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ReadiUse™ microscope mounting solution

Fluorescence image of HeLa cells fixed with 4% formaldehyde (Cat#20010) then stained with Cell Navigator® F-Actin Labeling Kit (Cat#22661).<br />A)Image of Hela cells without adding mounting solution<br />B)Image of Hela cells after adding mounting medium (Cat#20009).<br />C) Image of Hela cells after adding mounting medium for 24 hours.
Fluorescence image of HeLa cells fixed with 4% formaldehyde (Cat#20010) then stained with Cell Navigator® F-Actin Labeling Kit (Cat#22661).<br />A)Image of Hela cells without adding mounting solution<br />B)Image of Hela cells after adding mounting medium (Cat#20009).<br />C) Image of Hela cells after adding mounting medium for 24 hours.
Fluorescence image of HeLa cells fixed with 4% formaldehyde (Cat#20010) then stained with Cell Navigator® F-Actin Labeling Kit (Cat#22661).<br />A)Image of Hela cells without adding mounting solution<br />B)Image of Hela cells after adding mounting medium (Cat#20009).<br />C) Image of Hela cells after adding mounting medium for 24 hours.
Protective effect of RC on Aβ<sub>25–35</sub>-induced cytotoxicity. After mounting on glass slides using ReadiUse™ microscope mounting solution, the coverslips were observed using a LSM 510 confocal laser scanning microscope. Cells with the above treatments were fixed and visualized by immunofluorescence microscope using anti-β-III tubulin antibody (green channel) costained with antitubulinΔCsp6 antibody that specifically recognized caspase-6-cleaved tubulin (red channel). Nuclei were revealed by staining with DAPI. Merged images show the superimposition of both green and red signals, with overlapping areas appearing yellow. Aβ25–35 treatment induced caspase-mediated cleavage of tubulin, indicative of apoptosis. Source: <b>Rhinacanthin C Alleviates Amyloid-β Fibrils' Toxicity on Neurons and Attenuates Neuroinflammation Triggered by LPS, Amyloid-β, and Interferon-γ in Glial Cells</b> by Chuang, Kai-An et.al., <em>Oxidative Medicine and Cellular Longevity</em>, Oct. 2017.
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Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure
UNSPSC12352200
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OverviewpdfSDSpdfProtocol


The mounting medium is the solution in which the specimen is embedded, generally under a cover glass. Simple liquids like water or glycerol can be considered mounting media. Properties of a good mounting medium include non-reactivity with the specimen, stability over time without crystallizing, darkening, or changing refractive index, solubility in the medium the specimen was prepared in, and not causing the specimen stain to fade or leach. All our ReadiUse™ reagents require minimal hands-on time. This microscope mounting solution is a ready-to-apply format.

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Citations


View all 6 citations: Citation Explorer
High-frequency stimulation of the subthalamic nucleus activates motor cortex pyramidal tract neurons by a process involving local glutamate, GABA and dopamine receptors in hemi-parkinsonian rats
Authors: Chuang, Chi-Fen and Wu, Chen-Wei and Weng, Ying and Hu, Pei-San and Yeh, Shin-Rung and Chang, Yen-Chung
Journal: Chin. J. Physiol. (2018): 92--105
Rhinacanthin C alleviates amyloid-$\beta$ fibrils’ toxicity on neurons and attenuates neuroinflammation triggered by LPS, amyloid-$\beta$, and interferon-$\gamma$ in glial cells
Authors: Chuang, Kai-An and Li, Ming-Han and Lin, Ni-Hsuan and Chang, Chih-Hsuan and Lu, I and Pan, I and Takahashi, Tomoya and Perng, Ming-Der and Wen, Shu-Fang and others,
Journal: Oxidative Medicine and Cellular Longevity (2017)
Rhinacanthin C Alleviates Amyloid-&beta; Fibrils' Toxicity on Neurons and Attenuates Neuroinflammation Triggered by LPS, Amyloid-&beta;, and Interferon-&gamma; in Glial Cells
Authors: Chuang, Kai-An and Li, Ming-Han and Lin, Ni-Hsuan and Chang, Chih-Hsuan and Lu, I and Pan, I and Takahashi, Tomoya and Perng, Ming-Der and Wen, Shu-Fang and others, undefined
Journal: Oxidative Medicine and Cellular Longevity (2017)
Impact of siRNA targeting of &beta;-catenin on differentiation of rat neural stem cells and gene expression of Ngn1 and BMP4 following in vitro hypoxic-ischemic brain damage
Authors: Zhang, Xiaoying and Zhu, Cuicui and Luo, Qiong and Dong, Jv and Liu, Lv and Li, Min and Zhu, Hongtao and Ma, Xiangping and Wang, Jun
Journal: Molecular Medicine Reports (2016): 3595--3601
Application of interleukin-22 mediates protection in experimental acetaminophen-induced acute liver injury
Authors: Scheiermann, Patrick and Bachmann, Malte and Goren, Itamar and Zwissler, Bernhard and Pfeilschifter, Josef and M&uuml;hl, Heiko
Journal: The American journal of pathology (2013): 1107--1113
Synergistic activity of &alpha;v&beta;3 integrins and the elastin binding protein enhance cell-matrix interactions on bioactive hydrogel surfaces
Authors: Patel, Dhaval and V, undefined and romme, Susan E and Reid, Michael E and Taite, Lakeshia J
Journal: Biomacromolecules (2012): 1420--1428

References


View all 122 references: Citation Explorer
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Rational and precise design of novel fluorescence probes and their application to cellular response and in-vivo tumor imaging
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Live-cell fluorescence imaging of meiotic chromosome dynamics in Schizosaccharomyces pombe
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