat-induced antigen retrieval for immunohistochemical reactions in routinely processed paraffin sections." Immunocytochemical Methods and Protocols. Humana Press, 2010. 103-119.
- Nozawa, S. R., et al. "Mind the buffering capacity of citric acid." Fungal Genetics Newsletter 42 (1995): 56.
AT A GLANCE
2-8°C. DO NOT FREEZE
1X buffer solution is intended for heat-induced antigen retrieval in IHC. Please refer to the primary antibody protocol.
10X, pH 6 Citrate Buffer.
PREPARATION OF WORKING SOLUTION
Dilute 10X buffer as needed (e.g., 10 mL of 10X Citrate buffer + 90 mL of distilled water), and mix well.
Note: 1X citrate buffer can be stored at 2-8°C.