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iFluor® 488 PSA™ Imaging Kit with Goat Anti-Rabbit IgG
Power Styramide™ Signal Amplification (PSA™) system is one of the most sensitive methods that can detect extremely low-abundance targets in cells and tissues with improved fluorescence signal 10-50 times higher than the widely used tyramide (TSA) reagents. In combination with our superior iFluor® dyes that have higher florescence intensity, increased photostability and enhanced water solubility, the iFluor® dye-labeled Styramide™ conjugates can generate fluorescence signal with significantly higher precision and sensitivity (more than 100 times) than standard ICC/IF/IHC. PSA utilizes the catalytic activity of horseradish peroxidase (HRP) for covalent deposition of fluorophores in situ. PSA radicals have much higher reactivity than tyramide radicals, making the PSA system much faster, more robust and sensitive than the traditional TSA reagents. Compared to tyramide reagents, the Styramide™ conjugates have ability to label the target at higher efficiency and thus generate significantly higher fluorescence signal. Styramide™ conjugates also allow significantly less consumption of primary antibody compared to standard directly conjugate method or tyramide amplification with the same level of sensitivity. iFluor® 488 PSA kit is a much superior replacement for Alexa Fluor 488 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.
Fluorescence IHC of formaldehyde-fixed, paraffin-embedded human lung adenocarcinoma using two imaging signal amplification kits. Tissue sections were stained with rabbit anti-EpCam antibody and then followed PSA™ method using iFluor® 488 PSA™ Imaging Kit with goat anti-rabbit IgG (Cat#45205) or Thermo Fisher Alexa Fluor® 488 Superboost Kit  respectively. Cell nucleus were stained with Nuclear Blue™ DCS1 (Cat#17548).
Fluorescence IHC of formaldehyde-fixed, paraffin-embedded human lung adenocarcinoma using two imaging signal amplification kits. Tissue sections were stained with rabbit anti-EpCam antibody and then followed PSA™ method using iFluor® 488 PSA™ Imaging Kit with goat anti-rabbit IgG (Cat#45205) or Thermo Fisher Alexa Fluor® 488 Superboost Kit  respectively. Cell nucleus were stained with Nuclear Blue™ DCS1 (Cat#17548).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
45205100 Tests
Price
 
Spectral properties
Correction factor (260 nm)0.21
Correction factor (280 nm)0.11
Extinction coefficient (cm -1 M -1)
75000
1
Excitation (nm)491
Emission (nm)516
Quantum yield
0.9
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microscope
ExcitationFITC filter set
EmissionFITC filter set
Recommended plateBlack wall/clear bottom
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Page updated on October 5, 2025