Name: iFluor® 647 PSA™ Imaging Kit with Goat Anti-Rabbit IgG
Brand: AAT Bioquest
SKU: 45240
Price: 594 USD
Availability: InStock

iFluor® 647 PSA™ Imaging Kit with Goat Anti-Rabbit IgG

iFluor® 647 PSA™ Imaging Kit with Goat Anti-Rabbit IgG is a far-red fluorescent signal amplification kit designed for sensitive detection of low-abundance targets in cells and tissues using rabbit primary antibodies.

Detection Principle: Uses HRP-mediated Styramide™ deposition for fluorescence signal amplification.
High Sensitivity: Generates 10–50 times stronger fluorescence signals than conventional tyramide reagents.
Spectral Properties: Excitation/emission maxima of 655/670 nm.
Applications: Suitable for immunocytochemistry, immunofluorescence, and immunohistochemistry.

Fluorescence IHC of formaldehyde-fixed, paraffin-embedded human lung adenocarcinoma positive tissue using PSA™ and TSA amplified methods. Human lung adenocarcinoma positive tissue sections were stained with rabbit anti-EpCaM antibody and then incubated with poly HRP-labeled Goat anti-Rabbit IgG secondary antibody followed by iFluor® 647 Styramide™ (Cat#45240) or Alexa Fluor® 647 tyramide stain respectively. Images showed that iFluor® 647 PSA™ super signal amplification can increase the sensitivity of fluorescence IHC over Alexa Fluor® 647 TSA method.

Protocol

SDS

Catalog	Size	Price	Quantity
45240	100 Tests	Price

Overview

Power Styramide™ Signal Amplification (PSA™) system is one of the most sensitive methods that can detect extremely low-abundance targets in cells and tissues with improved fluorescence signal 10-50 times higher than the widely used tyramide (TSA) reagents. In combination with our superior iFluor® dyes that have higher florescence intensity, increased photostability and enhanced water solubility, the iFluor® dye-labeled Styramide™ conjugates can generate fluorescence signal with significantly higher precision and sensitivity (more than 100 times) than standard ICC/IF/IHC. PSA™ utilizes the catalytic activity of horseradish peroxidase (HRP) for covalent deposition of fluorophores in situ. PSA™ radicals have much higher reactivity than tyramide radicals, making the PSA™ system much faster, more robust and sensitive than the traditional TSA reagents. Compared to tyramide reagents, the Styramide™ conjugates have ability to label the target at higher efficiency and thus generate significantly higher fluorescence signal. Styramide™ conjugates also allow significantly less consumption of primary antibody compared to standard directly conjugate method or tyramide amplification with the same level of sensitivity. iFluor® 647 PSA™ kit is a much superior replacement for Alexa Fluor 647 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.

Spectral properties

Correction factor (260 nm)	0.03
Correction factor (280 nm)	0.03
Correction factor (656 nm)	0.0793
Extinction coefficient (cm ^-1 M ^-1)	220000 ¹
Excitation (nm)	655
Emission (nm)	670
Quantum yield	0.25 ¹

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microscope
Excitation	Cy5 filter set
Emission	Cy5 filter set
Recommended plate	Black wall/clear bottom

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 28, 2026

Component A: iFluor® 647 Styramide™ conjugate	1 vial (lyophilized powder)
Component B: Styramide™ Reaction Buffer	1 bottle (10 mL)
Component C: DMSO	1 vial (100 µL)
Component D: Secondary Antibody-HRP (Goat Anti-Rabbit IgG-HRP)	1 vial (100 µL) (100X)
Component E: Stabilized 3% Hydrogen Peroxide (H2O2)	1 vial (200 µL)