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iFluor® 790 acid

In vivo fluorescence imaging uses a sensitive camera to detect the fluorescence emission from fluorophores in whole-body living small animals. To overcome the photon attenuation in living tissue, fluorophores with long emission at the near-infrared (NIR) region are generally preferred, including widely used small indocarbocyanine dyes. Recent advances in imaging strategies and reporter techniques for in vivo fluorescence imaging include novel approaches to improve the specificity and affinity of the probes and to modulate and amplify the signal at target sites for enhanced sensitivity. Further emerging developments aim to achieve high-resolution, multimodality, and lifetime-based in vivo fluorescence imaging. Our iFluor® 790 is designed to label proteins and other biomolecules with near-infrared fluorescence. Conjugates prepared with iFluor® 790 have excitation and emission spectra similar to that of indocyanine green (ICG) and the IRDye® 800 dye, with 783/814 nm excitation/emission maxima. iFluor® 790 dye emission is well separated from commonly used far-red fluorophores such as Cy5, Cy7, or allophycocyanin (APC), facilitating multicolor analysis. This fluorophore is also useful for small animal in-vivo imaging applications or other imaging applications requiring NIR detections, such as the two-color western applications with the LI-COR® Odyssey® infrared imaging system.

Example protocol

AT A GLANCE

Important notes
It is important to store at <-15 °C and should be stored in cool, dark place.

It can be used within 24 months from the date of receipt.

PREPARATION OF WORKING SOLUTION

iFluor™ 790 acid working solution:
Add DMF, DMSO or water to make iFluor™ 790 acid working solution of desired concentration.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 790 acid to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM78.23 µL391.151 µL782.301 µL3.912 mL7.823 mL
5 mM15.646 µL78.23 µL156.46 µL782.301 µL1.565 mL
10 mM7.823 µL39.115 µL78.23 µL391.151 µL782.301 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
iFluor® 800 acid80182025000010.1110.030.08
iFluor® 810 acid81182225000010.0510.090.15
iFluor® 820 acid82285025000010.110.16
iFluor® 860 acid85387825000010.10.14
iFluor® 790 Styramide *Superior Replacement for Alexa Fluor 790 tyramide*78781225000010.1310.10.09
iFluor® 840 acid8368792000001-0.20.09
iFluor® 830 acid830867----
iFluor® 670 acid67168220000010.5510.030.033
iFluor® 350 acid3454502000010.9510.830.23
iFluor® 405 acid4034273700010.9110.480.77
iFluor® 430 acid4334984000010.7810.680.3
iFluor® 450 acid4515024000010.8210.450.27
iFluor® 647 acid65667025000010.2510.030.03
Show More (4)

Citations

View all 5 citations: Citation Explorer
Lipid nanoparticulate drug delivery system for the treatment of hepatic fibrosis
Authors: Mukherjee, Swarupananda and Dutta, Ayon and Ash, Dipanjana
Journal: Archives of Hepatitis Research (2021): 001--003
Lipid-based nanoparticle technologies for liver targeting
Authors: B{\"o}ttger, Roland and Pauli, Griffin and Chao, Po-Han and Fayez, Nojoud AL and Hohenwarter, Lukas and Li, Shyh-Dar
Journal: Advanced drug delivery reviews (2020): 79--101
Self-assembly and directed assembly of lipid nanocarriers for prevention of liver fibrosis in obese rats: a comparison with the therapy of bariatric surgery
Authors: Chen, Chun-Han and Chen, Chih-Jung and Elzoghby, Ahmed O and Yeh, Ta-Sen and Fang, Jia-You
Journal: Nanomedicine (2018)
Nanovesicle delivery to the liver via retinol binding protein and platelet-derived growth factor receptors: how targeting ligands affect biodistribution
Authors: Hsu, Ching-Yun and Chen, Chun-Han and Aljuffali, Ibrahim A and Dai, You-Shan and Fang, Jia-You
Journal: Nanomedicine (2017)

References

View all 18 references: Citation Explorer
In Vivo Fluorescence Microscopic Imaging for Dynamic Quantitative Assessment of Intestinal Mucosa Permeability in Mice
Authors: Szabo A, Vollmar B, Boros M, Menger MD.
Journal: J Surg Res. (2007)
A target cell-specific activatable fluorescence probe for in vivo molecular imaging of cancer based on a self-quenched avidin-rhodamine conjugate
Authors: Hama Y, Urano Y, Koyama Y, Kamiya M, Bernardo M, Paik RS, Shin IS, Paik CH, Choyke PL, Kobayashi H.
Journal: Cancer Res (2007): 2791
Fluorescence imaging in vivo: recent advances
Authors: Rao J, Dragulescu-Andrasi A, Yao H.
Journal: Curr Opin Biotechnol (2007): 17
Ex vivo fluorescence imaging of normal and malignant urothelial cells to enhance early diagnosis
Authors: Steenkeste K, Lecart S, Deniset A, Pernot P, Eschwege P, Ferlicot S, Leveque-Fort S, Bri and et R, Fontaine-Aupart MP.
Journal: Photochem Photobiol (2007): 1157
In vivo monitoring the fate of Cy5.5-Tat labeled T lymphocytes by quantitative near-infrared fluorescence imaging during acute brain inflammation in a rat model of experimental autoimmune encephalomyelitis
Authors: Berger C, Gremlich HU, Schmidt P, Cannet C, Kneuer R, Hiest and P, Rausch M, Rudin M.
Journal: J Immunol Methods (2007): 65
Page updated on October 12, 2024

Ordering information

Price
Unit size
Catalog Number1360
Quantity
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Additional ordering information

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Physical properties

Molecular weight

1278.28

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.1

Correction Factor (280 nm)

0.09

Extinction coefficient (cm -1 M -1)

2500001

Excitation (nm)

787

Emission (nm)

812

Quantum yield

0.131

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501

Platform

Fluorescence microplate reader

Excitation782 nm
Emission811 nm
Cutoff790 nm
Recommended plateSolid black
With EDAC or other equivalent activating coupling agents, fluorescent dyes can react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.
With EDAC or other equivalent activating coupling agents, fluorescent dyes can react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.
With EDAC or other equivalent activating coupling agents, fluorescent dyes can react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.