Name: iFluor® 350 PSA™ Imaging Kit with Goat Anti-Mouse IgG
Brand: AAT Bioquest
SKU: 45250
Price: 594 USD
Availability: InStock

iFluor® 350 PSA™ Imaging Kit with Goat Anti-Mouse IgG

iFluor® 350 PSA™ Imaging Kit with Goat Anti-Mouse IgG is a fluorescence signal amplification kit for sensitive detection of low-abundance targets in cells and tissues.

Enhanced Signal Amplification: PSA™ technology delivers manufacturer-reported 10–50-fold stronger fluorescence signals than conventional TSA reagents.
Spectral Properties: Excitation at 345 nm and emission at 450 nm.
HRP-Mediated Labeling: Catalyzes covalent deposition of iFluor® 350 Styramide™ at target sites.
Applications: Suitable for amplified immunofluorescence detection in cells and tissues.

Power Styramide™ Signal Amplification (PSA™) system is one of the most sensitive methods that can detect extremely low-abundance targets in cells and tissues with improved fluorescence signal 10-50 times higher than the widely used tyramide (TSA) reagents. In combination with our superior iFluor® dyes that have higher florescence intensity, increased photostability and enhanced water solubility, the iFluor® dye-labeled Styramide™ conjugates can generate fluorescence signal with significantly higher precision and sensitivity (more than 100 times) than standard ICC/IF/IHC. PSA utilizes the catalytic activity of horseradish peroxidase (HRP) for covalent deposition of fluorophores in situ.  PSA radicals have much higher reactivity than tyramide radicals, making the PSA system much faster, more robust and sensitive than the traditional TSA reagents.

Protocol

SDS

Catalog	Size	Price	Quantity
45250	100 Tests	Price

Overview

Power Styramide™ Signal Amplification (PSA™) system is one of the most sensitive methods that can detect extremely low-abundance targets in cells and tissues with improved fluorescence signal 10-50 times higher than the widely used tyramide (TSA) reagents. In combination with our superior iFluor® dyes that have higher florescence intensity, increased photostability and enhanced water solubility, the iFluor® dye-labeled Styramide™ conjugates can generate fluorescence signal with significantly higher precision and sensitivity (more than 100 times) than standard ICC/IF/IHC. PSA™ utilizes the catalytic activity of horseradish peroxidase (HRP) for covalent deposition of fluorophores in situ. PSA™ radicals have much higher reactivity than tyramide radicals, making the PSA™ system much faster, more robust and sensitive than the traditional TSA reagents. Compared to tyramide reagents, the Styramide™ conjugates have ability to label the target at higher efficiency and thus generate significantly higher fluorescence signal. Styramide™ conjugates also allow significantly less consumption of primary antibody compared to standard directly conjugate method or tyramide amplification with the same level of sensitivity. iFluor® 350 PSA™ kit is a much superior replacement for Alexa Fluor 350 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.

Spectral properties

Correction factor (260 nm)	0.83
Correction factor (280 nm)	0.23
Extinction coefficient (cm ^-1 M ^-1)	20000 ¹
Excitation (nm)	345
Emission (nm)	450
Quantum yield	0.95 ¹

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microscope
Excitation	DAPI filter set
Emission	DAPI filter set
Recommended plate	Black wall/clear bottom

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 28, 2026

Component A: iFluor® 350 Styramide™ conjugate	1 vial (lyophilized powder)
Component B: Styramide™ Reaction Buffer	1 bottle (10 mL)
Component C: DMSO	1 vial (100 µL)
Component D: Secondary Antibody-HRP (Goat Anti-Mouse IgG-HRP)	1 vial (100 µL) (100X)
Component E: Stabilized 3% Hydrogen Peroxide (H2O2)	1 vial (200 µL)