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iFluor® Ultra 647 succinimidyl ester
Fluorescent dye-conjugated antibodies provide a tool for identifying proteins in many applications including fluorescent cell imaging, flow cytometry, western blotting, immunohistochemistry and more. The advantages of using a fluorescently labeled antibody include higher sensitivity, multiplexing capabilities, and ease of use. iFluor® Ultra family is a recent upgrade of our popular iFluor® dyes and optimized for labeling antibodies used for fluorescence imaging and flow cytometry applications. Antibody conjugates prepared with iFluor® Ultra 647 are far superior to the conjugates of other existing similar dyes such as Cy5, Dylight 650 and Alexa Fluor® 647. iFluor® Ultra 647 conjugates are significantly brighter than the conjugates prepared with Cy5, Dylight 650 and Alexa Fluor® 647 under the same conditions. Additionally, the fluorescence of iFluor® Ultra 647 is not affected by pH (4-10). iFluor® Ultra 647 SE dye is reasonably stable and shows good reactivity and selectivity with protein amino groups. iFluor® Ultra 647 has spectral properties and reactivity similar to Cy5, Dylight 650 and Alexa Fluor® 647 (Cy5® and Alexa Fluor® is the trademarks of GE Healthcare and ThermoFisher respectively).
HeLa cells were incubated with mouse anti-tubulin followed by AAT&rsquo;s iFluor<sup>TM</sup>&nbsp;Ultra 647 goat anti-mouse IgG conjugate or Alexa Fluor<sup>&reg;</sup>&nbsp;647 goat anti-mouse IgG.
HeLa cells were incubated with mouse anti-tubulin followed by AAT&rsquo;s iFluor<sup>TM</sup>&nbsp;Ultra 647 goat anti-mouse IgG conjugate or Alexa Fluor<sup>&reg;</sup>&nbsp;647 goat anti-mouse IgG.
CatalogSize
Price
Quantity
716701 mg
Price
71671100 ug
Price
716725 mg
Price
 
Physical properties

Molecular weight2634.28
SolventDMSO
Spectral properties

Absorbance (nm)654
Correction factor (260 nm)0.07
Correction factor (280 nm)0.07
Extinction coefficient (cm -1 M -1)
250000
1
Excitation (nm)655
Emission (nm)670
Quantum yield
0.39
1
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Page updated on October 20, 2025