iFluor® 647 amine

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	1021.03
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.03
Correction Factor (280 nm)	0.03
Correction Factor (656 nm)	0.0793
Extinction coefficient (cm ^-1 M ^-1)	250000¹
Excitation (nm)	656
Emission (nm)	670
Quantum yield	0.25¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Alternative formats

Overview SDS Protocol

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 647 amine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	97.94 µL	489.702 µL	979.403 µL	4.897 mL	9.794 mL
5 mM	19.588 µL	97.94 µL	195.881 µL	979.403 µL	1.959 mL
10 mM	9.794 µL	48.97 µL	97.94 µL	489.702 µL	979.403 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Correction Factor (260 nm)	0.03
Correction Factor (280 nm)	0.03
Correction Factor (656 nm)	0.0793
Extinction coefficient (cm ^-1 M ^-1)	250000¹
Excitation (nm)	656
Emission (nm)	670
Quantum yield	0.25¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
iFluor® 350 amine	345	450	20000¹	0.95¹	0.83	0.23
iFluor® 405 amine	403	427	37000¹	0.91¹	0.48	0.77
iFluor® 488 amine	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 555 amine	557	570	100000¹	0.64¹	0.23	0.14
iFluor® 660 amine	663	678	250000¹	0.26¹	0.07	0.08
iFluor® 680 amine	684	701	220000¹	0.23¹	0.097	0.094
iFluor® 700 amine	690	713	220000¹	0.23¹	0.09	0.04
iFluor® 710 amine	717	739	190000¹	0.60¹	0.12	0.07
iFluor® 750 amine	757	779	275000¹	0.12¹	0.044	0.039
iFluor® 790 amine	787	812	250000¹	0.13¹	0.1	0.09
iFluor® 647 Styramide Superior Replacement for Alexa Fluor 647 tyramide	656	670	250000¹	0.25¹	0.03	0.03
iFluor® 647 Tyramide	656	670	250000¹	0.25¹	0.03	0.03
iFluor® 647 TCO	656	670	250000¹	0.25¹	0.03	0.03
iFluor® 647 Tetrazine	656	670	250000¹	0.25¹	0.03	0.03
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Images

Figure 1. Fluorescent dye amines are the most popular tool for conjugating dyes to a target compound with a carbonyl group (e.g., aldehyde, carboxylic acid or activated carboxy group such as NHS ester).

Citations

View all 17 citations: Citation Explorer

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Authors: Lightley, Jonathan and Kumar, Sunil and Lim, MQ and Garcia, E and G{\"o}rlitz, F and Alexandrov, Y and Parrado, T and Hollick, C and Steele, E and Ro{\ss}mann, K and others,
Journal: Journal of Microscopy (2023)

Procyanidin A1 acts as an antioxidant stressor in gestational diabetes
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Journal: Tropical Journal of Pharmaceutical Research (2023): 1863--1869

Inhibition of MYC suppresses programmed cell death ligand-1 expression and enhances immunotherapy in triple-negative breast cancer
Authors: Li, Xintong and Tang, Lin and Chen, Qin and Cheng, Xumin and Liu, Yiqiu and Wang, Cenzhu and Zhu, Chengjun and Xu, Kun and Gao, Fangyan and Huang, Jinyi and others,
Journal: Chinese Medical Journal (2022): 10--1097

Immune-regulating bimetallic metal-organic framework nanoparticles designed for cancer immunotherapy
Authors: Dai, Zan and Wang, Qiaoyun and Tang, Jie and Wu, Min and Li, Haoze and Yang, Yannan and Zhen, Xu and Yu, Chengzhong
Journal: Biomaterials (2022): 121261

Site-specific labeling and functional efficiencies of human fibroblast growth Factor-1 with a range of fluorescent Dyes in the flexible N-Terminal region and a rigid $\beta$-turn region
Authors: Mohale, Mamello and Gundampati, Ravi Kumar and Kumar, Thallapuranam Krishnaswamy Suresh and Heyes, Colin D
Journal: Analytical biochemistry (2022): 114524

Poly ADP-ribosylation of SET8 leads to aberrant H4K20 methylation in mammalian nuclear genome
Authors: Est{\`e}ve, Pierre-Olivier and Sen, Sagnik and Vishnu, Udayakumar S and Ruse, Cristian and Chin, Hang Gyeong and Pradhan, Sriharsa
Journal: Communications biology (2022): 1--18

The PARP1 Inhibitor Niraparib Represses DNA Damage Repair and Synergizes with Temozolomide for Antimyeloma Effects
Authors: Shen, Hong-Yuan and Tang, Hai-Long and Zheng, Yan-Hua and Feng, Juan and Dong, Bao-Xia and Chen, Xie-Qun
Journal: Journal of Oncology (2022)

A Substance P (SP)/Neurokinin-1 Receptor Axis Promotes Perineural Invasion of Pancreatic Cancer and Is Affected by lncRNA LOC389641
Authors: Ji, Tengfei and Ma, Keqiang and Wu, Hongsheng and Cao, Tiansheng
Journal: Journal of Immunology Research (2022)

Digital Light Processing 3D Printing of Gyroid Scaffold with Isosorbide-Based Photopolymer for Bone Tissue Engineering
Authors: Verisqa, Fiona and Cha, Jae-Ryung and Nguyen, Linh and Kim, Hae-Won and Knowles, Jonathan C
Journal: Biomolecules (2022): 1692

SP/NK-1R Axis Promotes Perineural Invasion of Pancreatic Cancer and is Affected by lncRNA LOC389641
Authors: Ji, Tengfei and Ma, Keqiang and Wu, Hongsheng and Cao, Tiansheng
Journal: (2021)

References

View all 49 references: Citation Explorer

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Journal: J Microbiol Methods (2011): 119

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