Name: iFluor® 350 Styramide™
Brand: AAT Bioquest
SKU: 45000
Price: 355 USD
Availability: InStock

iFluor® 350 Styramide™

iFluor® 350 Styramide™ is a blue-fluorescent signal amplification reagent for sensitive detection of low-abundance targets in cells and tissues using HRP-mediated labeling.

Enhanced Signal Amplification: PSA™ technology provides manufacturer-reported 10–50-fold stronger fluorescence signals than conventional TSA reagents.
Spectral Properties: Excitation at 345 nm and emission at 450 nm.
HRP-Mediated Labeling: Enables localized covalent deposition of fluorescent labels at target sites.
Filter Compatibility: Supports fluorescence detection using standard DAPI filter sets.
Applications: Suitable for amplified immunofluorescence and immunohistochemical staining of cells and tissues.

Formalin-fixed, paraffin-embedded (FFPE) human lung tissue was labeled with anti-EpCAM mouse mAb followed by HRP-labeled goat anti-mouse IgG (Cat No. 16728). The fluorescence signal was developed using iFluor® 350 styramide (Cat No. 45000) and detected with a DAPI filter set.

Protocol

SDS

Catalog	Size	Price	Quantity
45000	100 Slides	Price

Overview

Power Styramide™ Signal Amplification (PSA™) system is one of the most sensitive methods that can detect extremely low-abundance targets in cells and tissues with improved fluorescence signal 10-50 times higher than the widely used tyramide (TSA) reagents. In combination with our superior iFluor® dyes that have higher florescence intensity, increased photostability and enhanced water solubility, the iFluor® dye-labeled Styramide™ conjugates can generate fluorescence signal with significantly higher precision and sensitivity (more than 100 times) than standard ICC/IF/IHC. PSA™ utilizes the catalytic activity of horseradish peroxidase (HRP) for covalent deposition of fluorophores in situ. PSA™ radicals have much higher reactivity than tyramide radicals, making the PSA™ system much faster, more robust and sensitive than the traditional TSA reagents. Compared to tyramide reagents, the Styramide™ conjugates have ability to label the target at higher efficiency and thus generate significantly higher fluorescence signal. Styramide™ conjugates also allow significantly less consumption of primary antibody compared to standard directly conjugate method or tyramide amplification with the same level of sensitivity. iFluor® 350 Styramide is a superior replacement for Alexa Fluor 350 tyramide or other spectrally similar fluorescent tyramide conjugates or TSA reagents.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 350 Styramide™ to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	107.396 µL	536.982 µL	1.074 mL	5.37 mL	10.74 mL
5 mM	21.479 µL	107.396 µL	214.793 µL	1.074 mL	2.148 mL
10 mM	10.74 µL	53.698 µL	107.396 µL	536.982 µL	1.074 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	931.13 g/mol	=	mL	x	mM	=	mmol

Physical properties

Molecular weight	931.13
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.83
Correction factor (280 nm)	0.23
Extinction coefficient (cm ^-1 M ^-1)	20000 ¹
Excitation (nm)	345
Emission (nm)	450
Quantum yield	0.95 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

Fluorescence microscope
Excitation	DAPI filter set
Emission	DAPI filter set
Recommended plate	Black wall/clear bottom

Telephone
Fax
Email	sales@aatbio.com
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Purchase order	Send to sales@aatbio.com
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Page updated on October 30, 2026