iFluor® 350 PSA™ Imaging Kit with Goat Anti-Rabbit IgG

iFluor® 350 PSA™ Imaging Kit with Goat Anti-Rabbit IgG is a fluorescence signal amplification kit for sensitive detection of low-abundance targets in cells and tissues.

Enhanced Signal Amplification: PSA™ technology provides manufacturer-reported 10–50-fold higher fluorescence signals than conventional TSA reagents.
Spectral Properties: Excitation at 345 nm and emission at 450 nm.
HRP-Mediated Labeling: Enables localized covalent deposition of iFluor® 350 Styramide™ fluorophores.
Applications: Suitable for amplified immunofluorescence detection in cells and tissues.

Fluorescence images of HeLa cells labeled with rabbit anti-Tubulin primary antibody. Cells were then stained with a HRP-labeled Goat anti-Rabbit IgG secondary antibody followed by iFluor® 350 Styramide™ (Left) or Alexa Fluor® 350 tyramide (Middle and Right), respectively. Fluorescence images were taken using the DAPI filter set and the exposure time was labeled accordingly. iFluor® 350 Styramide™ shows significantly higher fluorescence intensity than Alexa Fluor® 350 tyramide if under the same exposure time (25 ms). Alexa Fluor® 350 tyramide requires exposure time higher (125 ms) to visualize the staining.

Protocol

SDS

Catalog	Size	Price	Quantity
45200	100 Tests	Price

Overview

Power Styramide™ Signal Amplification (PSA™) system is one of the most sensitive methods that can detect extremely low-abundance targets in cells and tissues with improved fluorescence signal 10-50 times higher than the widely used tyramide (TSA) reagents. In combination with our superior iFluor® dyes that have higher florescence intensity, increased photostability and enhanced water solubility, the iFluor® dye-labeled Styramide™ conjugates can generate fluorescence signal with significantly higher precision and sensitivity (more than 100 times) than standard ICC/IF/IHC. PSA™ utilizes the catalytic activity of horseradish peroxidase (HRP) for covalent deposition of fluorophores in situ. PSA™ radicals have much higher reactivity than tyramide radicals, making the PSA™ system much faster, more robust and sensitive than the traditional TSA reagents. Compared to tyramide reagents, the Styramide™ conjugates have ability to label the target at higher efficiency and thus generate significantly higher fluorescence signal. Styramide™ conjugates also allow significantly less consumption of primary antibody compared to standard directly conjugate method or tyramide amplification with the same level of sensitivity. iFluor® 350 PSA™ kit is a much superior replacement for Alexa Fluor 350 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.

Spectral properties

Correction factor (260 nm)	0.83
Correction factor (280 nm)	0.23
Extinction coefficient (cm ^-1 M ^-1)	20000 ¹
Excitation (nm)	345
Emission (nm)	450
Quantum yield	0.95 ¹

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microscope
Excitation	DAPI filter set
Emission	DAPI filter set
Recommended plate	Black wall/clear bottom

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 28, 2026

Component A: iFluor® 350 Styramide™ conjugate	1 vial (lyophilized powder)
Component B: Styramide™ Reaction Buffer	1 bottle (10 mL)
Component C: DMSO	1 vial (100 µL)
Component D: Secondary Antibody-HRP (Goat Anti-Rabbit IgG-HRP)	1 vial (100 µL) (100X)
Component E: Stabilized 3% Hydrogen Peroxide (H2O2)	1 vial (200 µL)