iFluor® Ultra 647 succinimidyl ester

Fluorescent dye-conjugated antibodies provide a tool for identifying proteins in many applications including fluorescent cell imaging, flow cytometry, western blotting, immunohistochemistry and more. The advantages of using a fluorescently labeled antibody include higher sensitivity, multiplexing capabilities, and ease of use. iFluor® Ultra family is a recent upgrade of our popular iFluor® dyes and optimized for labeling antibodies used for fluorescence imaging and flow cytometry applications. Antibody conjugates prepared with iFluor® Ultra 647 are far superior to the conjugates of other existing similar dyes such as Cy5, Dylight 650 and Alexa Fluor® 647. iFluor® Ultra 647 conjugates are significantly brighter than the conjugates prepared with Cy5, Dylight 650 and Alexa Fluor® 647 under the same conditions. Additionally, the fluorescence of iFluor® Ultra 647 is not affected by pH (4-10). iFluor® Ultra 647 SE dye is reasonably stable and shows good reactivity and selectivity with protein amino groups. iFluor® Ultra 647 has spectral properties and reactivity similar to Cy5, Dylight 650 and Alexa Fluor® 647 (Cy5® and Alexa Fluor® is the trademarks of GE Healthcare and ThermoFisher respectively).

HeLa cells were incubated with mouse anti-tubulin followed by AAT’s iFluor<sup>TM</sup> Ultra 647 goat anti-mouse IgG conjugate or Alexa Fluor<sup>®</sup> 647 goat anti-mouse IgG.

Protocol

COA

Catalog	Size	Price
71670	1 mg	Price
71671	100 ug	Price
71672	5 mg	Price

Citations

View all 5 citations: Citation Explorer

Site-specific labeling and functional efficiencies of human fibroblast growth Factor-1 with a range of fluorescent Dyes in the flexible N-Terminal region and a rigid $\beta$-turn region

Authors:

Mohale, Mamello and Gundampati, Ravi Kumar and Kumar, Thallapuranam Krishnaswamy Suresh and Heyes, Colin D

Journal:

Analytical biochemistry (2022): 114524

SP/NK-1R Axis Promotes Perineural Invasion of Pancreatic Cancer and is Affected by lncRNA LOC389641

Authors:

Ji, Tengfei and Ma, Keqiang and Wu, Hongsheng and Cao, Tiansheng

Pharmacological targeting of Sam68 functions in colorectal cancer stem cells

Authors:

Masibag, Angelique N and Bergin, Christopher J and Haebe, Joshua R and Zouggar, A{\"\i}cha and Shah, Muhammad S and Sandouka, Tamara and da Silva, Amanda Mendes and Desrochers, Fran{\c{c}}ois M and Fournier-Morin, Aube and Benoit, Yannick D

Journal:

Iscience (2021): 103442

Efferocytosis induces macrophage proliferation to help resolve tissue injury

Authors:

Gerlach, Brennan D and Ampomah, Patrick B and Yurdagul Jr, Arif and Liu, Chuang and Lauring, Max C and Wang, Xiaobo and Kasikara, Canan and Kong, Na and Shi, Jinjun and Tao, Wei and others,

Journal:

Cell metabolism (2021): 2445--2463

Influence of particle geometry on gastrointestinal transit and absorption following oral administration

Authors:

Li, Dong and Zhuang, Jie and He, Haisheng and Jiang, Sifan and Banerjee, Amrita and Lu, Yi and Wu, Wei and Mitragotri, Samir and Gan, Li and Qi, Jianping

Journal:

ACS applied materials \& interfaces (2017): 42492--42502

References

View all 50 references: Citation Explorer

SMALL MOLECULE IMAGING AGENT FOR MUTANT KRAS G12C.

Authors:

Koch, Peter D and Quintana, Jeremy and Ahmed, Maaz and Kohler, Rainer H and Weissleder, Ralph

Journal:

Advanced therapeutics (2021)

Delivery of functional exogenous proteins by plant-derived vesicles to human cells in vitro.

Authors:

Garaeva, Luiza and Kamyshinsky, Roman and Kil, Yury and Varfolomeeva, Elena and Verlov, Nikolai and Komarova, Elena and Garmay, Yuri and Landa, Sergey and Burdakov, Vladimir and Myasnikov, Alexander and Vinnikov, Ilya A and Margulis, Boris and Guzhova, Irina and Kagansky, Alexander and Konevega, Andrey L and Shtam, Tatiana

Journal:

Scientific reports (2021): 6489

Effect of membrane potential on entry of lactoferricin B-derived 6-residue antimicrobial peptide into single Escherichia coli cells and lipid vesicles.

Authors:

Hossain, Farzana and Dohra, Hideo and Yamazaki, Masahito

Journal:

Journal of bacteriology (2021)

Molecular and Spectroscopic Characterization of Green and Red Cyanine Fluorophores from the Alexa Fluor and AF Series*.

Authors:

Gebhardt, Christian and Lehmann, Martin and Reif, Maria M and Zacharias, Martin and Gemmecker, Gerd and Cordes, Thorben

Journal:

Chemphyschem : a European journal of chemical physics and physical chemistry (2021)

Multimodality labeling of NGR-functionalized hyaluronan for tumor targeting and radiotherapy.

Authors:

Li, Xiangyu and Fu, Huaxia and Wang, Jing and Liu, Wei and Deng, Hao and Zhao, Peng and Liao, Wei and Yang, Yuchuan and Wei, Hongyuan and Yang, Xia and Chen, Yue

Journal:

European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences (2021): 105775

Physical properties

Molecular weight	2634.28
Solvent	DMSO

Spectral properties

Absorbance (nm)	654
Correction factor (260 nm)	0.07
Correction factor (280 nm)	0.07
Extinction coefficient (cm ^-1 M ^-1)	250000 ¹
Excitation (nm)	655
Emission (nm)	670
Quantum yield	0.39 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Documents

Protocol
Safety Data Sheet (Catalog 71670)
Safety Data Sheet (Catalog 71671)
Safety Data Sheet (Catalog 71672)
Certificate of Analysis

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Page updated on October 20, 2025