iFluor™ 647 succinimidyl ester

Save 25% on your purchase of iFluor™ 647 succinimidyl ester when you use the promotion code ifluor25. Act quickly, offer expires 05-31-19.Details

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<p>HeLa cells were stained with mouse anti-tubulin followed with iFluor<sup>TM</sup> 647 goat anti-mouse IgG (H+L) (red); and nuclei were stained with DAPI (blue).</p>
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Unit Size: Cat No: Price (USD): Qty:
1 mg 1031 $195


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Additional Ordering Information
Telephone: 1-800-990-8053
Fax: 1-408-733-1304
Email: sales@aatbio.com
International: See distributors





Overview

Ex/Em (nm)649/664
MW1050.35
SolventDMSO
Storage Freeze (<-15 °C)
Minimize light exposure
Category Superior Labeling Dyes
iFluor Dyes and Kits
Related Amine-Reactive Probes
Labeling via Amino Groups
AAT Bioquest's iFluor™ dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). iFluor™ 647 family has the spectral properties essentially identical to those of Cy5® (Cy5® is the trademark of GE Healthcare). Compared to Cy5 probes iFluor™ 647 family has much stronger fluorescence and higher photostability. Their fluorescence is pH-independent from pH 3 to 11. These spectral characteristics make this new dye family an excellent alternative to Cy5® and Alexa Fluor® 647 (Cy5® and Alexa Fluor® are the trademark of Invitrogen and GE Health Care) . iFluor™ 647 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups.




Calculators
Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor™ 647 succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.



Molarity calculator

Table 2. Enter any two values (mass, volume, concentration) to calculate the third.

Mass Molecular weight Volume Concentration Moles
/ = x =
 






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References & Citations

Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification
Authors: D Gao, N Liu, Y Li, Y Zhang, G Liu
Journal: Metabolomics (Los Angel) (2017): 2153--0769

Suramin inhibits cullin-RING E3 ubiquitin ligases
Authors: Kenneth Wu, Robert A Chong, Qing Yu, Jin Bai, Donald E Spratt, Kevin Ching, Chan Lee, Haibin Miao, Inger Tappin, Jerard Hurwitz
Journal: Proceedings of the National Academy of Sciences (2016): E2011--E2018

Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function
Authors: Helene Piccard, Nele Berghmans, Eva Korpos, Chris Dillen, Ilse Van Aelst, Sandra Li, Erik Martens, Sandra Liekens, Sam Noppen, Jo Van Damme
Journal: International Journal of Cancer (2012): E425--E436






Additional Documents

 
Safety Data Sheet (SDS)


Catalogs
1. Fluorescent Labeling Probes & Kits

Application Notes
1. AssayWise Letters 2012, Vol 1(1)

Certificate of Analysis