iFluor® Ultra 594 succinimidyl ester

Fluorescent dye-conjugated antibodies provide a tool for identifying proteins in many applications including fluorescent cell imaging, flow cytometry, western blotting, immunohistochemistry and more. The advantages of using a fluorescently labeled antibody include higher sensitivity, multiplexing capabilities, and ease of use. iFluor® Ultra family is a recent upgrade of our popular iFluor® dyes and optimized for labeling antibodies used for fluorescence imaging and flow cytometry applications. Antibody conjugates prepared with iFluor® Ultra 594 are far superior to the conjugates of other existing similar dyes such as Alexa Fluor® 594. iFluor® Ultra 594 conjugates are significantly brighter than the conjugates prepared with Alexa Fluor® 594 under the same conditions. Additionally, the fluorescence of iFluor® Ultra 594 is not affected by pH (4-10). iFluor® Ultra 594 SE dye is reasonably stable and shows good reactivity and selectivity with protein amino groups. iFluor® Ultra 594 has spectral properties and reactivity similar to Alexa Fluor® 594 (Alexa Fluor® is the trademark of ThermoFisher).

HeLa cells were incubated with mouse anti-tubulin followed by AAT’s iFluor<sup>TM</sup> Ultra 594 goat anti-mouse IgG conjugate or Alexa Fluor<sup>®</sup> 594 goat anti-mouse IgG.

Protocol

COA

Catalog	Size	Price
71650	1 mg	Price
71651	100 ug	Price
71652	5 mg	Price

References

View all 50 references: Citation Explorer

MicroRNA-126 inhibits pathological retinal neovascularization via suppressing vascular endothelial growth factor expression in a rat model of retinopathy of prematurity.

Authors:

Fan, Yuan-Yao and Liu, Chi-Hsien and Wu, An-Lun and Chen, Hung-Chi and Hsueh, Yi-Jen and Chen, Kuan-Jen and Lai, Chi-Chun and Huang, Chung-Ying and Wu, Wei-Chi

Journal:

European journal of pharmacology (2021): 174035

Retinal ganglion cells projecting to superior colliculus and pulvinar in marmoset.

Authors:

Grünert, Ulrike and Lee, Sammy C S and Kwan, William C and Mundinano, Inaki-Carril and Bourne, James A and Martin, Paul R

Journal:

Brain structure & function (2021)

A fully integrated isotachophoresis with a programmable microfluidic platform.

Authors:

Shebindu, Adam and Somaweera, Himali and Estlack, Zachary and Kim, Jungtae and Kim, Jungkyu

Journal:

Talanta (2021): 122039

Liquid Droplet Formation and Facile Cytosolic Translocation of IgG in the Presence of Attenuated Cationic Amphiphilic Lytic Peptides.

Authors:

Iwata, Takahiro and Hirose, Hisaaki and Sakamoto, Kentarou and Hirai, Yusuke and Arafiles, Jan Vincent V and Akishiba, Misao and Imanishi, Miki and Futaki, Shiroh

Journal:

Angewandte Chemie (International ed. in English) (2021)

Effect of VIRP1 Protein on Nuclear Import of Citrus Exocortis Viroid (CEVd).

Authors:

Seo, Hyesu and Kim, Kyunghee and Park, Woong June

Journal:

Biomolecules (2021)

Physical properties

Molecular weight	1436.72
Solvent	DMSO

Spectral properties

Absorbance (nm)	585
Correction factor (260 nm)	0.07
Correction factor (280 nm)	0.05
Extinction coefficient (cm ^-1 M ^-1)	180000 ¹
Excitation (nm)	586
Emission (nm)	600
Quantum yield	0.52 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on October 8, 2025