iFluor® 647 amine
AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, and 633 nm). iFluor® 647 dyes have fluorescence excitation and emission maxima of ~656 nm and ~670 nm respectively. iFluor® 647 family has spectral properties essentially identical to those of Cy5®. Compared to Cy5® probes, iFluor® 647 reagents have much stronger fluorescence and higher photostability. Their fluorescence is pH-independent from pH 3 to 11. These spectral characteristics make this new dye family an excellent alternative to Cy5® and Alexa Fluor® 647 (Cy5® and Alexa Fluor® are the trademarks of GE Health Care and Invitrogen). iFluor® 647 amine is stable and used for modifying carbonyl groups (e.g., aldehyde and carboxy groups).
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 647 amine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 97.94 µL | 489.702 µL | 979.403 µL | 4.897 mL | 9.794 mL |
5 mM | 19.588 µL | 97.94 µL | 195.881 µL | 979.403 µL | 1.959 mL |
10 mM | 9.794 µL | 48.97 µL | 97.94 µL | 489.702 µL | 979.403 µL |
Molarity calculator
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Spectrum
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Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
iFluor® 350 amine | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
iFluor® 405 amine | 403 | 427 | 370001 | 0.911 | 0.48 | 0.77 |
iFluor® 488 amine | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 555 amine | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
iFluor® 660 amine | 663 | 678 | 2500001 | 0.261 | 0.07 | 0.08 |
iFluor® 680 amine | 684 | 701 | 2200001 | 0.231 | 0.097 | 0.094 |
iFluor® 700 amine | 690 | 713 | 2200001 | 0.231 | 0.09 | 0.04 |
iFluor® 710 amine | 716 | 739 | 1500001 | 0.601 | 0.12 | 0.07 |
iFluor® 750 amine | 757 | 779 | 2750001 | 0.121 | 0.044 | 0.039 |
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Citations
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Journal: Journal of Inflammation (2024): 1--13
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Journal: (2024)
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Authors: Liu, Feng and Xin, Minhang and Feng, Huiheng and Zhang, Wentao and Liao, Ziyan and Sheng, Tao and Wen, Ping and Wu, Qing and Liang, Tingxizi and Shi, Jiaqi and others,
Journal: Science Advances (2024): eadk8264
Authors: Liu, Feng and Xin, Minhang and Feng, Huiheng and Zhang, Wentao and Liao, Ziyan and Sheng, Tao and Wen, Ping and Wu, Qing and Liang, Tingxizi and Shi, Jiaqi and others,
Journal: Science Advances (2024): eadk8264
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Authors: Y{\"u}ksel, B{\"u}{\c{s}}ra and T{\"u}rkel, Nezaket and {\c{S}}ahin, Fikrettin and DENIZ, ASLI AYSEN HIZLI
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Authors: Y{\"u}ksel, B{\"u}{\c{s}}ra and T{\"u}rkel, Nezaket and {\c{S}}ahin, Fikrettin and DENIZ, ASLI AYSEN HIZLI
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References
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