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iFluor® 594 PSA™ Imaging Kit with Goat Anti-Rabbit IgG
Power Styramide™ Signal Amplification (PSA™) system is one of the most sensitive methods that can detect extremely low-abundance targets in cells and tissues with improved fluorescence signal 10-50 times higher than the widely used tyramide (TSA) reagents. In combination with our superior iFluor® dyes that have higher florescence intensity, increased photostability and enhanced water solubility, the iFluor® dye-labeled Styramide™ conjugates can generate fluorescence signal with significantly higher precision and sensitivity (more than 100 times) than standard ICC/IF/IHC. PSA utilizes the catalytic activity of horseradish peroxidase (HRP) for covalent deposition of fluorophores in situ. PSA radicals have much higher reactivity than tyramide radicals, making the PSA system much faster, more robust and sensitive than the traditional TSA reagents. Compared to tyramide reagents, the Styramide™ conjugates have ability to label the target at higher efficiency and thus generate significantly higher fluorescence signal. Styramide™ conjugates also allow significantly less consumption of primary antibody compared to standard directly conjugate method or tyramide amplification with the same level of sensitivity. iFluor® 594 PSA kit is a much superior replacement for Alexa Fluor 594 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.
Fluorescence IHC in formaldehyde-fixed paraffin-embedded tissue. Human lung adenocarcinoma sections were incubated with Rabbit mAb EpCAM then stained a HRP-labeled Goat anti-Rabbit IgG secondary antibody followed by iFluor® 594 Styramide™ (Left) or Alexa Fluor® 594 tyramide (Right), respectively. Fluorescence images were taken using the TRITC filter set and under the same exposure time. Cell nucleus was stained with Nuclear Blue™ DCS1 (Cat#17548).
Fluorescence IHC in formaldehyde-fixed paraffin-embedded tissue. Human lung adenocarcinoma sections were incubated with Rabbit mAb EpCAM then stained a HRP-labeled Goat anti-Rabbit IgG secondary antibody followed by iFluor® 594 Styramide™ (Left) or Alexa Fluor® 594 tyramide (Right), respectively. Fluorescence images were taken using the TRITC filter set and under the same exposure time. Cell nucleus was stained with Nuclear Blue™ DCS1 (Cat#17548).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
45230100 Tests
Price
 
Spectral properties
Absorbance (nm)587
Correction factor (260 nm)0.05
Correction factor (280 nm)0.04
Extinction coefficient (cm -1 M -1)
200000
1
Excitation (nm)587
Emission (nm)603
Quantum yield
0.53
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microscope
ExcitationCy3/TRITC filter set
EmissionCy3/TRITC filter set
Recommended plateBlack wall/clear bottom
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Page updated on October 8, 2025