ReadiUse™ microscope mounting solution

Fluorescence image of HeLa cells fixed with 4% formaldehyde (Cat#20010) then stained with Cell Navigator® F-Actin Labeling Kit (Cat#22661).<br />A)Image of Hela cells without adding mounting solution<br />B)Image of Hela cells after adding mounting medium (Cat#20009).<br />C) Image of Hela cells after adding mounting medium for 24 hours.

Protective effect of RC on Aβ<sub>25–35</sub>-induced cytotoxicity. After mounting on glass slides using ReadiUse™ microscope mounting solution, the coverslips were observed using a LSM 510 confocal laser scanning microscope. Cells with the above treatments were fixed and visualized by immunofluorescence microscope using anti-β-III tubulin antibody (green channel) costained with antitubulinΔCsp6 antibody that specifically recognized caspase-6-cleaved tubulin (red channel). Nuclei were revealed by staining with DAPI. Merged images show the superimposition of both green and red signals, with overlapping areas appearing yellow. Aβ25–35 treatment induced caspase-mediated cleavage of tubulin, indicative of apoptosis. Source: <b>Rhinacanthin C Alleviates Amyloid-β Fibrils' Toxicity on Neurons and Attenuates Neuroinflammation Triggered by LPS, Amyloid-β, and Interferon-γ in Glial Cells</b> by Chuang, Kai-An et.al., <em>Oxidative Medicine and Cellular Longevity</em>, Oct. 2017.

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H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Refrigerated (2-8 °C); Minimize light exposure
UNSPSC	12352200

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Images

Figure 1. Fluorescence image of HeLa cells fixed with 4% formaldehyde (Cat#20010) then stained with Cell Navigator® F-Actin Labeling Kit (Cat#22661).
A)Image of Hela cells without adding mounting solution
B)Image of Hela cells after adding mounting medium (Cat#20009).
C) Image of Hela cells after adding mounting medium for 24 hours.

Figure 2. Protective effect of RC on Aβ_25–35-induced cytotoxicity. After mounting on glass slides using ReadiUse™ microscope mounting solution, the coverslips were observed using a LSM 510 confocal laser scanning microscope. Cells with the above treatments were fixed and visualized by immunofluorescence microscope using anti-β-III tubulin antibody (green channel) costained with antitubulinΔCsp6 antibody that specifically recognized caspase-6-cleaved tubulin (red channel). Nuclei were revealed by staining with DAPI. Merged images show the superimposition of both green and red signals, with overlapping areas appearing yellow. Aβ25–35 treatment induced caspase-mediated cleavage of tubulin, indicative of apoptosis. Source: Rhinacanthin C Alleviates Amyloid-β Fibrils' Toxicity on Neurons and Attenuates Neuroinflammation Triggered by LPS, Amyloid-β, and Interferon-γ in Glial Cells by Chuang, Kai-An et.al., Oxidative Medicine and Cellular Longevity, Oct. 2017.

Citations

View all 6 citations: Citation Explorer

High-frequency stimulation of the subthalamic nucleus activates motor cortex pyramidal tract neurons by a process involving local glutamate, GABA and dopamine receptors in hemi-parkinsonian rats
Authors: Chuang, Chi-Fen and Wu, Chen-Wei and Weng, Ying and Hu, Pei-San and Yeh, Shin-Rung and Chang, Yen-Chung
Journal: Chin. J. Physiol. (2018): 92--105

Rhinacanthin C alleviates amyloid-$\beta$ fibrils’ toxicity on neurons and attenuates neuroinflammation triggered by LPS, amyloid-$\beta$, and interferon-$\gamma$ in glial cells
Authors: Chuang, Kai-An and Li, Ming-Han and Lin, Ni-Hsuan and Chang, Chih-Hsuan and Lu, I and Pan, I and Takahashi, Tomoya and Perng, Ming-Der and Wen, Shu-Fang and others,
Journal: Oxidative Medicine and Cellular Longevity (2017)

Rhinacanthin C Alleviates Amyloid-β Fibrils' Toxicity on Neurons and Attenuates Neuroinflammation Triggered by LPS, Amyloid-β, and Interferon-γ in Glial Cells
Authors: Chuang, Kai-An and Li, Ming-Han and Lin, Ni-Hsuan and Chang, Chih-Hsuan and Lu, I and Pan, I and Takahashi, Tomoya and Perng, Ming-Der and Wen, Shu-Fang and others, undefined
Journal: Oxidative Medicine and Cellular Longevity (2017)

Impact of siRNA targeting of β-catenin on differentiation of rat neural stem cells and gene expression of Ngn1 and BMP4 following in vitro hypoxic-ischemic brain damage
Authors: Zhang, Xiaoying and Zhu, Cuicui and Luo, Qiong and Dong, Jv and Liu, Lv and Li, Min and Zhu, Hongtao and Ma, Xiangping and Wang, Jun
Journal: Molecular Medicine Reports (2016): 3595--3601

Application of interleukin-22 mediates protection in experimental acetaminophen-induced acute liver injury
Authors: Scheiermann, Patrick and Bachmann, Malte and Goren, Itamar and Zwissler, Bernhard and Pfeilschifter, Josef and Mühl, Heiko
Journal: The American journal of pathology (2013): 1107--1113

Synergistic activity of αvβ3 integrins and the elastin binding protein enhance cell-matrix interactions on bioactive hydrogel surfaces
Authors: Patel, Dhaval and V, undefined and romme, Susan E and Reid, Michael E and Taite, Lakeshia J
Journal: Biomacromolecules (2012): 1420--1428

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