iFluor® 555 Tetrazine
The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This has gained popularity due to its extremely fast kinetics. AAT Bioquest offers a group of tetrazine- and TCO-containing dyes for exploring various biological systems that can use this powerful click reaction. iFluor® 555 tetrazine can be readily used to label TCO-modified biological molecules for fluorescence imaging and other fluorescence-based biological applications. The peptide and oligo conjugates prepared with iFluor® 555 dye are far superior to the conjugates of other spectrally similar dyes, such as the popular Cy3 and Alexa Fluor® 555. iFluor® 555 conjugates are significantly brighter and much more photostable than the corresponding conjugates of Cy3 and Alexa Fluor® 555 (Alexa Fluor® is the trademark of Invitrogen).
Spectrum
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Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
iFluor® 555 maleimide | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
iFluor® 555 amine | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
iFluor® 555 hydrazide | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
iFluor® 555 alkyne | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
iFluor® 555 azide | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
iFluor® 555 Styramide *Superior Replacement for Alexa Fluor 555 tyramide and Opal 570* | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
iFluor® 555 Tyramide | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
iFluor® 555 TCO | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
iFluor® 488 Tetrazine | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
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References
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