iFluor® 488 PSA™ Imaging Kit with Goat Anti-Rabbit IgG

iFluor® 488 PSA™ Imaging Kit with Goat Anti-Rabbit IgG enables amplified fluorescent detection of low-abundance targets in cells and tissues using rabbit primary antibodies.

Enhanced Signal Amplification: PSA™ technology provides manufacturer-reported 10–50-fold higher fluorescence signals than conventional TSA reagents.
Spectral Properties: Excitation at 491 nm and emission at 516 nm.
HRP-Mediated Labeling: Enables localized covalent deposition of iFluor® 488 Styramide™ fluorophores.
Applications: Suitable for amplified immunofluorescence and immunohistochemical staining of cells and tissues.

Fluorescence IHC of formaldehyde-fixed, paraffin-embedded human lung adenocarcinoma using two imaging signal amplification kits. Tissue sections were stained with rabbit anti-EpCam antibody and then followed PSA™ method using iFluor® 488 PSA™ Imaging Kit with goat anti-rabbit IgG (Cat#45205) or Thermo Fisher Alexa Fluor® 488 Superboost Kit  respectively. Cell nucleus were stained with Nuclear Blue™ DCS1 (Cat#17548).

Protocol

SDS

Catalog	Size	Price	Quantity
45205	100 Tests	Price

Overview

Power Styramide™ Signal Amplification (PSA™) system is one of the most sensitive methods that can detect extremely low-abundance targets in cells and tissues with improved fluorescence signal 10-50 times higher than the widely used tyramide (TSA) reagents. In combination with our superior iFluor® dyes that have higher florescence intensity, increased photostability and enhanced water solubility, the iFluor® dye-labeled Styramide™ conjugates can generate fluorescence signal with significantly higher precision and sensitivity (more than 100 times) than standard ICC/IF/IHC. PSA™ utilizes the catalytic activity of horseradish peroxidase (HRP) for covalent deposition of fluorophores in situ. PSA™ radicals have much higher reactivity than tyramide radicals, making the PSA™ system much faster, more robust and sensitive than the traditional TSA reagents. Compared to tyramide reagents, the Styramide™ conjugates have ability to label the target at higher efficiency and thus generate significantly higher fluorescence signal. Styramide™ conjugates also allow significantly less consumption of primary antibody compared to standard directly conjugate method or tyramide amplification with the same level of sensitivity. iFluor® 488 PSA™ kit is a much superior replacement for Alexa Fluor 488 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.

Spectral properties

Correction factor (260 nm)	0.21
Correction factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	75000 ¹
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.9 ¹

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microscope
Excitation	FITC filter set
Emission	FITC filter set
Recommended plate	Black wall/clear bottom

Telephone
Fax
Email	sales@aatbio.com
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Technical support	Contact us
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Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 28, 2026

Component A: iFluor® 488 Styramide™ conjugate	1 vial (lyophilized powder)
Component B: Styramide™ Reaction Buffer	1 bottle (10 mL)
Component C: DMSO	1 vial (100 µL)
Component D: Secondary Antibody-HRP (Goat Anti-Rabbit IgG-HRP)	1 vial (100 µL) (100X)
Component E: Stabilized 3% Hydrogen Peroxide (H2O2)	1 vial (200 µL)